Flow cytometry was used to analyze the percentage of T-cell subsets CD4+ and CD8+ in the splenocytes of mice from the four groups (pVAX1-rMYR1, pVAX1, PBS, and healthy control). Splenocyte suspensions (5 × 105 cells/ml) were dually stained with anti-mouse CD3e-FITC + anti-mouse CD8-PE and anti-mouse CD3e-FITC + anti-mouse CD4-PE (eBioscience, United States) for 30 min at 4°C in the dark. Cell population analysis was conducted with the FACScan flow cytometer using the CellQuest software (BD Biosciences, Franklin Lakes, NJ, United States).
Anti mouse cd3e fitc anti mouse cd8 pe
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Anti-mouse CD3e-FITC + anti-mouse CD8-PE is a laboratory reagent used for the identification and characterization of mouse T cells. It contains two monoclonal antibodies, one conjugated with FITC (fluorescein isothiocyanate) that binds to the CD3e antigen, and the other conjugated with PE (phycoerythrin) that binds to the CD8 antigen. This reagent can be used in flow cytometry applications to detect and analyze the presence of CD3e+ and CD8+ T cells in mouse samples.
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Lab products found in correlation
Cellquest software, by BD (5 mentions)
Anti mouse cd3e fitc anti mouse cd4 pe, by Thermo Fisher Scientific (4 mentions)
Facscan flow cytometer, by BD (4 mentions)
Anti mouse cd3e fitc anti mouse mhc 1 pe, by Thermo Fisher Scientific (3 mentions)
Anti mouse cd3e fitc anti mouse mhc 2 pe, by Thermo Fisher Scientific (3 mentions)
Facscan flow cytometry, by BD (1 mentions)
Anti mouse cd4 pe, by Thermo Fisher Scientific (1 mentions)
Anti mouse cd3e fitc, by Thermo Fisher Scientific (1 mentions)
5 protocols using anti mouse cd3e fitc anti mouse cd8 pe
1
Murine T-cell Subset Analysis
2
Splenocyte Immunophenotyping in Mice
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The levels of CD4+ and CD8+ T cell subsets and the levels of MHC-I and MHC-II molecules in the splenocytes of mice from the four test groups, pVAX-EF-1α, pVAXI, PBS, and blank, were determined using flow cytometry as previously described [26 (link)]. Splenocyte suspensions (1 × 106 cells/ml) were dually stained with anti-mouse CD3e-FITC + anti-mouse CD8-PE, anti-mouse CD3e-FITC + anti-mouse CD4-PE, anti-mouse CD3e-FITC + anti-mouse MHC-I-PE or anti-mouse CD3e-FITC + anti-mouse MHC-II-PE (eBioscience, San Diego, CA, USA) for 30 min at 4 °C in the dark. Cell population analyses were conducted with a FACScan flow cytometer with CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA). Lymphocyte specific gating was set according to the forward and side scatter profiles. The percentages of CD4+ and CD8+ T lymphocytes or, MHC-I and MHC-II molecules in mouse splenocytes were determined as previously described [27 (link)].
3
Flow Cytometry Analysis of T Cell Subsets
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Flow cytometry was used to detect the percentages of T cell subsets CD4+ and CD8+ in the splenocytes of mice in the pVAX1-GRA24, pVAX1, PBS, and blank groups. Splenocytes suspensions (5 * 105 cells/ml) were dual-stained with anti-mouse CD3e-FITC+anti-mouse CD8-PE and anti-mouse CD3e-FITC+anti-mouse CD4-PE antibodies (eBioscience), for 30 min at 4°C in the dark. Cell populations were detected using a FACScan flow cytometer and analyzed using CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA).
4
Murine Splenocyte Phenotyping by Flow Cytometry
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The percentages of T cells subsets CD4+ and CD8+, beside MHC-I and MHC-II molecules in the splenocytes of mice in the test groups, rTgEF-1α, adjuvant, PBS, and blank, were determined using flow cytometry technique as described previously (Sasai et al., 2000 (link)). Splenocytes suspensions (1 × 106 cells/ml) were dually stained with anti-mouse CD3e-FITC+anti-mouse CD8-PE, anti-mouse CD3e-FITC+anti- mouse CD4-PE, anti-mouse CD3e-FITC+anti-mouse MHC-I-PE or anti-mouse CD3e-FITC+anti- mouse MHC-II-PE (eBioscience) for 30min at 4°C in the dark. Cell population analysis was conducted by FACScan flow cytometer with CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA). A lymphocyte specific gating was set according to forward and side scatters profiles. The percentages of CD4+ and CD8+ T lymphocytes, MHC-I and MHC-II molecules in mice splenocytes were determined as described by Song et al. (2010) (link).
5
Flow Cytometry Analysis of Mice Splenocytes
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The percentages of T cells subsets CD4+ and CD8+, beside MHC-I and MHC-II molecules in the spleenocytes of mice in the test groups, pTgDPA and pVAX1, PBS and blank, were analyzed using the flow cytometry technique as described by [22 (link)].
Splenocytes suspensions (1 × 106 cells/ml) were dually stained with anti-mouse CD3e-FITC + anti-mouse CD8-PE, anti-mouse CD3e-FITC + anti-mouse CD4-PE, anti-mouse CD3e-FITC + anti-mouse MHC-I-PE or anti-mouse CD3e-FITC + anti- mouse MHC-II-PE (eBioscience) for 30 min at room temperature in the dark. Cell population analysis was conducted by FACScan flow cytometry with CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA). A lymphocyte specific gating was set according to forward and side scatters profiles. The percentages of CD4+ and CD8+ T lymphocytes, MHC-I and MHC-II molecules in mice spleenocytes were determined as described by [23 (link)].
Splenocytes suspensions (1 × 106 cells/ml) were dually stained with anti-mouse CD3e-FITC + anti-mouse CD8-PE, anti-mouse CD3e-FITC + anti-mouse CD4-PE, anti-mouse CD3e-FITC + anti-mouse MHC-I-PE or anti-mouse CD3e-FITC + anti- mouse MHC-II-PE (eBioscience) for 30 min at room temperature in the dark. Cell population analysis was conducted by FACScan flow cytometry with CellQuest software (BD Biosciences, Franklin Lakes, NJ, USA). A lymphocyte specific gating was set according to forward and side scatters profiles. The percentages of CD4+ and CD8+ T lymphocytes, MHC-I and MHC-II molecules in mice spleenocytes were determined as described by [23 (link)].
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