Dimethyl sulphoxide (DMSO), percoll, Nonidet P-40 (NP40), fetal bovine serum (FBS), thimerosal, gambogic acid, trichostatin A (TSA), bovine serum albumin (BSA), carmine-red and Canada balsam were purchased from Sigma-Aldrich (Saint Lous, USA); CellTiter-Glo (CTG) reagent from Promega (Madison, USA); Dulbecco-Modified Eagle’s Medium (DMEM) with or without phenol red, HEPES, L-glutamine from Lonza (Basel, Switzerland); antibiotic-antimycotic reagent (100×) from Thermo Fisher Scientific (Waltham, USA); HDAC1 enzyme (BMLSE456), substrate (BML-KI104) and developer solution BML-KI105) from Enzo Life Sciences, Inc (Farmingdale, USA); Dacinostat (S1095) from Selleckchem (Munich, Germany); the primary monoclonal anti-α-tubulin antibody (DM1A) from Sigma-Aldrich; the anti-acetylated-lysine (Ac-K2-100) from Cell Signaling Technology (Danvers, USA); goat anti-mouse and anti-rabbit IgG (H+L)-horseradish peroxidase secondary antibodies from Bio-Rad Laboratories (Hercules, USA).
Dulbecco s modified eagle medium dmem
Manufactured by Lonza
Sourced in United States, Switzerland, Belgium, United Kingdom, Spain, Germany
Dulbecco's Modified Eagle Medium (DMEM) is a cell culture medium formulated to support the growth and maintenance of various cell types. It provides a balanced salt solution, essential nutrients, and other components required for cell proliferation and survival. DMEM is a widely used base medium in cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Lonza (13 mentions)
Fetal bovine serum (fbs), by Lonza (12 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (12 mentions)
Penicillin streptomycin, by Lonza (9 mentions)
Fetal bovine serum (fbs), by Merck Group (8 mentions)
L glutamine, by Thermo Fisher Scientific (7 mentions)
Penicillin, by Lonza (5 mentions)
Rpmi 1640 medium, by Lonza (5 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (5 mentions)
Streptomycin, by Lonza (4 mentions)
Rpmi 1640, by Lonza (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Trypsin edta, by Lonza (3 mentions)
Lncap, by American Type Culture Collection (3 mentions)
Foetal bovine serum (fbs), by Lonza (3 mentions)
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Fetal calf serum (fcs), by Lonza (3 mentions)
Glutamine, by Thermo Fisher Scientific (3 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (3 mentions)
78 protocols using dulbecco s modified eagle medium dmem
1
Histone Deacetylase Enzyme Assay
2
Cultivation of Human Neuroblastoma and Fibroblast Cell Lines
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The human malignant neuroblastoma cell lines SK-N-BE(2) and SH-SY5Y were a donation by Dr Antonio Serafin, Division of Radiology, Faculty of Medicine and Health Sciences, Stellenbosch University, while, the non-cancerous KMST6 fibroblast was a donation by Prof Mervin Meyer, Department of Biotechnology, University of the Western Cape, South Africa. Cells were cultured in monolayer using Dulbecco Modified Eagles Medium (DMEM, Lonza Group Ltd., Verviers, Belgium), supplemented with 10% foetal bovine serum (FBS, Gibco, Life Technologies Corporation, Paisley, UK) and 1% 100 U/ml penicillin and 100 μg/ml streptomycin (Lonza Group Ltd. Verviers, Belgium). Cells were grown at 37 • C, in a humidified atmosphere of 5% CO2 and 95% air with routine media change. Cells were subcultured at 80% confluency using a solution of 0.25% trypsin EDTA (Lonza Group Ltd., Verviers, Belgium).
3
Quantum Dot-Based Cell Viability Assay
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McCoy’s 5A (modified) Medium was obtained from the American Type Culture Collection (Manassas, VA, USA); a penicillin-streptomycin mixture, trypsin/ethylenediamine tetraacetic acid, phosphate-buffered saline (without Ca2+, Mg2+, or phenol red), and Dulbecco’s Modified Eagle Medium (DMEM) and F12 in a 1:1 mixture with HEPES and L-glutamate was sourced from Lonza (Walkersville, MD, USA); DMEM and mouse antihuman unconjugated monoclonal antibody erbB-2 (HER2) was sourced from Invitrogen (Carlsbad, CA, USA); fetal bovine serum, Tween 20, N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide (EDC) ≥98.0% was purchased from Fluka (St Louis, MO, USA); and N-hydroxysuccinimide (NHS) 98% was obtained from Sigma-Aldrich (St Louis, MO, USA). A Cell Titer-Blue® cell viability assay was obtained from Promega (Madison, WI, USA). CdTe/CdSe/ZnSe quantum dots coated with MUA were synthesized in the laboratory according to our published protocols.53
4
Dendritic Cell Differentiation Protocol
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Dulbecco’s modified Eagle medium (DMEM) and phosphate buffered saline-Ca2+ Mg2+ free (PBS) were obtained from Biowhittaker®, Belgium. Granulocyte macrophage-colony stimulating factor (GM-CSF), phycoerythrin (PE) anti-human CD83 monoclonal antibody (cat# 12-0839, clone: HB15e), fluorescence-activated cell sorting (FACS) buffer and FACS lyse solution were obtained from eBioscience®, Austria. Phycoerythrin hamster anti-mouse CD11c monoclonal antibody (cat# MCA1369T, clone: N418) and fluorescein isothiocyanate (FITC) rat anti-mouse CD83 monoclonal antibody (cat# MCA2747F, clone: 3D11) were obtained from AbD Serotec®, USA. IL-4 was obtained from Sigma Aldrich®, USA. TNF-α was obtained from R&D Systems®, USA. Penicillin–streptomycin solution (Pen/Strep) was obtained from Euro-lone®, Europe. Amphotericin B (Fungizone®) was obtained from Gibco®, USA. Biocoll separating solution, density 1.077 g/mL, was obtained from Biochrom®, Germany. E. purpurea dried whole plant was obtained from Haraz Co., Egypt. Heparin calcium (Cal-heparin®) 5000 U was obtained from Amoun Pharmaceutical Co., Egypt. Human albumin (Zenalb®) 20% was obtained from Bio Products Laboratory Limited, United Kingdom. All other chemicals and reagents were of high analytical grade.
5
Cell Culture of HEK293 and HepG2 Cells
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HEK293 and HEK293/Pank1β cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) (Lonza) supplemented with 10% foetal bovine serum (FBS) (Hyclone), 50 U/ml penicillin and 0.25 μg/ml streptomycin (Lonza). HepG2 cells were cultured in Williams E media (Lonza) supplemented with 10 % FBS, 50 U/ml penicillin and 0.25 μg/ml streptomycin. All cell lines were tested and shown to be free of mycoplasma infection. Generation of HEK293/Pank1β cell line with stable overexpression of Pantothenate kinase 1β was previously reported [50 (link)].
6
Prostate Cancer Cell Lines Cultivation
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The human PCa cell lines LNCaP, VCaP, 22Rv1, C4-2B, PC3, and DU145 were purchased from the American Type Culture Collection (Rockville, MD, USA). Roswell Park Memorial Institute (RPMI) 1640 medium and Dulbecco's Modified Eagle Medium (DMEM) were purchased from Lonza (Basel, Switzerland). Cells were routinely maintained in a humidified incubator with 5% (v/v) CO2 and 95% (v/v) air at 37°C in RPMI 1640 medium (LNCaP, C4-2B, 22Rv1, and PC3 cells) or DMEM (VCaP and DU145 cells) containing 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA), 5 mg ml−1 penicillin/streptomycin, and 2 mmol l−1 L-glutamine (Lonza). For hormone responsiveness experiments, cells were plated in complete medium containing 10% (v/v) FBS and were then preincubated in medium containing 5% (v/v) charcoal-treated (CT)-FBS for 2 days before induction with the synthetic androgen R1881 (1 nmol l−1; Merck, Kenilworth, NJ, USA) for the indicated time periods. All cell lines were routinely tested and found to be negative for mycoplasma contamination.
7
Investigating Cellular Responses Comprehensively
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Dulbecco's Modified Eagle Medium (DMEM), penicillin/streptomycin and fetal bovine serum (FBS) were purchased from Lonza (Verviers, Belgium). FITC-dextran (42 kDa), Hoechst 33342, LPS and collagen were from Sigma-Aldrich (St. Louis, MO, USA). OneComp beads were from eBioscience. BD OptEIA mouse TNF ELISA kit, BD OptEIA mouse IL-6 ELISA kit and Human inflammatory bead assay CBA was from BD Biosciences (San Diego, CA, USA). Interleukin 4 (IL-4), Interleukin 10 (IL-10), interferon-γ (INFγ) and granulocyte macrophage colonystimulating factor (GM-CSF) were from ImmunoTools (Germany). Millecell EZ slides and Mowiol were from Millipore (Hayward, CA, USA) and Upcell plates were purchased from Nunc (Rochester, NY, USA). H 2 DCFDA-CM, DHE, N-acetyl-L-cysteine (NAC), propidium iodide, Hoechst and RNase were from, Life technologies (Grand Island, NY, USA). and Rat IgG2aκ Iso Control APC were from eBioscience (San Diego, CA, USA). γH2AX and LC3B were from cell signaling (Beverly, MA, USA) and anti-rabbit Alexa Fluor 647 were from molecular probes (Life Technology, Grand Island, NY, USA).
8
Isolation and Culture of Decidual Mesenchymal Stem Cells
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Human placentas from healthy mothers were supplied by the Department of Obstetrics and Gynecology under written consent previously approved by the Ethics Committee at the Hospital Universitario 12 de Octubre. DMSC isolation and culture was performed as previously described [42 (link)]. Briefly, placental membranes were digested with trypsin-versene (Lonza, Spain), and the cells were seeded at 1.2 × 105 cells/cm2 and cultured at 37 °C, 5 % CO2 and 95 % humidity in Dulbecco’s modified Eagle medium (DMEM; Lonza) supplemented with 2 mM L-glutamine, 0.1 mM sodium pyruvate, 55 μM B-mercaptoethanol, 1 % nonessential amino acids, 1 % penicillin/streptomycin, 10 % fetal bovine serum and 10 ng/ml epidermal growth factor 1 (EGF-1; Sigma-Aldrich Química, Spain). The morphology, phenotype and MSC characteristics of DMSCs have been previously reported [42 (link)]. Cells were cryopreserved and, before use, were thawed and passaged at a density of around 5 × 104 cells/cm2 until passage 6–8.
9
Isolation and Characterization of SARS-CoV-2 Variants
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B.6 lineage isolation of SARS-CoV-2 has been described earlier [28 (link)]. In this study, 5 variants of SARS-CoV-2 were used. The original Wt-SARS-CoV-2 isolate was designated as USA-WA1/2020, NR-52281, and its accession no is MN985325, GISAID: EPI_ISL_404895, GenBank: MT020880. The B.1.1.7 (α-variant) from the United Kingdom denoted as USA/CA_CDC_5574/2020, NR-54011, and genebank: GISAID is EPI_ISL_751801. South African origin B.1.351 (β-variant) isolate comprises of hCoV-19/South Africa/KRISPK005325/2020, NR-54009, and its gene accession no is GISAID: EPI_ISL_678615. The Indian origin B.1.617.2 (δ-variant) isolate is known as THSTI_287, and its gene accession no is GenBank: MZ356566.1 [29 (link)]. The USA origin B.1.617 (κ-variant) is named as USA/CA-Stanford-15_S02/2021, and its gene accession no is GenBank: GISAID: EPI_ISL_1675223. Vero E6 (CRL1586) and Calu-3 (HTB55) cell lines were cultured in Dulbecco's modified Eagle medium (DMEM; Lonza) supplemented with 10 % fetal bovine serum (FBS; Himedia), 100 U/ml of penicillin, 100 μg/ml of streptomycin. All SARS-CoV-2 variants were propagated in Vero E6 cells or Calu-3 cells and virus passaging was restricted to 4–8 passages only. All the virus stocks were authenticated by whole genome sequencing as described in earlier information [27 (link),30 (link)].
10
Acetylcholinesterase Activity Assay
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Dulbecco’s modified Eagle medium (DMEM), fetal bovine serum (FBS), trypsin-EDTA, and phosphate-buffered saline (PBS) pH 7.4 were obtained from Lonza (Basel, Switzerland); rIL-6 was produced inhouse as reported [19 (link),20 (link)]. Chemiluminescent HRP substrate kit and centrifugal filter units (Amicon Ultra 10K) were obtained from Millipore (Burlington, MA). Acetylcholinesterase from Electrophorus electricus (AChE) and acetylthiocholine, 5’,5’-dithiobis-2-nitrobenzoic acid (DTNB) were purchased from Sigma-Aldrich.
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