Myocardial injury in vivo was assessed by the activities of AST, LDH, CK and CK‐MB release in plasma using AST, LDH, CK and CK‐MB assay kits, respectively (Nanjing Jiancheng Bioengineering Institute, China). In brief, blood samples were obtained from the abdominal aorta and centrifuged at 2800 g for 15 minutes at 4°C to separate the plasma. Approximately 2.0 mL of plasma from each rat was collected. The plasma levels of AST, LDH, CK and CK‐MB were measured according to the protocol of Zhang et al.18 Three independent experiments were performed.
Ck mb assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The CK-MB assay kit is a laboratory test used to measure the levels of creatine kinase-MB (CK-MB) in the blood. CK-MB is an enzyme found primarily in the heart muscle and is released into the bloodstream when the heart muscle is damaged, such as during a heart attack. The assay kit provides a method for quantitative determination of CK-MB concentration in human serum or plasma samples.
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Lab products found in correlation
Ldh assay kit, by Nanjing Jiancheng (5 mentions)
Troponin assay kit, by Nanjing Jiancheng (1 mentions)
Ultramicro microporous plate spectrophotometer, by Agilent Technologies (1 mentions)
Mouse ldh elisa kit, by Cusabio (1 mentions)
Mouse tnf α elisa kit, by Thermo Fisher Scientific (1 mentions)
Mouse il 6 elisa kit, by Abcam (1 mentions)
Mda assay kit, by Nanjing Jiancheng (1 mentions)
Gsh assay kit, by Nanjing Jiancheng (1 mentions)
10 protocols using ck mb assay kit
1
Cardiac Injury Biomarker Quantification
2
Myocardial Enzyme Levels in Cardiomyocytes
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After the cardiomyocytes had been subjected to the HR treatment, the levels of myocardial enzymes, including lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB), were notably increased. The levels of LDH and CK-MB in the culture medium and coronary effluent were measured using LDH and CK-MB assay kits, respectively (provided by Jiancheng Bioengineering Institute, Nanjing, China), following the operation guideline.
3
Evaluating Myocardial Injury Markers
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Lactate dehydrogenase (LDH) and creatine kinase-myocardial band (CK-MB) are known as myocardial injury markers (23 (link)). Hence, the levels of LDH and CK-MB in the culture medium were measured to evaluate the extent of myocardial injury using LDH and CK-MB assay kits, respectively (provided by Jiancheng Bioengineering Institute, Nanjing, China), following the manufacturer's instructions.
4
Quantifying Cardiac Enzyme Levels
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Lactate dehydrogenase (LDH) and creatine kinase isoenzyme (CK-MB) were measured using the LDH (Jiancheng, Nanjing, China) and CK-MB assay kits (Jiancheng, Nanjing, China). An appropriate amount of serum from centrifuged abdominal aortic blood was used to detect changes in the LDH and CK-MB levels. The concentrations of both were calculated using a the standard curve.
5
Detecting Myocardial Damage Biomarkers
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When cardiomyocytes suffer from acute damage, lactate dehydrogenase (LDH) and creatine kinase‐MB (CK‐MB) will release into the culture medium. The activities of LDH and CK‐MB were detected using the LDH Assay Kit and CK‐MB Assay Kit (Jiancheng Biotech Co., Ltd) following to the manufacturer's instructions.
6
Cardiac Function Evaluation in Mice
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We centrifuged the blood at 3000 rpm until it strati ed. The serum was collected for biological testing.
The level of LDH and CK-MB were separately measured by LDH assay kit and CK-MB assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). To compare the cardiac function of mice in each group, we used transthoracic echocardiography to evaluate and analyze the cardiac function data of mice in each group.
The level of LDH and CK-MB were separately measured by LDH assay kit and CK-MB assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). To compare the cardiac function of mice in each group, we used transthoracic echocardiography to evaluate and analyze the cardiac function data of mice in each group.
7
Assessing Cardiomyocyte Injury Markers
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When cardiomyocytes suffer from acute damage, lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB) and Troponin (cTnI) will release into the culture medium. The activities of LDH and CK-MB, as well as the concentration of cTnI were detected using the LDH Assay Kit, CK-MB Assay Kit and Troponin Assay Kit (Jiancheng Biotech Co., Ltd, Nanjing, China) following to the manufacturer’s instructions.
8
Cardiomyocyte Enzyme Release Assay
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Lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) released from cardiomyocytes into the culture medium were determined using the LDH Assay Kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and CK-MB Assay Kit (Nanjing Jiancheng Bioengineering Institute), respectively, according to the manufacturer’s protocols. Absorbance was detected at 450 nm using the Ultramicro Microporous Plate Spectrophotometer (BioTek).
9
Quantifying Cell Injury via LDH and CK-MB
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Lactate dehydrogenase (LDH) and creatine kinase MB isoenzyme (CK-MB) activity of cell medium was used to determine the injury to H9c2 cells using a commercial LDH assay kit (Jiancheng Bioengineering Institute, Nanjing, China) and CK-MB assay kit (Jiancheng Bioengineering Institute), according to the manufacturer’s instructions.
10
Cardiac Biomarker and Oxidative Stress Profiling
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Heart tissues were homogenized in iced PBS, and homogenates were centrifuged at 4800 g for 20 minutes to isolate the supernatant. Myocardial lactate dehydrogenase (LDH) and creatine kinase- (CK-) MB were detected by using the commercial kits. The mouse LDH ELISA kit was provided by CUSABIO (#CSB-E17733m, Wuhan, China). The CK-MB assay kit (#A032-1-1) was obtained from the Nanjing Jiancheng Biological Engineering Research Institute.
The levels of glutathione (GSH), malondialdehyde (MDA), 3-nitrotyrosine (3-NT), protein carbonyl, total SOD activity, tumor necrosis factor-α (TNF-α) level, and interleukin- (IL-) 6 level were determined using commercial kits based on the manufacturer's instructions. GSH assay kit (#A061), MDA assay kit (A003-1-2), and the total SOD activity (A001-3-2) were provided by the Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). 3-NT competitive ELISA (#ab113848) was obtained from Abcam. The protein carbonyl ELISA kit was provided by Abnova (#KA6397, Taipei, Taiwan). The TNF-α Mouse ELISA kit (#BMS607-3TEN) was provided by Invitrogen, and the IL-6 (mouse) ELISA kit was provided by Biovision (#K4795). All assays were performed in triplicate.
The levels of glutathione (GSH), malondialdehyde (MDA), 3-nitrotyrosine (3-NT), protein carbonyl, total SOD activity, tumor necrosis factor-α (TNF-α) level, and interleukin- (IL-) 6 level were determined using commercial kits based on the manufacturer's instructions. GSH assay kit (#A061), MDA assay kit (A003-1-2), and the total SOD activity (A001-3-2) were provided by the Nanjing Jiancheng Institute of Biotechnology (Nanjing, China). 3-NT competitive ELISA (#ab113848) was obtained from Abcam. The protein carbonyl ELISA kit was provided by Abnova (#KA6397, Taipei, Taiwan). The TNF-α Mouse ELISA kit (#BMS607-3TEN) was provided by Invitrogen, and the IL-6 (mouse) ELISA kit was provided by Biovision (#K4795). All assays were performed in triplicate.
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