Sibecn1

Manufactured by GenePharma

Sourced in China, United States

The SiBECN1 is a laboratory equipment designed for nucleic acid amplification and detection. It serves as a versatile platform for various molecular biology applications, including gene expression analysis, pathogen detection, and diagnostic assays. The core function of the SiBECN1 is to facilitate the amplification and quantification of specific nucleic acid sequences in a controlled and reliable manner, enabling researchers and clinicians to obtain accurate and reproducible results.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Sistat3, by GenePharma (1 mentions) Dharmafect 4, by Thermo Fisher Scientific (1 mentions) Pcmv c flag vector, by Beyotime (1 mentions) Pgl3.0 enhancer, by Promega (1 mentions)

2 protocols using sibecn1

Silencing VEGFR2, STAT3, and BECN1

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SiVEGFR2, siSTAT3, siBECN1 and control siRNAs were obtained from GenePharma (Suzhou, Jiangsu, China). Osteosarcoma cells were transfected with siRNA by Lipofectamine 3000 (Invitrogen). The cells were cultivated for 48 h for further experiments.

Liu K., Ren T., Huang Y., Sun K., Bao X., Wang S., Zheng B, & Guo W. (2017). Apatinib promotes autophagy and apoptosis through VEGFR2/STAT3/BCL-2 signaling in osteosarcoma. Cell Death & Disease, 8(8), e3015-.

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Plasmid Constructs and RNA Interference

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pcDNA3.1-ASPP2 (full, (1–360), (360–925), (925–1128))-V5 was obtained from Dr Xin Lu's Lab at Ludwig Institute for Cancer Research, Oxford, UK. pcDNA3.0-flag-BECN1 was obtained from Prof. Mujun Zhao's Lab at Institute of Biochemistry and Cell Biology, Chinese Academy of Science, Shanghai, People's Republic of China. pCMV-p65/RelA-flag was constructed by PCR and inserted into pCMV-C-Flag vector (Beyotime Biotech. D2632, Shanghai, People's Republic of China). Various truncated BECN1 promoters were generated by PCR and inserted into reporter vector pGL3.0-enhancer (Promega, Madison, WI, USA). p40(phox)PX-EGFP, pFlag-BECN1 ((1–150), (150–241), (242–450)), pCI-neo-HA-hUVRAG and pCI-neo-HA-hATG14 were purchased from Addgene (Cambridge, MA, USA).
Small interfering RNAs targeting BECN1 (siBECN1) were generated by GenePharma (Shanghai, People's Republic of China) and were transfected with DharmaFECT4 (ThermoScientific, T-2004-02). Lentiviral plasmid vectors encoding short hairpin RNAs (shRNAs) targeting ASPP2 or scramble shRNA were generated and designated as LV-shASPP2 and LV-shNon, respectively. Further details are available in the Supplementary Material.

Chen R., Wang H., Liang B., Liu G., Tang M., Jia R., Fan X., Jing W., Zhou X., Wang H., Yang Y., Wei H., Li B, & Zhao J. (2016). Downregulation of ASPP2 improves hepatocellular carcinoma cells survival via promoting BECN1-dependent autophagy initiation. Cell Death & Disease, 7(12), e2512-.

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