Rabbit anti caspase 3 9662

Western blotting was performed according to a previous report [28 (link)]. The Rabbit anti-VEGF (19003-1-AP), Rabbit anti-FLK1 (26415-1-AP), Rabbit anti-NFDUFS4 (15849-1-AP), Rabbit anti-alpha-SYN (10842-1-AP), Rabbit anti-TH (25859-1-AP), and Rabbit anti-GAPDH (10494-1-AP) primary antibodies were purchased from Proteintech (Wuhan, China). The Rabbit anti-P38MAPK (5140), Rabbit anti-ERK1/2 (4370), Rabbit anti-p-ERK1/2 (4695), Rabbit anti-SMAD3 (9523), Rabbit anti-p-SMAD3 (C25A9), Rabbit anti-caspase3 (9662) and Rabbit anti-ICAM1(4915) primary antibodies were purchased from Cell Signaling Technology (Danvers, USA). The FITC-labeled anti-Rabbit secondary antibodies were purchased from Cell Signaling Technology (Danvers, USA).

Proteins were extracted from cells using RIPA buffer (Nacalai Tesque, Kyoto, Japan) containing cOmplete ™ Protease Inhibitor Cocktail (Sigma-Aldrich, St. Louis, MO, USA) and the protein concentration was adjusted to 1 mg/mL. Immunoblotting was performed as previously described [16 (link)]. The primary antibodies used in the experiments were as follows: rabbit anti-PAFR, # bs-14730R-A750 (Bioss. Inc., Woburn, MA, USA), 1:200; rabbit anti-Erk1/2 # 9102 (Cell Signaling Technology, Beverly, MA, USA), 1:1000; rabbit anti-p-Erk1/2 # 4370 (Cell Signaling Technology), 1:1000; rabbit anti-Akt # 4691 (Cell Signaling Technology), 1:1000; rabbit anti-p-Akt #4060 (Cell Signaling Technology), 1:1000; rabbit anti-Caspase-3 #9662 (Cell Signaling Technology), 1:1000; rabbit anti-Cleaved caspase-3 #9661 (Cell Signaling Technology), 1:1000; and mouse anti-α tubulin # sc-5286 (Santa Cruz Biotechnology, Shanghai, China).