To check ability of HABN to target the inflamed site in the colon, a 1 day after preparing colitis mice or heathy mice by giving 3% DSS water or normal water for five days, 7.5 mg/kg (0.3% 1.8 μg Cy 5.5;) of HABN-Cy5.5, HACN-Cy5.5, or HA-Cy5.5 was orally administered into the mice. After 6 h, mice were euthanized, and organs including heart, kidney, lung, spleen, liver, stomach, small intestine and colon were excised. Fluorescence intensities in organs from each group were analyzed using a Xenogen IVIS Lumina in vivo imaging system (PerkinElmer) with a Cy 5.5 filter channel and an exposure time of 5 s.
Xenogen ivis lumina
Manufactured by PerkinElmer
Sourced in United States, United Kingdom, Azerbaijan
The Xenogen IVIS Lumina is a non-invasive, in vivo imaging system designed for preclinical research applications. It enables the visualization and quantification of bioluminescent and fluorescent reporters within small animal models.
Automatically generated - may contain errors
Lab products found in correlation
D luciferin, by PerkinElmer (6 mentions)
Living image, by PerkinElmer (6 mentions)
D luciferin potassium salt, by GoldBio (2 mentions)
Viscotears gel, by Novartis (2 mentions)
Isoflurane, by Abbott (2 mentions)
Living image software, by PerkinElmer (2 mentions)
Female c57bl 6 mice, by Jackson ImmunoResearch (2 mentions)
Phamfloxed, by Jackson ImmunoResearch (1 mentions)
Celltiter glo, by Promega (1 mentions)
Tumor dissociation kit, by Miltenyi Biotec (1 mentions)
D luciferin substrate, by PerkinElmer (1 mentions)
Matrigel, by Corning (1 mentions)
D luciferin, by Promega (1 mentions)
L 012, by Fujifilm (1 mentions)
Living image 3, by PerkinElmer (1 mentions)
Living image version 4, by PerkinElmer (1 mentions)
Nod cg prkdcscid il2rgtm1wjl szj, by Jackson ImmunoResearch (1 mentions)
C57bl 6j female mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
32 protocols using xenogen ivis lumina
1
Targeted Delivery of HABN in Colitis
2
Tracking CDCs in Ischemia-Reperfusion
3
Luminescent Monitoring of Breast Cancer Recurrence
4
Intratumoral Delivery of NCTD Gel
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After establishing the Hepa 1-6 tumor-bearing mice model according to the above method, NCTD Gel loaded with Cy7 and free Cy7 were injected through intratumorally, respectively. At different time points after injection, Xenogen IVIS Lumina imaging system (Perkin Elmer Inc.) was used to compare the retention effect of NCTD Gel and Cy7 in the tumor.
5
Evaluating CAR T Cell Therapy for NALM6 Leukemia
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Luciferase-expressing NALM6-GPC2 (0.5 × 106) and NALM6-CD276 (0.5 × 106) cells were mixed and i.v. injected into NSG mice. Three days later, mock T cells (2.5 × 106 or 5 × 106) of each type of CAR T cell were injected to treat the mice. NALM6 leukemia was detected using the Xenogen IVIS Lumina (PerkinElmer) every 3 or 4 days. Twenty-one days after CAR T cell infusion, mice treated with 5 × 106 CAR T cells were euthanized for spleen collection, and splenic cells were used for flow cytometric analysis of NALM6 cells remaining in the spleen and of T cells phenotype.
6
In Vivo Bioluminescence Imaging of Murine Eyes
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All mice used for live imaging were aged between 12 and 25 weeks. For imaging, mice were anesthetized using 1.5 to 2% isoflurane (Abbott Laboratories Ltd., Berkshire, UK) in approximately 1.5 L/min flow of oxygen. A mix of luciferin substrate (30 mg/mL D-luciferin potassium salt; Gold Biotechnology, St. Louis, MO, USA) mixed 1:1 w/v with Viscotears gel (Novartis, Camberley, UK) was dropped onto the eye of heterozygous Krt12+/luc2 transgenic mice immediately prior to imaging. A Xenogen IVIS Lumina (Perkin Elmer, Cambridge, UK) was used to quantify luminescence. A region of interest encircling the mouse eye was selected for quantification whose size and shape was kept constant throughout, using protocols as previously described.9 (link)
7
Targeted Delivery of HABN in Colitis
8
Mitochondrial Isolation and Biodistribution
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For in vitro experiments, mitochondria were isolated from HEK293 cells expressing a GFP targeted to mitochondria tag (addgene, plasmid #50057, Watertown, MA), whereas for in vivo experiments mitochondria were isolated from liver of PhAMexcised (Jackson Laboratory Stock No: 018397) or PhAMfloxed (Jackson Laboratory Stock No: 018385) crossed with Alb1-cre (Jackson Laboratory Stock No: 016832) mice using a previously described protocol13 . Cells (or liver) were digested in ice-cold isolation buffer containing 300 nM sucrose, 10 mM HEPES, 1 mM EGTA and 2 mg/mL subtilisin A protease (Sigma-Aldrich, St. Louis, MO) and the homogenate was centrifuged at 500 × g for 5 min at 4°C. To separate mitochondria from other cellular debris, the supernatant was centrifuged at 800 × g for 10 min and again at 3000 × g for 10 min at 4°C. The mitochondrial pellet was reconstituted in cold sterile 1X PBS, and the protein concentration equivalent was quantified by Bradford assay. Functional viability of isolated mitochondria was assessed using CellTiter-Glo (Promega) to measure ATP concentrations. Mitochondrial uptake into organs was determined ex vivo using epifluorescent bioimaging (Xenogen IVIS Lumina, Perkin Elmer Akron, OH, USA) at 30 min, 2 h, 24 h, 48 h, or 72 h following a single 20 μg/g mitochondrial delivery.
9
In Vivo Corneal Luminescence Imaging
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All mice used for live imaging were aged between 12 and 25 weeks old. For imaging, mice were anaesthetised using 1.5–2% isoflurane (Abbott Laboratories Ltd., Berkshire, UK) in ~1.5 l/min flow of oxygen. A mix of luciferin substrate (30 mg/ml D-luciferin potassium salt; Gold Biotechnology, St. Louis, USA) mixed 1:1 w/v with Viscotears gel (Novartis, Camberley, UK) was dropped onto the eye of heterozygous Krt12 +/luc2 transgenic mice immediately prior to imaging. A Xenogen IVIS Lumina (Perkin Elmer, Cambridge, UK) was used to quantify luminescence. Live images of mice (n = 4) were taken every 24 hours for 7 days, then once every week thereafter for six weeks (42 days) in total. Quantification of luciferase inhibition was determined by calculating the right/left ratio, with values normalised to those at day 0 (as 100%).
10
Biodistribution of Cypate-Loaded Nanoparticles
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Depilated C57BL/6 mice were subcutaneously inoculated in the dorsal right side with 1 × 106 B16F10 cells, and tumors were allowed to become established over time. When the tumor volume reached approximately 100 mm3, free cypate or SP3NPs at an equivalent cypate dose of 1 mg kg-1 was injected through the tail vein. At predetermined time points post-administration, the in vivo distribution of fluorescent free cypate and SP3NPs within the mice was assessed using a Xenogen IVIS Lumina imaging system (Perkin Elmer Inc.) with a built-in ICG filter set and an exposure time of 5 s. The ex vivo biodistribution patterns of cypate and SP3NPs were evaluated by a fluorescence imaging system as well. In some experiments, tumors were excised and cryosectioned, and the intratumoral distribution of free cypate or SP3NPs was assessed using confocal microscopy, as described above.
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