The radiolabeled products were analyzed by TLC using a G60 silica gel plate (Merck, Germany). The plate was developed by a 2:1 solvent mixture of acetonitrile (ACN) and water. The TLC plate was exposed to an imaging plate (GE Healthcare, Chicago), and the imaging plate was scanned by Typhoon FLA 7000 (GE Healthcare).
Imaging plate
Manufactured by GE Healthcare
Sourced in United States, United Kingdom
The Imaging Plate is a digital X-ray detector used in medical imaging. It converts X-ray energy into a latent image that can be digitally processed and displayed on a computer screen. The Imaging Plate serves as a core component in various radiographic imaging modalities.
Automatically generated - may contain errors
Lab products found in correlation
Typhoon fla 7000, by GE Healthcare (2 mentions)
Storm 860, by GE Healthcare (1 mentions)
Amersham typhoon 5 biomolecular imager, by GE Healthcare (1 mentions)
Typhoon 9200, by GE Healthcare (1 mentions)
Typhoon fla 9500, by GE Healthcare (1 mentions)
Imagequant tl, by GE Healthcare (1 mentions)
Multi gauge, by Fujifilm (1 mentions)
Tissue tek optimal cutting temperature compound, by Sakura Finetek (1 mentions)
Image gauge version 4, by Fujifilm (1 mentions)
Fla 5000 image reader, by Fujifilm (1 mentions)
4 protocols using imaging plate
1
Radiolabeled Products Analysis by TLC
2
Protein Analysis by SDS-PAGE and Imaging
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Radiolabeled proteins were analyzed by SDS-PAGE followed by radioimaging with Imaging Plate and image analyzers, Typhoon FLA-7000 (GE Healthcare), Typhoon FLA-9500 (GE Healthcare), and Typhoon 9200 (GE Healthcare). Signals of immunoblotting were detected with image analyzers, Typhoon FLA-7000 (GE Healthcare), Typhoon 9200 (GE Healthcare), Storm 860 (Molecular Dynamics), and Amersham Typhoon 5 Biomolecular imager (GE Healthcare). Blots/gel images were analyzed by using software, ImageQuant TL (GE Healthcare), Adobe Photoshop, Adobe Illustrator, multi gauge (Fuji Film), and Image J (Wayne Rasband).
3
Quantifying Arterial Radiographic Intensity
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After the biodistribution study, the excised myocardium and the carotid arteries (right and left) were embedded in Tissue-Tek optimal cutting temperature compound (Sakura Finetek Japan, Tokyo, Japan).
Frozen microsections (20 μm thick) were placed on glass slides, dried, and exposed to an imaging plate (GE Healthcare, Chicago, IL, USA). The imaging plate was read using an FLA-7000 imaging plate reader (GE Healthcare). The radiographic intensities of the myocardium and arteries were measured using ImageJ software (National Institutes of Health, Bethesda, MD, USA). 28 sections of the uninjured artery and 32 sections of the injured artery were processed, and the ratio of arterial intensity to myocardial intensity was recorded for both uninjured and injured arteries.
Frozen microsections (20 μm thick) were placed on glass slides, dried, and exposed to an imaging plate (GE Healthcare, Chicago, IL, USA). The imaging plate was read using an FLA-7000 imaging plate reader (GE Healthcare). The radiographic intensities of the myocardium and arteries were measured using ImageJ software (National Institutes of Health, Bethesda, MD, USA). 28 sections of the uninjured artery and 32 sections of the injured artery were processed, and the ratio of arterial intensity to myocardial intensity was recorded for both uninjured and injured arteries.
4
Radiolabeling and Imaging of Grafted Plants
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Intact Nb plants, Nb homografts and Nb/At heterografts were conducted to the radio isotope experiments. All leaves except for three to four pieces of leaves in intact or scion plants were removed, and then the stem was cut at its base and placed in a 5 ml tube that contained 2 ml of distilled water, Pi (0.1 µM), and 32 P-phosphate (10 kBq). After 6 h incubation at 27℃, the distribution of 32 P in the plant was visualized by radioluminography using an imaging plate (GE Healthcare UK, Buckinghamshire, UK) and a FLA-5000 image reader (Fujifilm, Tokyo, Japan). The amount of 32 P was calculated with the image analysis software (Image Gauge version 4.0, Fujifilm). For the timecourse analysis, we employed the RRIS (Sugita et al., 2016) , which enable us to observe 32 P distribution in the plant sequentially. The activity of 32 P-phosphate in the incubation solutions was 30 kBq for Nb and Nb/Nb and 60 kBq for Nb/At. The radioactivity image was captured during the dark period of a 15 min light/dark cycle. The accumulation of 32 P in the scion and stock was examined in two sections (10x10 cm in size) collected above and below the graft union, respectively.
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