Collagen 1 coated coverslips

Manufactured by BD

Sourced in Uruguay

Collagen I-coated coverslips are a type of lab equipment used in cell culture applications. They provide a substrate coated with collagen type I, which can promote cell attachment and growth for various cell lines.

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Lab products found in correlation

Axio imager, by Zeiss (2 mentions) Alexa488 donkey anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Rat anti mouse cd31 monoclonal antibody, by BD (1 mentions) Fluoview 500, by Olympus (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Alexa568 phalloidin, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal wt1 antibody, by Santa Cruz Biotechnology (1 mentions) Fluoview 500 confocal microscope, by Olympus (1 mentions) Vectashield mounting medium containing dapi, by Vector Laboratories (1 mentions) Brdu labeling reagent, by Thermo Fisher Scientific (1 mentions) Alexa 488 conjugated goat anti mouse secondary antibody, by Thermo Fisher Scientific (1 mentions) Provis ax70 fluorescent microscope, by Olympus (1 mentions)

Spelling variants of this product

Collagen I (161 citations) Collagen-I-coated (3 citations) Collagen coated coverslips (2 citations) Collagen type-I-coated coverslips (2 citations)

5 protocols using collagen 1 coated coverslips

Phage Internalization Assay in Cells

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2 × 10⁵ cells were grown in 6-well tissue culture plates on collagen-I-coated coverslips (BD Biosciences, San Jose, CA) overnight at 37°C in 5% CO₂, and incubated with 10⁸ pfu of T7 phage. The cells were fixed in 4% paraformaldehyde or cold (−20°C) methanol, and stained with antibodies. Cell nuclei were stained with DAPI or Hoechst 33342. Primary antibodies used were rat monoclonal anti-mouse CD31 antibody (BD Biosciences), rabbit anti-NRP-1, mouse anti-NRP-1 (Miltenyi Biotec Inc., Auburn, CA). The secondary antibodies, Alexa594 goat antibodies to mouse, rat, and rabbit immunoglobulins and Alexa488 donkey anti-rabbit antibody were from Invitrogen (Carlsbad, CA). Cells and tissue sections were examined by confocal microscopy (Fluoview 500, Olympus America Inc., Center Valley, PA).

Braun G.B., Sugahara K.N., Yu O.M., Kotamraju V.R., Mölder T., Lowy A.M., Ruoslahti E, & Teesalu T. (2016). UROKINASE-CONTROLLED TUMOR PENETRATING PEPTIDE. Journal of controlled release : official journal of the Controlled Release Society, 232, 188-195.

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Collagen I-mediated Neuropilin-1 Signaling

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Cells grown on collagen I-coated coverslips (BD Biosciences) were treated with 10 μM peptide for 4 hr at 37°C. The cells were fixed, stained with DAPI (Molecular Probes), and viewed with a Fluoview confocal microscope. Some cells were treated with a blocking anti-NRP-1 b1b2 antibody or control IgG for 20 min before and throughout the peptide incubation.

Sugahara K.N., Scodeller P., Braun G.B., de Mendoza T.H., Yamazaki C.M., Kluger M.D., Kitayama J., Alvarez E., Howell S.B., Teesalu T., Ruoslahti E, & Lowy A.M. (2015). A tumor-penetrating peptide enhances circulation-independent targeting of peritoneal carcinomatosis. Journal of controlled release : official journal of the Controlled Release Society, 212, 59-69.

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Immunofluorescence Imaging of Podocytes

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Conditionally immortalized human podocytes were differentiated per established protocols on collagen I–coated coverslips (BD Biosciences) and processed as described previously10 (link). Cells were incubated with mouse monoclonal WT1 antibody (Santa Cruz), mouse monoclonal nephrin antibody (Santa Cruz), or polyclonal rabbit CD2AP (Abcam) overnight at 4 °C. Cells were then washed with ice-cold PBS and secondary Alexa Flora 488 antibody was applied (Invitrogen) at a concentration of 1:1000 for 1 hour at room temperature. Cells were then washed four times with PBS at room temperature before addition of phalloidin alexa-568 (Invitrogen) for one hour at RT followed by two PBS washes and then 4′,6-diamidino-2-phenylindole stain at a concentration of 1:20,000 diluted in PBS. Immunofluorescence imaging was performed using a Carl Zeiss AxioImager and the ZenBlue Bioimaging Software.

Hall G., Lane B., Chryst-Ladd M., Wu G., Lin J.J., Qin X., Hauser E.R, & Gbadegesin R. (2017). Dysregulation of WTI (−KTS) is Associated with the Kidney-Specific Effects of the LMX1B R246Q Mutation. Scientific Reports, 7, 39933.

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Visualizing Tumor-CAF Interactions

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A mixture of tumor cells and CAFs at a 2:1 ratio was grown on collagen I-coated coverslips (BD Biosciences) overnight or until they became confluent. The cells were incubated with 20 μM of FAM-labeled peptides for 4 h at 37 °C. The cells were briefly rinsed with warm PBS, fixed in warm 4% paraformaldehyde, and stained with DAPI (1:1000). The cells were visualized with a Fluoview 500 confocal microscope (Olympus America, Center Valley, PA).

Hurtado de Mendoza T., Mose E.S., Botta G.P., Braun G.B., Kotamraju V.R., French R.P., Suzuki K., Miyamura N., Teesalu T., Ruoslahti E., Lowy A.M, & Sugahara K.N. (2021). Tumor-penetrating therapy for β5 integrin-rich pancreas cancer. Nature Communications, 12, 1541.

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BrdU Labeling of Endothelial Cells

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BMECs were cultured on collagen I-coated coverslips (BD Biosciences, San
Jose, CA). At day 5, the culture medium was removed and replaced with BrdU
labeling reagent (Invitrogen, 1:100) in complete culture medium with VEGF
(10ng/ml) for 12h. Cells were fixed for 30 min in 4% PFA-PBS, rinsed with PBS;
and the chromatin was rendered accessible by a 30 min treatment with 2N
hydrochloric acid (HCl) in 1x PBS. After extensive washing with PBS, cells were
blocked by 2% goat serum with 0.2% Triton X-100-PBS and were then incubated with
anti-BrdU (Invitrogen, 1:200) for 16 h. After extensive washing, cells were
incubated with Alexa 488-conjugated Goat anti mouse secondary antibody
(Invitrogen, 1:500). Nuclei were stained with VECTASHIELD mounting medium
containing DAPI (Vector Laboratories, Inc, Burlingame, CA). Cells were observed
and photographed with an Olympus Provis AX-70 fluorescent microscope (Olympus
Corporation, Tokyo, Japan). The total cell number and the number of cells with
positive Brdu labeling was counted using NIH Image J software.

Tang X., Wang J.J., Wang J., Abboud H.E., Chen Y, & Zhang S.X. (2020). Endothelium-specific Deletion of Nox4 Delays Retinal Vascular Development and Mitigates Pathological Angiogenesis. Angiogenesis, 24(2), 363-377.

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