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Ab9023

Manufactured by Abcam
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Sourced in United Kingdom

Ab9023 is a primary antibody product manufactured by Abcam. It is a monoclonal antibody that binds to a specific target antigen. The core function of this product is to serve as a research tool for the detection and study of the target protein in various biological samples and applications.

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Lab products found in correlation

Ab7754, by Abcam (2 mentions) Ab668, by Abcam (1 mentions) Ab32579, by Abcam (1 mentions)

2 protocols using ab9023

1

Histological Evaluation of Tissue Samples

Cited 1 time
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Biopsies were formalin fixed, and tissue sections (4 µm thick) were cut. Sections were H&E stained, and reviewed by a single histopathologist (SSC) blinded to the diagnosis. Dysplasia and histological severity of inflammation were defined according to standard criteria.26 (link)
34 (link) Immununohistochemistry of K8, K18 and K19 was essentially as described using antibodies to K8, K19 ab9023, ab7754, respectively (Abcam, Cambridge, UK) and K18 (in-house monoclonal antibody). Scoring of keratin for crypt intensity, crypt depth and surface intensity were as previously described.35 (link) Immunohistochemistry (n=48, was performed where formalin-fixed paraffin embedded (FFPE) tissue was adequately well oriented for scoring).
Corfe B.M., Majumdar D., Assadsangabi A., Marsh A.M., Cross S.S., Connolly J.B., Evans C.A, & Lobo A.J. (2015). Inflammation decreases keratin level in ulcerative colitis; inadequate restoration associates with increased risk of colitis-associated cancer. BMJ Open Gastroenterology, 2(1), e000024.
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2

Western Blot Analysis of Keratins

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Buffer exchange, western transfer and immunoblotting were as previously described.4 (link) Primary antibodies were mouse monoclonal: anti-K8 (ab9023); K18 (ab668), K19 (ab7754); K8 phospho-specific rabbit antiphospho S73 antibody (ab32579) and phospho S431 (ab59434) (supplied by Abcam, Cambridge, UK). In-house antibodies raised against acetylated lysine10 residue of K8 (rabbit).33 (link) Densitometric analysis is detailed in online supplementary data, section 7. Immunoblotting was performed for (n=56) patients.
Corfe B.M., Majumdar D., Assadsangabi A., Marsh A.M., Cross S.S., Connolly J.B., Evans C.A, & Lobo A.J. (2015). Inflammation decreases keratin level in ulcerative colitis; inadequate restoration associates with increased risk of colitis-associated cancer. BMJ Open Gastroenterology, 2(1), e000024.
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