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Sodium carbonate

Manufactured by Beijing Chemical Works
Sourced in China

Sodium carbonate is an inorganic compound with the chemical formula Na2CO3. It is a white, crystalline solid that is commonly used as a water softener and in the production of glass, soap, and detergents.

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7 protocols using sodium carbonate

1

Preparation of Amyloid-beta Peptide

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1–42 peptide was synthesized by Science Peptide Biological Technology Co., Ltd. (Shanghai, China). Graphite powder (size less than 30 µm) and 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) were purchased from Sinopharm Chemical Reagent Co., Ltd. Concentrated sulfuric acid, sodium nitrate, potassium permanganate, hydrochloric acid, hydrogen peroxide, sodium hydroxide and sodium carbonate were purchased from Beijing Chemical Plant. MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) was bought from Solarbio Science & Technology Co., Ltd. (Beijing, China). Deionized water used in all experiments was obtained from Milli-Q Integral 3 (Merck Millipore, France).
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2

Fluoride Adsorption Optimization Protocol

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The chemicals used in this study were of analytical grade and did not require further purification. Nitric acid (HNO3), sodium hydroxide (NaOH), sodium fluoride (NaF), sodium nitrate (NaNO3), sodium chloride (NaCl), disodium hydrogen phosphate (Na2HPO4·12H2O), sodium sulfate (Na2SO4) and sodium carbonate (Na2CO3) were purchased from Beijing Chemical Factory. Zirconium oxychloride octahydrate (ZrOCl2·8H2O) was purchased from Tianjin Guangfu Fine Chemical Research Institute. The fluoride concentrations and pH values of the aqueous solutions were measured using a PF-1-C type fluoride ion selective electrode and a pH meter, respectively, which were purchased from Shanghai Yue-Ci Electronic Technology Co., Ltd. The morphologies and elemental compositions of the adsorbents were observed using a scanning electron microscope and energy dispersive spectrometer (ZEISS), and the composition of the functional group of the adsorbent was observed using a Fourier transform infrared spectrometer (IRTracer-100). The fluoride stock solution was prepared by dissolving 2.21 g of NaF in 1000 mL deionized water. In addition, other solutions of fluoride ions with different concentrations were prepared by diluting the stock solution.
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3

Synthetic Procedures for Heterocyclic Compounds

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The bulk of the API of IMH (batch nos. 20180608, 20180918, and 20181026) was produced in the authors’ laboratory. MMS (99%) and EMS (99%) were purchased from Alfa Aesar (Shanghai, China). DDTC (99%) and BDC (98%) were obtained from Aladdin (Shanghai, China). HPLC-grade N,N-dimethylacetamide (DMA), dimethyl sulfoxide (DMSO), and n-hexane (95%) were purchased from Innochem (Beijing, China). HPLC-grade acetonitrile (ACN) was acquired from Fisher (Shanghai, China). Sodium thiophenolate (TPO, 97%) and NaOH were also purchased from Innochem (Beijing, China). Ammonium acetate (98%) was provided by SINOPHARM (Beijing, China). N,N-dimethylformamide (DMF) was supplied from Tong Guang (Beijing, China). Dichloromethane (99.5%) was obtained from Xilong Scientific (Guangdong, China). Triethylamine (99.7%) was purchased from J&K Scientific (Beijing, China), and sodium carbonate (99.8%) was purchased from Beijing Chemical Works (Beijing, China). The purified water was purchased from Wahaha (Hangzhou, China).
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4

Graphite-Based Composite Materials Synthesis

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Sulfanilic acid was purchased from TCI (Shanghai) Development Co., Ltd., (Shanghai, China). Natural graphite flakes were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium carbonate, concentrated hydrochloric acid, sodium borohydride, hydrazine (80%, N2H4·H2O), and sodium nitrite were purchased from Beijing Chemical Works. Polyacrylonitrile (PAN, average Mw 150,000) was purchased from HuBei ChuShengWei Chemistry Co., Ltd., (Wuhan, China).
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5

Comprehensive Analytical Procedure for Assessing Phytochemical Composition

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Acetonitrile 99.9%, n-hexane 95% and methanol 99.9% were purchased from Fisher Scientific (Shanghai, China). The fatty acids methyl ester (FAME) reference standard mixture 37 (standard 47885-U) was purchased from Sigma (St. Louis, MO, USA). Sugars standards [glucose, sorbitol and d(+)-trehalose and myo-inositol], phenolic standards (gallic, 4-hydroxybenzoic, 3,4-dihydroxybenzaldehyde, homogentisic, p-coumaric and o-coumaric acids) were purchased from Sigma (St. Louis, MO, USA) or Sigma-Aldrich Chem (shanghai, China). 1,1-Diphenyl-2-picryl-hydrazyl (DPPH), BHT, 3-(2-pyridyl)-5,6-bis (4-phenyl-sulfonic acid)-1,2,4-triazine (ferrozine), rutin and ascorbic acid were purchased from Sigma Chemical Co. BSTFA + TMCS (99:1) was purchased from Supelco. Folin–Ciocalteu’s phenol reagent was purchased from Beijing Solarbio reagent company (Beijing, China). Ferrous chloride, sodium salicylate, ferrous sulphate, hydrogen peroxide, potassium ferricyanide, trichloroacetic acid, disodium phosphate, sodium dihydrogen phosphate, ferric chloride, sodium carbonate, sodium hydroxide, aluminium nitrate, sodium nitrite, petroleum ether (60–90 °C), ethanol and methanol were all purchased from Beijing chemical plant (Beijing, China). All chemicals were of analytical grade. Water was treated in a Milli-Q water purification system (TGI Pure Water Systems, USA).
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6

Biopolymeric Scaffold Fabrication

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GE (type B, from cow hides, 110–130 Blooms) was purchased from Thermo Scientific (Waltham, MA, USA). HAp was obtained from J&K Scientific (Beijing, China). Glutaraldehyde (25% water solution) was purchased from Shantou Xilong Chemicals (Shantou, China). Sodium carbonate and 1,4-dioxane were obtained from Beijing Chemical Works (Beijing, China). Sodium chloride, potassium phosphate dibasic trihydrate, 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid and calcium chloride (anhydrous) were purchased from Sigma–Aldrich (Saint Louis, MO, USA). Sodium bicarbonate, potassium chloride, magnesium chloride hexahydrate, sodium hydroxide, and sodium sulfate were obtained from Sinopharm Chemical Reagents (Shanghai, China).
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7

Commercial Yeast Strains Evaluation

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Four commercial yeast strains, ES488, CY3079, BV818 and VIC, were chosen because of their steady fermentation characteristics and excellent flavor enhancement according to the manufacturer instructions. The strains ES488 and CY3079 (Saccharomyces cerevisiae) were purchased from Enartis (Tracete, Italy) and Lallemand (Montréal, QC, Canada), respectively. Strains BV818 and VIC (Saccharomyces bayanus) were provided from Angel Yeast Co., Ltd. (Hubei, China). Pectinase and potassium metabisulfite were purchased from Enartis (Tracete, Italy). Water was purified through a Milli-Q purification system (Millipore, Bedford, MA, USA). Folin-Ciocalteu reagent was purchased from Beijing Sulaibao Tech Co., Ltd. (Beijing, China). Sodium hydroxide, sodium chloride, ammonium sulfate, aluminum nitrate, sodium sulfate, sodium carbonate, tartaric acid and ethanol were of analytical grade and purchased from Beijing Chemical Works (Beijing, China). The volatile standards used in this study were purchased from Sigma-Aldrich (St. Louis, MO, USA). These standards had a purity above 95%. The internal standard (4-methyl-2-pentanol) was obtained from Sigma-Aldrich (St. Louis, MO, USA) with a 98% purity.
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