MJ33 (as the lithium salt), 2-thiobarbituric acid (TBA), and butylated hydroxytoluene were purchased from Sigma-Aldrich (St. Louis, MO). 4′,6-Diamidino-2-phenylindole (DAPI) and Dulbecco's phosphate-buffered saline (DPBS) were obtained from Life Technologies (Grand Island, NY). Medical grade O2 was obtained from Air Products (Allentown, PA). Ferrous oxidation-xylenol orange (FOX) assay kit was from Northwest Life Science (Vancouver, WA). Trichloroacetic acid and formalin (10%) were purchased from Fisher Scientific (Fair Lawn, NJ). Coomassie blue protein assay reagent was purchased from Bio-Rad Laboratories (Hercules, CA). Proteinase K was purchased from DAKO (Carpinteria, CA). Terminal transferase dUTP nick-end-labeling (TUNEL) Label and TUNEL Enzyme were purchased from Roche (Indianapolis, IN).
Coomassie blue protein assay reagent
Manufactured by Bio-Rad
Sourced in United States
Coomassie blue protein assay reagent is a colorimetric assay used to quantify the total protein concentration in a sample. It works by binding to proteins and producing a blue color change, which can be measured using a spectrophotometer.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s phosphate buffered saline dpbs, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Formalin, by Thermo Fisher Scientific (1 mentions)
Trichloroacetic acid, by Thermo Fisher Scientific (1 mentions)
Butylated hydroxytoluene, by Merck Group (1 mentions)
Tunel enzyme, by Roche (1 mentions)
Proteinase k, by Agilent Technologies (1 mentions)
2 thiobarbituric acid tba, by Merck Group (1 mentions)
2 protocols using coomassie blue protein assay reagent
1
Oxidative Stress and Cell Apoptosis
2
TER Activity Measurement Protocol
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TER activity was measured as described previously [12 (link)]. The reaction mixture contained 100 mM potassium phosphate buffer, pH 6.2, 0.4 mM NADPH, 0.5 mM crotonyl-CoA, and enzyme. The oxidation of NADPH was followed by a decrease in absorbance at 340 nm (ε = 6.22 mM-1 cm-1). The protein concentration of enzyme solution was measured using the Coomassie Blue protein assay reagent (Bio-Rad Laboratories, Hercules, CA, USA).
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