rhIL-6R and hIL-6 were purchased from R&D Systems, Inc. (Minneapolis, MN, USA). 2-Mercaptoethanol, Giemsa, dimethyl sulfoxide (DMSO), and MTT were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). 3H-tritiated thymidine and ATPlite kit were purchased from PerkinElmer Inc. (Waltham, MA, USA). Genomic DNA Purification Kit was purchased from Promega Corporation, (Fitchburg, WI, USA).
Rhil 6r
Manufactured by R&D Systems
Sourced in United States
RhIL-6R is a recombinant human Interleukin-6 Receptor protein. It is a soluble form of the Interleukin-6 Receptor that can bind to Interleukin-6, potentially modulating its biological activity.
Automatically generated - may contain errors
Lab products found in correlation
Atplite kit, by PerkinElmer (1 mentions)
Hil 6, by R&D Systems (1 mentions)
Giemsa, by Merck Group (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
2 mercaptoethanol, by Merck Group (1 mentions)
Genomic dna purification kit, by Promega (1 mentions)
Ebm 2 basal medium, by Lonza (1 mentions)
Rhil 6, by R&D Systems (1 mentions)
Rhtnf α, by R&D Systems (1 mentions)
Recombinant human il 17a, by R&D Systems (1 mentions)
Rat tail collagen type 1, by Thermo Fisher Scientific (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
2 protocols using rhil 6r
1
Cytokine-Mediated Cell Proliferation Assay
2
Inflammatory Cytokine Effects on hCMEC/D3 Cells
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Human cerebral microvascular endothelial cells (hCMEC/D3) (Weksler et al., 2013; Weksler et al., 2005) at passages 26-35 were cultured on rat-tail collagen type I-coated tissue culture plates (for gene expression studies) or Transwell® inserts (for assessments of cell monolayer integrity) containing EBM-2 basal medium (Lonza, Walkersville, MD) supplemented with the EGM-2 SingleQuot reagents obtained from the manufacturer. Cells were maintained at 37 °C and 5% CO2 until confluence. Immortalized hCMEC/D3 cell line was kindly provided by Ashley Hayes, F. Hoffmann-La Roche Ltd., Basel, Switzerland. rat-tail collagen type I was obtained from Gibco, Madison, WI.
Recombinant human (rh) IL17A, rhTNF-α, rhIL6 and rhIL6R were purchased from R&D Systems. The experiments were run in 2-fold serial dilutions for each cytokine. Final concentrations used in this study: 50 ng/ml IL-17A ± 1 ng/ml TNF-α or 25 ng/ml IL-6 + 50 ng/ml IL-6R. For gene expression analyses, total RNA was extracted using RNeasy Mini Kit (QIAGEN) after 2 days of treatment with the cytokines.
Recombinant human (rh) IL17A, rhTNF-α, rhIL6 and rhIL6R were purchased from R&D Systems. The experiments were run in 2-fold serial dilutions for each cytokine. Final concentrations used in this study: 50 ng/ml IL-17A ± 1 ng/ml TNF-α or 25 ng/ml IL-6 + 50 ng/ml IL-6R. For gene expression analyses, total RNA was extracted using RNeasy Mini Kit (QIAGEN) after 2 days of treatment with the cytokines.
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