Cd45ra bv605
The CD45RA BV605 is a fluorochrome-conjugated antibody that specifically binds to the CD45RA antigen, a marker expressed on a subset of T cells. This product is designed for use in flow cytometry applications.
Lab products found in correlation
10 protocols using cd45ra bv605
Comprehensive Immunophenotyping by Flow Cytometry
T Cell Phenotyping and Flow Cytometry
Multi-Dimensional Immune Profiling
Multiparametric Flow Cytometry for Immune Profiling
Surface marker expression on PBMCs was assessed via flow cytometry using a 13-color flow-cytometry panel generated by using the Optimized Multicolor Immunofluorescence Panel-030 (OMIP-030)34 as a reference: CD4 BV510, CD127 AlexaFluor 700, CD8a PE-Cy5, CD3 PerCP-Cy5.5, CD45RA BV605, CD25 PE, CD20 APC-Cy7, CD194 PE-Cy7, CD197 PE/Dazzle594 (Biolegend), CCR10 APC (BD Biosciences), Ki-67 BV650, CD196 BB151, CD183 BV421 (BD Horizon) Fixable Viability Dye APC-Cy7 (eBioscience). Samples were acquired using a Cytoflex S flow cytometer (Beckman Coulter) and then analyzed using the gating strategy summarized in ESI Fig. 5.
Multiparameter T Cell Phenotyping
The following mAbs were used to differentiate T cell subsets: CD3—BV570 (BioLegend), CD4—APC (eBioscience), CD8β—ECD (Beckman Coulter), CD95—BV421 (BioLegend), CD28—PerCp/Cy5.5 (BioLegend), CCR7—FITC (BD Pharmogen), CD45RA—BV605 (BioLegend), CD57—BV570 (BioLegend), IFN-γ—APCe780 (eBioscience). Cells following various combination and incubations as described in our previous study,11 (link)
were analyzed on the BD LSR II instrument using DiVa acquisition (BDIS, Mountain View, CA) and the FlowJo analysis software (TreeStar Inc., Ashland, OR).
Twenty (20) of 37 patients in this study completed the entire cell surface analysis.
Multiparametric Immune Profiling of PBMC
After staining, the cells were washed and fixed using 2% PFA. Acquisition was done on BD FACSCelesta (Becton-Dickenson, San Jose, CA). Forward and side scatters and singlets were used to gate and exclude cellular debris. The flow cytometry results were analyzed using FlowJo™ v10.8 Software (BD Life Sciences, Ashland, OR). The details of the antibodies used are as follows: Fixable Viability stain510, CD4 FITC (clone: RPA-T4) from BD Bioscience (San Jose, CA), IL-21R PE (clone: 2G1-K12), CD8 PerCP (clone: SK1), PD1 APC (clone: EH12.2H7), CXCR5 BV421 (clone: J252D4), CD45RA BV605 (clone: HI100), CD38 BV421 (clone: HB-7), CD16 AF700 (clone: B73.1), CD14 BV650 (clone: M5E2), HLADR BV605 (clone: L243), CD20 PerCP (clone: 2H7), CD27 FITC (clone: M-T271), CD43 APC (clone: CD43-10G7) from BioLegend (San Diego, CA).
Multiparametric Phenotyping of Immune Cells
For the absolute quantification of immune cell subsets, total CD19+, CD3+, CD4+, and CD8+ T cells numbers were determined in 50 μL of the peripheral blood by flow cytometry as described below by means of CytExpress software (Beckman Coulter, USA). Absolute numbers of HBs-specific T cells were determined based on relative frequencies of HBs-specific T cells and absolute numbers of total CD4+ T cells per μL of peripheral blood.
Phenotypic analysis of CAR T cells
Cytomegalovirus-specific CD8+ T cell profiling
Multiparametric Flow Cytometry Profiling
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