Zno nanogard

ZnO NPs (Lot No.: D28X017, Cat. No. 44898, ZnO NPs, ZnO NanoGard^®) were obtained from Alfa Aesar Co. (Ward Hill, Haverhill, MA, USA); the average particle size of the ZnO NPs in the powder was 67 nm (40–100 nm), and the range of the specific surface area (based on the Brunauer–Emmett–Teller [BET] theory) was 16 m²/g (10–25 m²/g). Horse serum (HS), fetal bovine serum (FBS), penicillin-streptomycin, and Minimal Essential Eagle’s Medium (MEM) were purchased from Gibco Co. (Grand Island, NY, USA); N,N,N′,N′-tetrakis-(2-pyridylmethyl) ethylenediamine (TPEN), LY294002, and rapamycin were purchased from Enzo Life Sciences (Farmingdale, NY, USA); and esculetin, meloxicam, phenidone, N-acetylcysteine (NAC), deferoxamine mesylate, 4′,6′-diamidino-2-phenylindole (DAPI), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). The ZnO NP suspensions were prepared with Millipore water at a concentration of 1 mg/mL. Prior to adding the ZnO NPs to the culture media, each suspension was sonicated for at least 30 min and then vigorously vortexed. Thereafter, the suspension was immediately applied to the cultured astrocytes.

ZnO NPs (Lot No.: D28X017, Cat. No. 44898, ZnO NPs, ZnO NanoGard^®) were purchased from Alfa Aesar Co. (Ward Hill, Haverhill, MA, USA); the average particle size of the ZnO NPs in the powder was 67 nm (40–100 nm), and the range of the specific surface area (based on the Brunauer-Emmett-Teller [BET] theory) was 16 m²/g (10–25 m²/g).