The chemical 2-hydroxy-2-methyl-1-phenyl-1-propanone (IRGACURE 1173) was purchased from Sigma Aldrich (St. Louis, USA). Laponite XLG was purchased from BYK (UK). Glycerin (AR, 99%) and gelatin methacryloyl (GelMA) were obtained from Aladdin (Shanghai, China). N-acryloyl glycinamide (NAGA) was supplied by Zhengzhou Alfa Chemical Co., Ltd. (Zhengzhou, China).
Hydrogels were synthesized through a simple one-pot method. First, dried GelMA (0.5 g) and NAGA (3 g) were successively dispersed into ultrapure water (7 ml) by magnetic stirring for 30 min at 50 °C. Subsequently, Laponite (0.2 g) and glycerol (3 ml) were added to the above suspension under vigorous stirring for another 1 h. Then, 1173 reagent (0.1% of NAGA) was quickly added under stirring for 15 min. The uniform solution was promptly cast into molds. Polymerization of the hydrogel was preceded by UV light irradiation to obtain the NAGA/GelMA/Laponite/glycerol composite hydrogel. In this work, hydrogel was used as an abbreviation of the NAGA/GelMA/Laponite/glycerol hydrogels. The P-sEV concentration was adjusted to 2 mg/mL, and 50 μL of P-sEVs was dropped into the hydrogel and immediately used for wound healing.
Hydrogels were synthesized through a simple one-pot method. First, dried GelMA (0.5 g) and NAGA (3 g) were successively dispersed into ultrapure water (7 ml) by magnetic stirring for 30 min at 50 °C. Subsequently, Laponite (0.2 g) and glycerol (3 ml) were added to the above suspension under vigorous stirring for another 1 h. Then, 1173 reagent (0.1% of NAGA) was quickly added under stirring for 15 min. The uniform solution was promptly cast into molds. Polymerization of the hydrogel was preceded by UV light irradiation to obtain the NAGA/GelMA/Laponite/glycerol composite hydrogel. In this work, hydrogel was used as an abbreviation of the NAGA/GelMA/Laponite/glycerol hydrogels. The P-sEV concentration was adjusted to 2 mg/mL, and 50 μL of P-sEVs was dropped into the hydrogel and immediately used for wound healing.