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Sourcing Chemicals for Fungal BGL1 Cloning

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Salicin was bought from Sigma-Aldrich (St. Louis, MO, USA). Furfural and 5-hydroxymethyl Furfural (5-HMF) were purchased from Acros Organics (NJ, USA). Vanillin, 4-hydroxybenzaldehyde, sodium formate, sodium acetate, sodium hydroxide, KCl and NaCl were purchased from Sinopharm Chemical Reagents (Shanghai, China). Formic acid, acetic acid and levulinic acid were purchased from local chemical reagent companies in Nanchang, China. The Glucose Assay Kit and zeocin were bought from Robio, China, and Invitrogen, USA, respectively.
P. oxalicum 16 (PO16) for BGL1 gene cloning was isolated from the local soil of Nanchang [23 (link)]. Escherichia coliTOP 10 was purchased from Takara, Japan. pGAPZαA for constitutive expression of P. pastoris GS115 was purchased from Invitrogen, USA.
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2

Colorimetric Detection of Cobalt Ions

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1,3,5-Benzene tricarboxaldehyde (Bt) (Jilin Chinese Academy of Sciences – Yanshen Technology Co., Ltd., Jilin, China), p-phenylenediamine (PD) (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), 1,4-dioxane (Xilong Scientific Co., Ltd., Shantou, China), 3 mol L−1 CH3COOH (Xilong Scientific Co., Ltd., Shantou, China), N,N-dimethylformamide (DMF) (Xilong Scientific Co., Ltd., Shantou, China), tetrahydrofuran (THF) (Xilong Scientific Co., Ltd., Shantou, China), 1% trisodium citrate (Xilong Scientific Co., Ltd., Shantou, China), sodium borohydride (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), 10 μmol L−1 Victoria blue 4R (VB4R) (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), 0.1 mol L−1 NaCl (Xilong Scientific Co., Ltd., Shantou, China), 0.01 mol L−1 HCl (Xilong Scientific Co., Ltd., Shantou, China), 0.1 mol L−1 sodium formate (Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), 100 nmol L−1 Co(NO3)2·6H2O (Xilong Scientific Co., Ltd., Shantou, China), 1% HAuCl4 (Shanghai Macklin Biochemical Co., Ltd., Shanghai, China), and 0.1 μmol L−1 Apt with a sequence of 5′-3′ GGT AAT ACG ACT CAC TAA GGG AGA TAC CAG CTT ATT CAA TTT TAC AGA ACA CCA ACG TCG CTC GGG TAC TTC TTC ATC GAG ATA GTA AGT GCA ACT T (Shanghai Sangon Biotech Co., Ltd., Shanghai, China) were used. All reagents were of analytical grade, and the water was double distilled.
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3

Antibody-Based Analysis of Apoptosis Pathways

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Polyclonal rabbit anti-mouse antibodies raised against Bax and Bcl-2 were obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA; cat. nos. sc-6236 and sc-492). Antibodies against cleaved caspase-9 (cat. no. 7237), cleaved caspase-3 (cat. no. 9579), c-Jun N-terminal kinase (JNK; cat. no. 9251), phosphorylated (p)-JNK (cat. no. 9252) and microtubule-associated protein 1A/1B-light chain 3 (LC3; cat. no. 2775) were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). A mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase was purchased from Kangchen Bio-Tech Co., Ltd. (Shanghai, China; cat. no. kc-564). All primary antibodies were diluted 1:1,000. The secondary antibodies (goat anti-rabbit; cat. no. 31210; 1:5,000) were obtained from Thermo Fisher Scientific, Inc. (Waltham, MA, USA). An enhanced chemiluminescence (ECL)-Plus kit was purchased from Beyotime Institute of Biotechnology (Nantong, China). An annexin V-FLUOS Staining kit was purchased from Roche Diagnostics (Mannheim, Germany). Sodium formate was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Hoechst 33342 stain, propidium iodide (PI), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), monodansylcadaverine (MDC), SP600125, Z-VAD-fmk and 2′,7′-dichlorofluorescein diacetate (DCFH-DA) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
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