Total RNA was extracted from hCASMCs using an RNeasy Protect Cell Mini Kit (Qiagen, Hilden, Germany), according to the manufacturer's protocol, as described previously 1 , 20 , 21 , 22 , 23 , 24 . After quantifying the isolated RNA using a spectrophotometer, 0.5‐μg aliquots were reverse‐transcribed using a High‐Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). PCR primers were obtained from Nihon Gene Research Laboratories (Sendai, Japan). The primer sequences used to amplify IL‐6, EREG, and AREG mRNA are shown in Table 1 . The real‐time PCR assay was carried out using a SYBR® qPCR kit (KAPA Biosystems, Woburn, MA, USA) in a Thermal Cycler Dice (TaKaRa, Kusatsu, Japan). The mRNA expression level was normalized to that of β‐actin.
Sybr qpcr kit
Manufactured by Roche
Sourced in United States
The SYBR® qPCR kit is a reagent system designed for quantitative real-time PCR (qPCR) analysis. The kit contains SYBR® Green I dye, which binds to double-stranded DNA and emits a fluorescent signal proportional to the amount of amplified DNA. This allows for the real-time monitoring and quantification of DNA targets during the PCR process.
Automatically generated - may contain errors
Lab products found in correlation
Rneasy protect cell mini kit, by Qiagen (1 mentions)
Thermal cycler dice, by Takara Bio (1 mentions)
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Superscript reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
7500 real time pcr system, by Thermo Fisher Scientific (1 mentions)
Rneasy kit, by Qiagen (1 mentions)
2 protocols using sybr qpcr kit
1
RNA Extraction and qPCR Analysis of Cytokine Gene Expression in hCASMCs
2
Gene Expression Analysis by qPCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from cells with RNeasy kit (Qiagen) according to the manufacturer's instruction. Complementary DNA (cDNA) was synthesized with Superscript reverse transcriptase (Invitrogen). Gene expressions were measured by 7500 real-time PCR system (Applied Biosystems) with SYBR qPCR kit (KAPA). Actinb, Gapdh, or Rn18S was used as internal control. The primer sequences are available upon request.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!