The powder of the leaves, 50.0 mg, was precisely weighed into a 15 mL capped centrifuge tube; 10 mL of 20% MeOH was then transferred to the tube. The suspension was vortexed shortly prior to an ultrasonic extraction for a continuous 10 min. And, prior to injection, all the solutions were subjected to membrane filtration by 0.22 µm Minisart SRP (polytetrafluoroethylene) syringe filters (Sartorius Stedim Biotech GmbH, Goettingen, Germany).
Minisart srp
Manufactured by Sartorius
Sourced in Germany
The Minisart SRP is a syringe filter designed for sample preparation. It is a single-use filter that is used to remove particles and microorganisms from liquids prior to analysis or further processing.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Diethyl ether, by Thermo Fisher Scientific (1 mentions)
Mem neaa 100, by Thermo Fisher Scientific (1 mentions)
Glass coverslip, by Thermo Fisher Scientific (1 mentions)
α cyano 4 hydroxycinnamic acid hcca, by Merck Group (1 mentions)
Trifluoroacetic acid tfa, by Carl Roth (1 mentions)
Proteostat amyloid plaque detection kit, by Enzo Life Sciences (1 mentions)
A549 cells, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
4 protocols using minisart srp
1
Leaf Powder Extraction and Filtration
2
Peptide Synthesis and Characterization
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OymaPure,
Arg(Pbf)-OH, Fmoc-Gly-OH, Fmoc-Asp(OtBu)-OH,
Fmoc-Cys(Trt)-OH Fmoc-Lys(Boc)-OH, Fmoc-Phe-OH, Fmoc-Gln(Trt)-OH,
and Fmoc-Phe-Wang resin were purchased from Novabiochem. N-Ethyldiisopropylamine (DIPEA), piperidine (≥99.5% for peptide
synthesis), and trifluoroacetic acid (TFA, ≥99.9%) were obtained
from Carl Roth. Dimethylformamide (DMF for peptide synthesis), diethyl
ether, and dimethyl sulfoxide (DMSO, ≥99.97+%) were purchased
from Acros Organics. Acetonitrile (HPLC grade) was purchased from
Fisher Scientific. Syringe filters Minisart SRP (0.20 μm) were
obtained from Sartorius. Glass coverslips (24 × 50 mm) were obtained
from Hirschmann and Glass coverslips (Ø = 13 mm) were purchased
from Fisher Scientific. ITO-coated glass slides for scanning electron
microscope (SEM) (15 × 20 mm) were obtained from Ossila and for
MALDI-MSI (25 × 75 mm) were purchased from Bruker Daltonics.
LE Agarose was obtained from Biozym Scientific. A549 cells, Dulbecco’s
Modified Eagle’s Medium (DMEM, 4.5 g/L glucose/glutamine),
penicillin/streptavidin, fetal bovine serum (FBS), and minimum essential
medium non-essential amino acids (MEM NEAA, 100×) were purchased
from Thermo Fisher Scientific. The ProteoStat Amyloid Plaque detection
Kit was purchased from Enzo Life Sciences, Inc. α-Cyano-4-hydroxycinnamic
acid (HCCA) was purchased from Sigma-Aldrich.
Arg(Pbf)-OH, Fmoc-Gly-OH, Fmoc-Asp(OtBu)-OH,
Fmoc-Cys(Trt)-OH Fmoc-Lys(Boc)-OH, Fmoc-Phe-OH, Fmoc-Gln(Trt)-OH,
and Fmoc-Phe-Wang resin were purchased from Novabiochem. N-Ethyldiisopropylamine (DIPEA), piperidine (≥99.5% for peptide
synthesis), and trifluoroacetic acid (TFA, ≥99.9%) were obtained
from Carl Roth. Dimethylformamide (DMF for peptide synthesis), diethyl
ether, and dimethyl sulfoxide (DMSO, ≥99.97+%) were purchased
from Acros Organics. Acetonitrile (HPLC grade) was purchased from
Fisher Scientific. Syringe filters Minisart SRP (0.20 μm) were
obtained from Sartorius. Glass coverslips (24 × 50 mm) were obtained
from Hirschmann and Glass coverslips (Ø = 13 mm) were purchased
from Fisher Scientific. ITO-coated glass slides for scanning electron
microscope (SEM) (15 × 20 mm) were obtained from Ossila and for
MALDI-MSI (25 × 75 mm) were purchased from Bruker Daltonics.
LE Agarose was obtained from Biozym Scientific. A549 cells, Dulbecco’s
Modified Eagle’s Medium (DMEM, 4.5 g/L glucose/glutamine),
penicillin/streptavidin, fetal bovine serum (FBS), and minimum essential
medium non-essential amino acids (MEM NEAA, 100×) were purchased
from Thermo Fisher Scientific. The ProteoStat Amyloid Plaque detection
Kit was purchased from Enzo Life Sciences, Inc. α-Cyano-4-hydroxycinnamic
acid (HCCA) was purchased from Sigma-Aldrich.
3
Extraction of Compounds from Horse Hair
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Horse or pony hair (0.3 g) was placed in a 15 mL centrifuge tube, pentane (7 mL) was added. The tube was then stored in a refrigerator for 3 days, after which the tube was shaken (30 min, Excella E2 Platform shaker, New Brunswik Scientific, North Hampton, NH, USA), vortexed (30 sec, Genie 2 Vortex mixer 12-812, Fisher Scientific, Hampton, NH, USA), filtered (Whatman qualitative filter paper, Grade 1, diam. 90 mm, Sigma-Aldrich Corp., St. Louis, MO, USA), and dried (Na 2 SO 4 ). An aliquot of 2 mL was filtered using a syringe filter (0.45 µm, Minisart SRP, Sartorius Stedim Biotech GmbH, Göttingen, Germany). This procedure was repeated using solvents: n-hexane, CH 2 Cl 2 , acetone, EtOAc, and ethanol.
4
Aqueous Extraction of Biomass Compounds
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An amount of BC was weighed (1 g ± 0.01 mg) into 20 ml vials and 10 ml of deionized water (DW, HPLC grade) was added. The vials were sealed with aluminum crimp seals with PTFE/rubber septa. The sealed vials were then placed on a mechanical shaker (IKA KS 260) covered with an aluminum foil, and left shaking at 150 rpm for 72 hours at ambient temperature.
The resulting solutions were centrifuged at 3800 rpm for 10 min (ALC4232 centrifuge) to separate the solid material and filtered with PTFE syringe filters 0.45µm (Sartorius Minisart SRP) thereafter.
The resulting solutions were centrifuged at 3800 rpm for 10 min (ALC4232 centrifuge) to separate the solid material and filtered with PTFE syringe filters 0.45µm (Sartorius Minisart SRP) thereafter.
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