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β mercaptoethanol m3148

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β-mercaptoethanol (M3148) is a reducing agent commonly used in biochemical research applications. It functions to break disulfide bonds in proteins, facilitating sample preparation and analysis.

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Lab products found in correlation

Ultrapure water purification system, by Merck Group (2 mentions) Tris glycine electrophoresis buffer, by Bio-Rad (2 mentions) Tris buffered saline with tween 20 sc 362196, by Santa Cruz Biotechnology (2 mentions) Paraformaldehyde 157 4, by Electron Microscopy Sciences (2 mentions) Triton x 100 bp 151, by Thermo Fisher Scientific (1 mentions) Premixed 10 tris glycine sds electrophoresis buffer, by Bio-Rad (1 mentions) Ultrapure water system, by Merck Group (1 mentions)

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β-mercaptoethanol (2521 citations) Mercaptoethanol (160 citations) M3148 (43 citations)

4 protocols using β mercaptoethanol m3148

1

Synthesis and Characterization of BPMAC Compound

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Daunomycin (D8809-1MG), tetramethylethylenediamine
(TEMED, T9281), ammonium persulfate (APS, A3678), β-mercaptoethanol
(M3148), and 30%T, 2.7%C acrylamide/bis-acrylamide (37.5:1) (A3699)
were purchased from Sigma-Aldrich. Triton X-100 (BP-151) was purchased
from ThermoFisher Scientific. Premixed 10× Tris/glycine/SDS electrophoresis
buffer (25 mM Tris, pH 8.3; 192 mM glycine; 0.1% SDS) was purchased
from BioRad. Deionized water (18.2 MΩ) was obtained using an
Ultrapure water system from Millipore. N-[3-[(3-Benzoylphenyl)formamido]propyl]
methacrylamide (BPMAC) was custom synthesized by PharmAgra Laboratories.
Note that the BPMAC used in the previous studies was synthesized in-house17 (link),18 (link) and is a positional isomer (para-form, N-[3-[(4-benzoylphenyl)formamido]propyl]
methacrylamide) of the BPMAC used in the present study.
Kang C.C., Lin J.M., Xu Z., Kumar S, & Herr A.E. (2014). Single-Cell Western Blotting after Whole-Cell Imaging to Assess Cancer Chemotherapeutic Response. Analytical Chemistry, 86(20), 10429-10436.
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2

TEMED-mediated SDS-PAGE Polymerization

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Tetramethylethylenediamine (TEMED, T9281), ammonium persulfate (APS, A3678), β-mercaptoethanol (M3148), and 30%T, 2.7%C acrylamide/bis-acrylamide (37.5:1) (A3699), bovine serum albumin (BSA, A9418) were purchased from Sigma-Aldrich. Triton X-100 (BP-151), phosphate-buffered saline (PBS, ph 7.4, 10010023) and Dulbeccos’s phosphate-buffered saline (PBS, 14190144) were purchased from ThermoFisher Scientific. Premixed 10X tris-glycine EP buffer (25 mM Tris, pH 8.3; 192 mM glycine; 0.1% SDS) was purchased from BioRad. Tris buffered saline with Tween-20 (prepared from 20X TBST, sc-24953, Santa Cruz Biotechnology, Dallas, TX, USA). Deionized water (18.2 MΩ) was obtained using an Ultrapure water system from Millipore. N-[3-[(3-Benzoylphenyl)formamido]propyl] meth- acrylamide (BPMAC) was custom synthesized by Pharm-Agra Laboratories (Brevard, NC, USA).
Rosàs-Canyelles E., Dai T., Li S, & Herr A.E. (2018). Mouse-to-mouse variation in maturation heterogeneity of smooth muscle cells. Lab on a chip, 18(13), 1875-1883.
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3

Biochemical Analysis of Cell Membranes

Cited 2 times
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Hoechst 33342 (B2261), digitonin (D141), Triton X-100 (100×), sodium dodecyl sulfate (SDS, L4509), sodium deoxycholate (D6750), lipopolysaccharides (LPS) from Escherichiacoli (L4524), tetramethylethylenediamine (TEMED, T9281), ammonium persulfate (APS, A3678), β-mercaptoethanol (M3148), and 30%T, 3.3%C acrylamide/bis-acrylamide (29:1; A3574) were purchased from Sigma-Aldrich (St Louis, MO, USA). In addition, 10× tris-glycine electrophoresis buffer (25 mM Tris, 192 mM glycine, pH 8.3 at 1×) was purchased from Bio-Rad (Hercules, CA, USA) and 20× Tris-buffered saline with Tween 20 (sc-362196) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Deionized water (18.2 MΩ) water was obtained from a Millipore Ultrapure water purification system (Billerica, MA, USA). N-[3-[(3-benzoylphenyl)formamido]propyl] meth-acrylamide (BPMAC) was synthesized by Pharm-Agra Laboratories (Brevard, NC, USA)25 (link),26 (link). Paraformaldehyde (157-4) was obtained from Electron Microscopy Services (Hatfield, PA, USA).
Yamauchi K.A, & Herr A.E. (2017). Subcellular western blotting of single cells. Microsystems & Nanoengineering, 3, 16079.
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4

Protein Extraction and Characterization Protocol

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Hoechst 33342 (B2261), digitonin (D141), Triton X-100 (100 × ), sodium dodecyl sulfate (SDS, L4509), sodium deoxycholate (D6750), lipopolysaccharides (LPS) from Escherichia coli (L4524), tetramethylethylenediamine (TEMED, T9281), ammonium persulfate (APS, A3678), β-mercaptoethanol (M3148), and 30%T, 3.3%C acrylamide/bis-acrylamide (29:1; A3574) were purchased from Sigma-Aldrich (St Louis, MO, USA). In addition, 10 × tris-glycine electrophoresis buffer (25 mM Tris, 192 mM glycine, pH 8.3 at 1 × ) was purchased from Bio-Rad (Hercules, CA, USA) and 20 × Tris-buffered saline with Tween 20 (sc-362196) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Deionized water (18.2 MΩ) water was obtained from a Millipore Ultrapure water purification system (Billerica, MA, USA). N-[3-[(3-benzoylphenyl) formamido]propyl] methacrylamide (BPMAC) was synthesized by Pharm-Agra Laboratories (Brevard, NC, USA)25 (link),26 (link). Paraformaldehyde (157-4) was obtained from Electron Microscopy Services (Hatfield, PA, USA).
Yamauchi K.A, & Herr A.E. (2017). Subcellular western blotting of single cells. Microsystems & Nanoengineering, 3, 16079.
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