Human embryonic kidney (HEK) 293 T cells were obtained from ATCC and cultured in IMDM medium (Euroclone) supplemented with 10% heat-inactivated fetal bovine serum (Biowhittaker), 1 mM L-glutamine and penicillin/streptomycin (100 μg/ml) (Euroclone). As described by Pollard et al. [13 (link)], G144 human glioma neural stem (GNS) cell lines were maintained onto laminin (10 mg/ml, Sigma) coated dishes in expansion media (RHA-B media from Stem Cell Technologies) supplemented with 20 ng/mL of EGF (recombinant mouse, PeproTech 315–09 20 ng/ml) and 20 ng/mL bFGF (recombinant human, PeproTech100-18B, 20 ng/ml). Medium was changed every 2 days, and cells were split 1:2 to 1:3 once culture became confluent.
Imdm medium
Manufactured by Euroclone
Sourced in Italy
IMDM medium is a cell culture medium designed for the growth and maintenance of a variety of cell types, including mammalian and insect cells. It is a complex mixture of amino acids, vitamins, inorganic salts, and other components that provide the necessary nutrients for cell proliferation and survival. The core function of IMDM medium is to support the in vitro culture of cells, enabling researchers to study their behavior, metabolism, and other characteristics in a controlled laboratory environment.
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Lab products found in correlation
Penicillin streptomycin, by Euroclone (2 mentions)
L glutamine, by Euroclone (2 mentions)
Laminin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Knockout serum replacement (ksr), by Thermo Fisher Scientific (1 mentions)
Imdm medium, by Thermo Fisher Scientific (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
A2780 cells, by American Type Culture Collection (1 mentions)
Dmem high glucose, by Merck Group (1 mentions)
Rpmi 1640, by Euroclone (1 mentions)
Polybrene, by Merck Group (1 mentions)
Dihydrocortisone, by Merck Group (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Rpmi medium, by Lonza (1 mentions)
4 protocols using imdm medium
1
Culturing HEK293T and Glioma Stem Cells
2
Culturing and Maintaining Cell Lines
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All cells were grown at 37°C in a humidified air atmosphere with 5% CO2 unless specified. mES were grown on gelatine and feeder layers, using DMEM (high glucose) (Invitrogen) supplemented with 15% knockout serum replacement (Invitrogen), LIF (1,000 U/ml), 0.1 mM nonessential amino acids, 1% glutamax, and 55 mM β‐mercaptoethanol. Wild‐type mES cells (R1) were obtained from the American Type Culture Collection (ATCC). ES Cas9 clones and loss‐of‐function libraries were previously generated (Ruiz et al, 2016 (link)). Human cancer cell lines HEK‐293T, DLD‐1, and HeLa (ATCC) were cultured in standard DMEM (high glucose) (Sigma, D5796) supplemented with 10% FBS and 1% penicillin/streptomycin. A2780 cells (ATCC) were maintained in RPMI 1640 and IMDM medium (EuroClone, ECM2001L), 10% FBS, and 1% penicillin/streptomycin, respectively. For KBM7Cas9 cells (kind gift of Cristina Mayor‐Ruiz), IMDM medium (Invitrogen) containing 15% FBS and 1% penicillin/streptomycin was used. For the analysis of CHOP transcription, DLD‐1 cells were infected with pCLX‐CHOP‐dGFP lentiviruses (Addgene, 71299), single‐cell isolated, and selected on the basis of expressing dGFP in response to tunicamycin. All cell lines used in this study were routinely tested for Mycoplasma contamination.
3
Lentiviral Transduction of GL261 Cells
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For the infection, 1 × 105 GL261 cells were seeded in 6-well plate and 2 h before infection growing medium was replaced with IMDM medium (Euroclone, Milan, Italy) supplemented with 10% of FBS HyClone (GE Healthcare, Chicago, IL, USA), 1% penicillin/streptomycin, and 1% l -glutamine (infection medium). After 2 h, cells were infected with the vector (pCCL.PGK.luciferase.WPRE-PLW-vector, kindly provided by Dr. Stefano Rivella, The Children’s Hospital of Philadelphia, PA, USA) with a MOI of 5, in presence of polybrene (8 µg/ml) (Sigma-Aldrich, Saint Louis, MO, USA). Cells were washed 16 h later in order to remove all viral particles, then cells were cultured normally. After having been acquired at CCD camera to evaluate luciferase production, cells were implanted in C57BL/6 mice.
4
Cell Culture Protocols for Hematological Cell Lines
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For detailed description, please refer to https://web.expasy.org/cellosaurus/ . Briefly: K562: erythroleukemia immortalized myelogenous cell line. MEG-01: human megakaryoblastic leukemic cell line. SUP-B15: Human B cell precursor leukemia. HEL: Human Erythroleukemic cell line. SET-2: human essential thrombocythemic. UKE1: human essential thrombocythemic cells. B-ALL BCR-ABL1+ GFP+: cell line established from Cdkn2a−/− murine bone marrow cells infected with a pMIG retrovirus expressing a p190BCR-ABL1-GFP cassette (a gift from Prof. Ghysdael, Paris Diderot University). K562 and HEL cell lines were grown in RPMI medium (Lonza) supplemented with 10% of FBS (Fetal Bovine Serum) (Sigma); MEG-01 and SET-2 were grown in RPMI medium (Lonza) supplemented with 20% of FBS (Fetal Bovine Serum); UKE1 were grown in IMDM medium (EuroClone) supplemented with 10% FBS (Fetal Bovine Serum) (Sigma) and 10% Horse Serum (Invitrogen), 1uM di HydroCortisone (Sigma); SUP-B15 were grown in RPMI medium (Lonza) supplemented with 10% of FBS (Usa Origin Sigma); B-ALL BCR-ABL1+ GFP+ were grown in RPMI (Lonza) with 15% FBS (Sigma) and 7,5% NaHCO3. The medium of all the cell lines was supplemented with 100 μg/ml penicillin/streptomycin (EuroClone), 4mM L-glutamine (EuroClone). Cells were grown at an optimal concentration of 0,5 × 106 cells/mL, in a humidified 5% CO2 atmosphere at 37 °C.
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