Dialyzed fcs

Manufactured by Thermo Fisher Scientific

Sourced in United States

Dialyzed FCS is a cell culture supplement derived from fetal bovine serum. It is processed to remove low molecular weight components, including ions, metabolites, and other small molecules. The resulting product is a concentrated source of proteins, growth factors, and other macromolecules essential for cell growth and proliferation in cell culture applications.

Automatically generated - may contain errors

Lab products found in correlation

7 protocols using dialyzed fcs

Evaluating HoPan Modulation of HEK293 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HEK293 cells were maintained as described previously²⁹. For HoPan treatment, cells were cultured in custom-made dMEM without vitamin B5 (Thermo Scientific) and supplemented with dialyzed FCS (Thermo Scientific). P-PantSH or P-PantSAc was added to the cells for a final concentration of 25 µM for 4 days in the presence of HoPan at a final concentration of 0.5 mM. Four days after the start of treatment the cells were counted.

Di Meo I., Colombelli C., Srinivasan B., de Villiers M., Hamada J., Jeong S.Y., Fox R., Woltjer R.L., Tepper P.G., Lahaye L.L., Rizzetto E., Harrs C.H., de Boer T., van der Zwaag M., Jenko B., Čusak A., Pahor J., Kosec G., Grzeschik N.A., Hayflick S.J., Tiranti V, & Sibon O.C. (2017). Acetyl-4′-phosphopantetheine is stable in serum and prevents phenotypes induced by pantothenate kinase deficiency. Scientific Reports, 7, 11260.

+ Open protocol

+ Expand

Cell Culture Protocols for Receptor Binding Studies

Check if the same lab product or an alternative is used in the 5 most similar protocols

Raji, Ramos, SK-BR-3 (DSMZ—German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany) and baby hamster kidney (BHK)-21 cells (American Type Culture Collection, ATCC, Manassas, VA, USA) were kept in RPMI 1640 Glutamax-I medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal calf serum (FCS; Thermo Fisher Scientific, Waltham, MA, USA), 100 U/mL penicillin and 100 µg/mL streptomycin (Thermo Fisher Scientific; R10+ medium). BHK-21 cells that were co-transfected with plasmids encoding the FcεRI γ chain and either human FcγRIIIA 158F (BHK-CD16-158F) or FcγRIIIA 158V (BHK-CD16-158V) were cultured as described [38 (link)]. CHO glycosylation mutant Lec13 cells [39 (link),40 (link)] were maintained in MEM alpha medium with nucleosides (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% dialyzed FCS (Thermo Fisher Scientific, Waltham, MA, USA) and penicillin (100 U/mL)/streptomycin (100 µg/mL). For culturing CHO-K1 and Lec13 cells transfected with antibody expression vectors, hygromycin B (Thermo Fisher Scientific, Waltham, MA, USA) was added to a concentration of 500 μg/mL. CHO cells stably transfected with a plasmid coding for the cDNA of human CD19 (Origene Technologies Inc., Rockville, MD, USA) were generated using standard procedures (Peipp, unpublished).

Roßkopf S., Eichholz K.M., Winterberg D., Diemer K.J., Lutz S., Münnich I.A., Klausz K., Rösner T., Valerius T., Schewe D.M., Humpe A., Gramatzki M., Peipp M, & Kellner C. (2020). Enhancing CDC and ADCC of CD19 Antibodies by Combining Fc Protein-Engineering with Fc Glyco-Engineering. Antibodies, 9(4), 63.

+ Open protocol

+ Expand

Culturing Human Burkitt Lymphoma B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human Burkitt lymphoma B cell-line Ramos was obtained from ATCC, Ramos (RA 1) (ATCC ® CRL-1596 ™ ). Ramos cells were cultured in RPMI 1640 medium (Gibco), supplemented with 5% FCS (Biochrom), 10 units/mL penicillin/streptomycin (Gibco), 20 mM HEPES (Gibco), and 50 mM β-mercaptoethanol (Sigma) at 37°C with 5% CO2. For glucose versus galactose metabolism analysis, Ramos B cells were cultured in RPMI 1640 medium without glucose (Gibco), supplemented with 11 mM sterile filtered glucose or galactose, respectively, 5% dialyzed FCS (Thermo Fisher), 10 units / mL penicillin / streptomycin (Gibco), 20 mM HEPES (Gibco), and 50 mM β-mercaptoethanol (Sigma). Cells were cultured at 37°C with 5% CO2.

Kläsener K., Jellusova J., Andrieux G., Salzer U., Boehler C., Steiner S.N., Albinus J.B., Cavallari M., Suess B., Boerries M., Voll R., Wollscheid B., & Reth M. (2020). CD20 as a gatekeeper of the resting stage of human B cells.

+ Open protocol

+ Expand

Tumor and Lung Explant Culture

Check if the same lab product or an alternative is used in the 5 most similar protocols

After removal and dissection, tumors and lung explants were cultured in DMEM (Gibco, Waltham, MA) supplemented with different concentrations of labeled or unlabeled glucose, 1 mM glutamine (Sigma-Aldrich, St. Louis, MO, USA), 100 U/ml penicillin and 100 µg/ml streptomycin (Gibco), with or without dialyzed fetal calf serum (FCS). For culture under “nonstarvation” conditions, media were supplemented with 5 mM glucose (Sigma) or [¹³C₆]glucose (Cambridge Isotope Laboratories, Tewksbury, MA, USA) and 10% dialyzed FCS (Gibco). For culture under “starvation” conditions, media were supplemented with 1 mM glucose (Sigma) or [¹³C₆]glucose (Cambridge Isotope Laboratories) without FCS. Media were replaced every 24 h.

Lesko J., Triebl A., Stacher-Priehse E., Fink-Neuböck N., Lindenmann J., Smolle-Jüttner F.M., Köfeler H.C., Hrzenjak A., Olschewski H, & Leithner K. (2021). Phospholipid dynamics in ex vivo lung cancer and normal lung explants. Experimental & Molecular Medicine, 53(1), 81-90.

+ Open protocol

+ Expand

SILAC Labeling of Virus-Infected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

HFF1 cells were cultured in SILAC DMEM (PAA, Österreich, Austria), 10% dialyzed FCS (Gibco, Loughborough, UK), 0.280 mM arginine, 0.398 mM lysine, 0.5 mM proline, 10 mM HEPES, 2 mM l-glutamine, 100 IU penicillin and 100 μg/mL streptomycin for >5 cell doublings to ensure complete incorporation of the labeled amino acids. Arginine to proline conversion was not observed under these labeling conditions. The SILAC light medium (L) was supplemented with Arg ¹²C₆¹⁴N₄ and Lys ¹²C₆¹⁴N₂ (Sigma, Zwijndrecht, The Netherlands), the SILAC heavy medium (H) was supplemented with Arg ¹³C₆¹⁵N₄ and Lys ¹³C₆¹⁵N₂ (Cambridge Isotope Laboratories, Cambridge, MA, USA).
HFF1 cells were seeded in six-well plates and grown to a 70–80% confluence. The cultures were rinsed twice with PBS and the cells were incubated with either CHIKV or ZIKV at MOI of 8 for 2 or 1.5 h, respectively, at 37 °C while gently agitating the plates. Then, the inoculum was removed, the cells were washed three times with PBS and SILAC DMEM supplemented with 15% FCS was added to each well after which the plates were incubated at 37 °C and 5% CO₂. Infected and mock-infected cells were lysed at 48 hpi in 4% SDS, 0.1 M Tris pH 7.6.

Wichit S., Hamel R., Zanzoni A., Diop F., Cribier A., Talignani L., Diack A., Ferraris P., Liegeois F., Urbach S., Ekchariyawat P., Merits A., Yssel H., Benkirane M, & Missé D. (2019). SAMHD1 Enhances Chikungunya and Zika Virus Replication in Human Skin Fibroblasts. International Journal of Molecular Sciences, 20(7), 1695.

+ Open protocol

+ Expand

Glucose Uptake Assay in Splenocytes and NK Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

3x10⁶ splenocytes or 0.5x10⁶ cultured NK cells were washed and incubated at 37 °C for 15 min in glucose-free media supplemented with 10% dialyzed FCS (Fisher), 2 mM Glutamine (Life technologies), 1 mM Sodium Pyruvate (Gibco), 1x concentration of MEM Vitamin Cocktail (Life technologies), 1 × concentration of Selenium/Insulin Cocktail (Life technologies), 50 μM β-mercaptoethanol (Sigma) and 1% Penicillin/Streptomycin (Life technologies). Splenocytes and cultured NK cells were then incubated at 37°C for a further 2 hours or 1 hour, respectively, in supplemented glucose-free media containing the fluorescently labelled glucose analogue 2-NBDG (Life technologies) at a final concentration of 50 μM before analysis using flow cytometry.

Donnelly R.P., Loftus R.M., Keating S.E., Liou K.T., Biron C.A., Gardiner C.M, & Finlay D.K. (2014). mTORC1-dependent metabolic reprogramming is a prerequisite for Natural Killer cell effector function. Journal of immunology (Baltimore, Md. : 1950), 193(9), 4477-4484.

+ Open protocol

+ Expand

Cytokine-Driven Expansion and Metabolic Modulation of NK Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Splenocytes were isolated and cultured in IL-15 (25 ng/ml, Peprotech) at 37 °C for 5 days. On day 5, the cells were supplemented with IL-15 (25 ng/ml) and cultured for a further 2 days. On day 7, cultured NK cells were stimulated for 18 hours with IL-2 (20 ng/ml, NCI preclinical repository) and/or IL-12 (10 ng/ml, Miltenyi Biotech) cytokines. Low dose IL15 (5 ng/ml) was added as a survival factor to unstimulated cultures or those stimulated with IL12 alone. Experiments were carried out in the presence or absence of 2-deoxyglucose (2DG, Sigma), rapamycin (20 nM, Fisher) and/or oligomycin (2 μM, Sigma) inhibitors. NK cells were MACS purified using a NK isolation kit (Miltenyi Biotech) from day 7 cultures for biochemical analyses. Where indicated, NK cells were cultured in glucose-free medium supplemented with 10% dialyzed FCS (Fisher), 2 mM Glutamine (Invitrogen/Biosciences), 1 mM Sodium Pyruvate (Gibco), 1x concentration of MEM Vitamin Cocktail (Invitrogen/Biosciences), 1x concentration of selenium/insulin/transferrin Cocktail (Invitrogen/Biosciences), 50 μM β-mercaptoethanol (Sigma) and 1% Penicillin/Streptomycin (Invitrogen/Biosciences) and with either glucose (10 mM) or galactose (10 mM).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!