Pravastatin sodium salt

Atorvastatin calcium salt, rosuvastatin calcium salt, simvastatin sodium salt and pravastatin sodium salt (Cayman Chemical, Ann Arbor, MI, USA), curcumin, and RG108 (Abcam, Cambridge, UK) were dissolved in dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St. Louis, MO, USA) to a concentration of 10 mM and stored at −20 °C for short-term use or at −80 °C for long-term use. Sodium butyrate (Selleck Chemicals, Houston, TX, USA) was dissolved in ultrapure distilled water to a concentration of 50 mM and stored at −20 °C.

Atorvastatin sodium salt (>98%), pravastatin sodium salt (≥98%), fluvastatin sodium salt hydrate (≥98%) and simvastatin (≥98%) were purchased from Cayman Chemical (Ann Arbor, MI, USA). Rosuvastatin sodium salt (98%) was synthesized by Synthex Technologies Sp. z o. o. according to previously reported procedure [40 (link)]. Potassium phosphate dibasic (K₂HPO₄) (≥98%) and potassium phosphate monobasic (KH₂PO₄) (≥98%) were purchased from J.T. Baker (New Jersey, NY, USA). Disodium edetate dihydrate (EDTA) (>98.5%), 4-(2-Hydroxyethyl)piperazin-1-ylethanesulfonic acid (HEPES) (99%), ethanol (99.8%), perchloric acid (HClO₄) (70%), magnesium chloride hexahydrate (MgCl₂ × 6H₂O) (99%) and potassium hydroxide (KOH) were purchased from Avantor Performance Materials (Gliwice, Poland). Adenosine 5′-monophosphate disodium salt (AMP) (≥99.9%), adenosine 5′-diphosphate sodium salt (ADP) (≥95%), adenosine 5′-triphosphate disodium salt hydrate (ATP) (99%), tetrabutylammonium hydrogensulfate (TBA) (97%), methanol (≥99.9%), isopropyl thiogalactopyranoside (IPTG), bacterial cell culture reagents and supplements for adenylate kinases production, BL21-CodonPlus (DE3)-RIL strain and pET-3a(+) expression vector were purchased from Sigma-Aldrich (Poznań, Poland).