S1P (d18:1), HC-030031, AMG 9810, SEW 2871, CYM 5520, CYM 5541, JTE 013, and TY 52156 were purchased from Cayman Chemical (MI, USA). Ex 26, CYM 50260, and A 971432 were purchased from Tocris Bioscience (MN, USA). Allyl isothiocyanate (AITC) and capsaicin were purchased from MilliporeSigma (MA, USA). S1P stock (10 mM, in DMSO) was used for in vitro experiments and 10 mM S1P stock in H2O filtered by 20 µm nylon filter (VWR International, LCC., PA, USA) was used for in vivo experiments. HC-030031 and AMG 9810 were stocked in DMSO (27.6 mM and 29.0 mM, respectively). Stock solutions were prepared as described. SEW2871 was dissolved in DMSO (50 mM). CYM 5520 and CYM 50260 were dissolved in 10 mM in DMSO. CYM5541, JTE 013, TY 52156, Ex 26, and A 971432 were prepared in 100 mM in DMSO. AITC and capsaicin were dissolved in ethanol (100 mM and 1 mM, respectively). All stocks were stored at −20°C.
Hc 030031
Manufactured by Cayman Chemical
Sourced in United States
HC-030031 is a specialized laboratory equipment used for analytical purposes. It is designed to perform specific functions within the research and development process, without making claims about its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Sew2871, by Cayman Chemical (1 mentions)
Jte 013, by Cayman Chemical (1 mentions)
Amg9810, by Cayman Chemical (1 mentions)
Ex 26, by Bio-Techne (1 mentions)
Cym 5520, by Cayman Chemical (1 mentions)
Cym50260, by Bio-Techne (1 mentions)
A971432, by Bio-Techne (1 mentions)
Ty 52156, by Cayman Chemical (1 mentions)
Cinnamaldehyde, by Merck Group (1 mentions)
6 shogaol, by Cayman Chemical (1 mentions)
Formalin solution, by Merck Group (1 mentions)
Pentobarbital sodium, by Merck Group (1 mentions)
Tween 80, by Avantor (1 mentions)
Fluorescein isothiocyanate (fitc), by Merck Group (1 mentions)
Tween 20, by Avantor (1 mentions)
Allyl isothiocyanate, by Thermo Fisher Scientific (1 mentions)
Histamine dihydrochloride, by Merck Group (1 mentions)
Sb366791, by Cayman Chemical (1 mentions)
Ruthenium red, by Thermo Fisher Scientific (1 mentions)
Capsazepine, by Cayman Chemical (1 mentions)
5 protocols using hc 030031
1
Detailed Compound Preparation for In Vitro and In Vivo Experiments
2
Characterization of TRPA1 Agonist Effects
3
Mouse IgG1 and IL-6 ELISA Protocol
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DINP (>99%), DBP (>99%), FITC, pentobarbital sodium and formalin solution (4%) were bought from Sigma-Aldrich (St. Louis, MO, USA). Tween-20 and Tween-80 were purchased from Amresco (Solon, OH, USA). HC030031 was custom synthesized by Cayman Chemical Company (Ann Arbor, Michigan, USA). ELISA kits (mouse) for total IgG1, IL-6 were obtained from Blue Gene (Shanghai, China) and eBioscience (San Diego, CA, USA), respectively. The protein test kits were provided by Beijing Dingguo Changsheng Biotechnology Co. LTD (Beijing, China).
4
Histamine and TRP Receptor Pharmacology
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Histamine dihydrochloride and capsaicin (TRPV1 agonist) were purchased from Sigma-Aldrich (Steinheim, Germany). The 4-methylHistamine dihydrochloride (4-MH; H2R /H4R agonist), and histamine trifluoromethyl toluidine dimaleate (HTMT; H1R/H2R agonist) were obtained from Tocris Bioscience (Bristol, UK). ST-1006 (H4R agonist) and JNJ-7777120 (H4R antagonist) were synthesized and provided by Prof. Dr. H. Stark (Heinrich-Heine-Universität, Düsseldorf, Germany) [13 (link),14 (link)]. Allylisothiocyanate (AITC; TRPA1 agonist) and ruthenium red (TRP channel inhibitor) were purchased from Acros Organics, Morris Plaines, NJ, USA. Capsazepine (TRPV1 inhibitor), SB366791 (TRPV1 inhibitor) and HC-030031 (TRPA1 inhibitor) were obtained from Cayman Chemicals (Ann Arbor, MI, USA). Diphenhydramine hydrochloride (H1R antagonist) was purchased from West Ward Pharmaceuticals (Eatontown, NJ, USA).
5
Preparing Compounds for In Vitro Experiments
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Drugs for in vitro experiments were dissolved in ethanol and diluted in HBS with a maximum final concentration of ethanol of 0.1%. NDGA, terameprocol, and HC 030031 were purchased from Cayman Chemical (Ann Arbor, MI). Ruthenium red was from Enzo Lifesciences (Farmingdale, NY). Ionomycin was from Ascent Scientific (Avonmouth, UK). CA was purchased from Sigma-Aldrich (Castle Hill, NSW, Australia). All tissue culture reagents were from Sigma-Aldrich, Life Technologies (Mulgrave, Victoria, Australia) or InvivoGen (San Diego, CA).
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