Alzet model 1007d

Manufactured by Durect

Sourced in United States, Macao

The Alzet Model 1007D is a laboratory device designed for the continuous and controlled delivery of substances to experimental animals. It is a programmable osmotic pump that delivers a predetermined dose of a compound over a specific duration.

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Lab products found in correlation

Ang 2, by Merck Group (3 mentions) Tail cuff method, by Visitech (3 mentions) Bp 98a, by Softron (2 mentions) L name, by Merck Group (1 mentions) Pentobarbital, by Merck Group (1 mentions) A9525, by Merck Group (1 mentions) Tribromoethanol, by Merck Group (1 mentions) A7028, by Beyotime (1 mentions) Wild type c57bl 6j mice, by Jackson ImmunoResearch (1 mentions) Alzet model 1002, by Durect (1 mentions) Angiotensin 2, by Merck Group (1 mentions) C57bl 6j male, by Jackson ImmunoResearch (1 mentions) Apoe mice, by Charles River Laboratories (1 mentions) C57bl 6j mice, by Charles River Laboratories (1 mentions) C57bl 6, by Jackson ImmunoResearch (1 mentions) Ang 2, by Durect (1 mentions) Pentobarbital sodium, by Solarbio (1 mentions)

Spelling variants of this product

Alzet 1007D (24 citations) Model 1007D (9 citations) Alzet micro-osmotic pump model 1007D (2 citations) Alzet Mini-Osmotic Pumps Model 1007D (2 citations) Alzet Micro-Osmotic Pumps (Model 1007D) (2 citations)

15 protocols using alzet model 1007d

Angiotensin II-Induced Hypertension Model

Cited 2 times

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Wild-type (WT) C57BL/6 mice (male, 8–12 weeks) were purchased from Jackson Laboratory (Bar Harbor, ME, USA). The procedures were approved by the Animal Care and Use Committee of Capital Medical University (AEE1-2016-045). All investigations were conformed to the Guide for the Care and Use of Laboratory Animals published by the U.S. National Institutes of Health (NIH Publication No.85-23, revised 1996). The Ang II-induced hypertension model was performed by 14-day subcutaneous infusion of Ang II (490 ng/kg/min; Sigma-Aldrich, St. Louis, MO) or saline using osmotic mini-pumps (Alzet MODEL 1007D; DURECT, Cupertino, CA) as previously described (Wang et al., 2016 (link); Lang et al., 2019 (link)). The systolic blood pressure (SBP) and heart rate (HR) of mice was gauged by a tail-cuff system (SoftronBP-98A; Softron, Tokyo, Japan).
Mice were orally gavaged with vehicle or GA (Sigma-Aldrich) at doses of 5 or 20 mg/kg body weight (BW) daily and randomly subjected to the saline or Ang II treatment. A specific eNOS inhibitor L-NAME (Sigma-Aldrich) was administrated in the drinking water (1 mg/ml) (Boe et al., 2013 (link)). After 2 weeks of Ang II or saline infusion, animals were anaesthetized by intraperitoneal injection of an overdose of pentobarbital (100 mg/kg, Sigma-Aldrich). The aortas were harvested and prepared for further histological and molecular experiments.

Yan X., Zhang Q.Y., Zhang Y.L., Han X., Guo S.B, & Li H.H. (2020). Gallic Acid Attenuates Angiotensin II-Induced Hypertension and Vascular Dysfunction by Inhibiting the Degradation of Endothelial Nitric Oxide Synthase. Frontiers in Pharmacology, 11, 1121.

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Dexamethasone Delivery Using Micro-Osmotic Pumps

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Mice (10–12 weeks old) were anesthetized with isoflurane and a 7-day micro-osmotic pump (Alzet Model 1007D, DURECT Corporation, Cupertino, CA, USA) was implanted subcutaneously between the shoulder blades. DEX is a synthetic glucocorticoid and have a similar long-lasting action to cortisol and corticosterone via glucocorticoid receptor (Mulatero et al., 1997 (link)). The pumps contained DEX sodium phosphate (10, 30 and 100 μg/day, FUJIFILM Wako Pure Chemical Corporation) dissolved in saline. Mice in the control group were given subcutaneous injections of an equal volume of saline. Seven days after implanting the micro-osmotic pumps, the mice were killed by an overdose of isoflurane for collection of brain region.

Kawamura N., Katsuura G., Yamada-Goto N., Novianti E., Inui A, & Asakawa A. (2020). Reduced brain fractalkine-CX3CR1 signaling is involved in the impaired cognition of streptozotocin-treated mice. IBRO Reports, 9, 233-240.

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Ang II-Induced Adiposity Regulation by HPE

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Under anesthesia, we implanted an osmotic pump (Alzet model 1007D, DURECT Corporation, Cupertino, CA) under the dorsal skin of mice for subcutaneous infusion of Ang II (1 μg/kg/min; Sigma-Aldrich, MO) or control saline for 7 days. Mice were caged individually after pump implantation. Day 0 was defined as the start day of the Ang II infusion. The HPE used in this study was hydrolysate of human placenta (Laennec; Japan Bio Products Co., Ltd, Tokyo, Japan), which was administered by intramuscular injection of 3.6 mg/kg once a day for 7 days. Saline administered intramuscularly was used as the control. The first injection of HPE or control saline was administered immediately after osmotic pump implantation. Food intake and body mass were measured every day at around 10:00 am. To calculate adipose tissue weight, we combined the weights of the subcutaneous, epididymal, perirenal and mesenteric adipose tissue. Lean body mass weight was calculated by subtracting the adipose tissue weight from the total body weight.

Yamauchi A., Kamiyoshi A., Sakurai T., Miyazaki H., Hirano E., Lim H.S., Kaku T, & Shindo T. (2019). Placental extract suppresses cardiac hypertrophy and fibrosis in an angiotensin II-induced cachexia model in mice. Heliyon, 5(10), e02655.

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Angiotensin II Induced Hypertension in Mice

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We implanted 12- to 16-week-old male mice with osmotic mini-pumps (Alzet model 1007D; DURECT Corp, Cupertino, CA) containing AngII (A9525 Sigma-Aldrich Corp, St. Louis, MO, USA) or saline (n = 22~26 per group). The delivery rate of AngII was 3.2 mg/kg/day for seven days as in the study of Wang HD et al. [26 (link)]. All surgical procedures in this experiment were performed by aseptic manipulation, and disinfectant solution (10% povidone-iodine solution, Meiji Co., Ltd., Tokyo, Japan) was used. Mice were sedated in an anesthesia box filled with 3.5% sevoflurane. Then, under continuous inhalation of 2.5% to 3.5% sevoflurane, we made a mid-scapular skin incision with surgical scissors and fine forceps to make a small pocket and placed an osmotic mini-pump inside. The wound was sutured with silk thread. 5 mg/kg of meloxicam was injected subcutaneously as an analgesic.

Shiraishi Y., Ishigami N., Kujiraoka T., Sato A., Fujita M., Ido Y, & Adachi T. (2021). Deletion of Superoxide Dismutase 1 Blunted Inflammatory Aortic Remodeling in Hypertensive Mice under Angiotensin II Infusion. Antioxidants, 10(3), 471.

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Angiotensin II-Induced Heart Injury Model

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Four groups of forty wild-type (WT) C57BL/6 male mice (8 weeks old) were used. Using osmotic mini-pumps (Alzet Model 1007D or 1002, DURECT), the mice were infused for 7 or 14 days with normal saline or Ang II (1,000 ng/kg/min, Aladdin) (Bai et al., 2022 (link)). The mice were given an oral gavage of FO (300 mg/kg/day) or PBS (internal control) the day before surgery, followed by a 14-day infusion of Ang II or saline (Yu et al., 2020 (link)). All mice received an intraperitoneal injection of 2.5% tribromoethanol (0.02 mL/g, Sigma‒Aldrich) to induce anesthesia after receiving therapy for 14 days. The hearts were removed and used for future research.
The animal experimental procedures were approved by the Animal Experimental Ethics Committee of Dalian Medical University, and extensive efforts were made to minimize the distress of the included animals.

Wang S., Bai J., Che Y., Qu W, & Li J. (2023). Fucoidan inhibits apoptosis and improves cardiac remodeling by inhibiting p53 transcriptional activation through USP22/Sirt 1. Frontiers in Pharmacology, 14, 1164333.

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Pressure-Overload-Induced Cardiac Hypertrophy

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Eight‐week‐old male C57BL/6 mice were subjected to thoracic aorta constriction (TAC) or sham operation as described previously (Okada et al., 2004). Pressure‐overload‐induced cardiac hypertrophy and heart failure was created by TAC for 2 weeks. C57BL/6 mice were continuously infused with isoproterenol hydrochloride (15 mg kg⁻¹ day⁻¹; Sigma‐Aldrich China Inc.) over a period of 14 days with implanted minipumps. Mini‐osmotic pumps (Alzet model 1007D and 1002; DURECT Corp., Cupertino, CA, USA) were implanted as described previously (Nienaber et al., 2003). Mice were anesthetized with pentobarbital sodium at a dose of 80 mg kg⁻¹ body weight intraperitoneally. Animals were euthanized via an anesthetic overdose (200 mg kg⁻¹ of pentobarbital sodium delivered by intraperitoneal injection). All animal studies were approved by the Animal Research Committee of Tongji College and were carried out according to the guidelines of the National Institutes of Health (NIH).

Duan Q., Ni L., Wang P., Chen C., Yang L., Ma B., Gong W., Cai Z., Zou M, & Wang D.W. (2016). Deregulation of XBP1 expression contributes to myocardial vascular endothelial growth factor‐A expression and angiogenesis during cardiac hypertrophy in vivo. Aging Cell, 15(4), 625-633.

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Angiotensin II-Induced Vascular Inflammation

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C57BL/6 J wild-type (WT) mice were purchased from the Jackson Laboratory (Sacramento, CA). Male mice (8 weeks old; n = 6 animals per group) were infused with saline or Ang II at a dose of 1000 ng/kg/min (Aladdin, CA) with an osmotic minipump (Alzet MODEL 1007D for 7 days; Alzet MODEL 1002 for 14 days; DURECT, Cupertino, CA) as previously described [3] . A control IgG (A7028, Beyotime, CA) or anti-ICAM-1 neutralizing antibody (BE0020-1, Bio X Cell, USA) was administered every 2 days beginning 1 day before the operation to block soluble ICAM-1 based on our preliminary results and previous publications [16] . The ICAM-1 neutralizing antibody was administrated by tail intravenous injection (IV.). All mice were maintained under specific pathogen-free conditions and provided free access to a normal diet (0.3% sodium chloride). After the study period, the mice were anesthetized, and the serum was collected and aortic tissues were removed and prepared for further molecular and histological examinations. All procedures were approved by the Institutional Animal Care and Use Committee of Dalian Medical University and conformed to the US National Institute of Health's Guide for the Care and Use of Laboratory Animals.

Lang P.P., Bai J., Zhang Y.L., Yang X.L., Xia Y.L., Lin Q.Y, & Li H.H. (2020). Blockade of intercellular adhesion molecule-1 prevents angiotensin II-induced hypertension and vascular dysfunction. Laboratory investigation; a journal of technical methods and pathology, 100(3).

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Cardiac Hypertrophy Induction in Mice

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C57BL/6 mice (male, 4-5 weeks) were purchased from Beijing Vital River Laboratory (Beijing, China). Mice were housed in individually ventilated cages and fed with diet and water ad libitum. Methionine (Met) diet used in this study contains 2% wt/wt Met [13 (link), 14 (link)], while FA-supplemented Met diet contains additional 0.006% wt/wt FA [15 (link), 16 (link)]. Cardiac hypertrophy in mice was induced by subcutaneous AngII infusion at a dose of 1000 ng/kg/min using osmotic mini-pumps (Alzet Model 1007D; Durect Corp, Cupertino, CA, USA) for three weeks [17 (link)]. The housing, care, and experimental procedures were performed in accordance with the Guide for the Care and Use of Laboratory Animals published by the U.S. National Institutes of Health (NIH Publication No. 85-23, revised 1996) and approved by the Animal Care and Use Committee of Dalian Medical University (AEE20029).

Deng Y., Li Z., An X., Fan R., Wang Y., Li J., Yang X., Liao J, & Xia Y. (2022). Hyperhomocysteinemia Promotes Cardiac Hypertrophy in Hypertension. Oxidative Medicine and Cellular Longevity, 2022, 1486157.

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Tumor Growth Inhibition by YM155

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SW480 cells (2*10⁶ cells) were injected into the flanks of six-week-old SCID mice and allowed to reach a tumor volume of >100 mm³ (length × width² × 0.5). Mice were randomized into groups (n = 6) to receive s.c. saline control or YM155 (10 mg/kg*day) administered as a 7-day continuous infusion using an implanted microosmotic pump (Alzet model 1007D, Durect). YM155 was dissolved and diluted in saline immediately prior to administration. Statistically significant differences were determined using Student’s t test. P < 0.05 was chosen as the threshold for statistical significance. Mice were housed in the pathogen-free animal facility of the Spanish National Cancer Research Centre (CNIO, Madrid) following the animal care standards of the institution. All animal protocols were approved by the ISCIII committee (Madrid) for animal care and research.

Winter G.E., Radic B., Mayor-Ruiz C., Blomen V.A., Trefzer C., Kandasamy R.K., Huber K.V., Gridling M., Chen D., Klampfl T., Kralovics R., Kubicek S., Fernandez-Capetillo O., Brummelkamp T.R, & Superti-Furga G. (2014). The solute carrier SLC35F2 enables YM155-mediated DNA damage toxicity. Nature chemical biology, 10(9), 768-773.

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Angiotensin II-Induced Hypertension Model

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To induce hypertension, osmotic minipumps (Alzet Model 1007D; DURECT, Cupertino, CA, USA) filled with Ang II or acetic acid saline were placed subcutaneously to deliver Ang II at a concentration of 1500 ng/kg /min as described previously.^{9 (link), 22 (link)} Systolic blood pressure was measured at the indicated day using a computerized mouse tail cuff system (BP98A; Softron, Tokyo, Japan) as described elsewhere.^{21 (link), 23 (link)} Cardiac echocardiography was performed using the Vevo 2100 high-resolution microimaging system (VisualSonic, Toronto, ON, Canada) as described previously.^{2 (link)}

Li T.T., Jia L.X., Zhang W.M., Li X.Y., Zhang J., Li Y.L., Li H.H., Qi Y.F, & Du J. (2016). Endoplasmic reticulum stress in bone marrow-derived cells prevents acute cardiac inflammation and injury in response to angiotensin II. Cell Death & Disease, 7(6), e2258-.

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