Matrine (CAS: 519-02-8) was purchased from the National Institutes for Food and Drug Control, with a purity of 98.7%. Ribavirin (CAS: 36791-04-5) was purchased from Solarbio with a purity of 99%. Probe 1 was synthesized by Shanghai NF Biotechnology Co., Ltd. (Shanghai, China). Probe 2 was synthesized and presented by Associate Professor Chunli Wu from Zhengzhou University. Marc-145 cells were purchased from the China Institute of Veterinary Drugs Control (Beijing, China). The cell culture medium was DMEM medium containing 10% FBS, and the cell maintenance medium was DMEM medium containing 2% FBS. PRRSV was extracted from the diseased material and proliferated. The TCID50 in Marc-145 cells was 10−7.79/mL using the Reed–Muench method [44 (link)].
Ribavirin
Manufactured by Solarbio
Sourced in China
Ribavirin is a synthetic nucleoside analog used in the laboratory setting. It functions as an antiviral agent, inhibiting the replication of certain viruses.
Automatically generated - may contain errors
Lab products found in correlation
Antibody against md2 24182, by Abcam (2 mentions)
Mpo kit a044 1 1, by Nanjing Jiancheng (2 mentions)
Amiodarone, by Merck Group (1 mentions)
Delphinidin, by Merck Group (1 mentions)
Roxithromycin, by Merck Group (1 mentions)
Tamoxifen, by MedChemExpress (1 mentions)
Fluvastatin, by MedChemExpress (1 mentions)
Oseltamivir, by MedChemExpress (1 mentions)
Clemastine, by MedChemExpress (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Midecamycin, by MedChemExpress (1 mentions)
Acetylspiramycin, by MedChemExpress (1 mentions)
Amantadine, by Merck Group (1 mentions)
Chloroquine cq, by Sangon (1 mentions)
Clarithromycin, by MedChemExpress (1 mentions)
Fluoxetine, by MedChemExpress (1 mentions)
Bafilomycin a1, by Merck Group (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Caco 2, by American Type Culture Collection (1 mentions)
Cleaved caspase 3, by Abcam (1 mentions)
7 protocols using ribavirin
1
Matrine and Ribavirin Antiviral Assay
2
Antiviral and Anti-inflammatory Effects of Klebsiella pneumoniae Infection
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Klebsiella pneumoniae (BNCC-102997) was purchased from Beijing Beina Chuanglian Biotechnology Research Institute (Beijing, China). Influenza virus FM1 strain was provided by ABSL-2 laboratory, Institute of traditional Chinese medicine, Chinese Academy of Sciences (Beijing, China). Antibodies against MyD88 (#4283), TLR4 (#14358) were obtained from Cell Signaling (Beverly, MA, USA), and antibody against MD2 (#24182) was obtained from Abcam (Cambridge, MA, England). Ribavirin (20160308) was purchased from Solarbio (Beijing, China). Ceftriaxone sodium Injection was purchased from ReYoung Pharmaceutical Co., Ltd (Shandong, China). IL-1β, IL-6, and TNF-α ELISA kits were obtained from Neobioscience (Shenzhen, China). MPO kit (A044-1-1) was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Physiological saline for injection (L219012211) was purchased from Sichuan Kelun Pharmaceutical Co., Ltd. (Chengdu, China).
3
Plasmid Constructs and Viral Protein Production
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pCAGGS-RIG-I-Flag, pCAGGS-MDA5-Flag, pCAGGS-MOV10-Flag, pCAGGS-ZCCHC3-Flag, pCAGGS-DDX46-Flag, and pCAGGS-Serinc5-Flag, Porcine IFN-α (PoIFN-α) protein were prepared in our laboratory. Ribavirin was purchased from Beijing Solarbio Science and Technology Co., Ltd.
4
Matrine Inhibits PCV2 Infection in Mice
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Matrine (batch number: ZLSC2018032020) was purchased from Nanjing Zelang Meditech Ltd., China, and its purity was 98% by HPLC. The chemical structure of matrine was shown in Figure 7 . Ribavirin (Batch number: 36791-04-5) was purchased from Beijing Solarbio Technology Co., Ltd. (Beijing, China). The TCID50 of PCV2 was 10−5.4/0.1 mL. Female SPF KM mice were purchased from Beijing Vital River Laboratoty Animal Technology Co., Ltd. (Beijing, China, quality certificate number: 110011210115155452). All mice were raised in the Experimental Animal Management Center of Shanxi Agricultural University.
5
Antiviral Screening of Small Molecules
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Human cervix adenocarcinoma (Hela), adenocarcinomic human alveolar basal epithelial cells (A549), human embryonic kidney cells (HEK293T) and human colon adenocarcinoma cell (Caco2) were obtained from American Type Culture Collection and maintained in complete DMEM medium (containing 2 mmol/L l ‐Glutamine, 10% foetal bovine serum (Thermo Fisher Scientific), 100 μg/mL streptomycin and 100 U/mL penicillin) at 37°C with 5% CO2 incubation. The interferon receptor knockout (IFNAR1 KO) HEK293T cells and retinoic acid‐inducible gene‐I (RIG‐I) KO HEK 293T cells were kept in our laboratory. The influenza virus strains used in this study were A/WSN/33(H1N1) (WSN), which was kept in our laboratory. The IAV‐luc (PR8) strain was a gift from Prof. Ling Chen (Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences). AZ and erythromycin were obtained from BBI Life Sciences. Ribavirin was obtained from Solarbio. Oseltamivir, midecamycin, spiramycin, acetylspiramycin, clarithromycin, dirithromycin, tamoxifen, fluvastatin, fluoxetine and clemastine were obtained from MedChemExpress. Amiodarone, amantadine, roxithromycin, delphinidin and bafilomycin A1 were obtained from Sigma. Chloroquine (CQ) and amlodipine (Norvasc) were obtained from Sangon Biotech.
6
Antivirals Screening for Porcine Circovirus Type 2
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The non-PCV-infected pig kidney epithelial cell lines (PK-15) was cultured, passaged, and frozen in liquid nitrogen.
PCV2-SH strain was supplied by Professor Jiang Ping of Nanjing Agricultural University. PK-15 cells were used for virus propagation. 10 6.4 TCID 50 / mL of virus titer was determined by indirect immunofluorescence (IFA).
Matrine with 98.7% purity was purchased from the National Institutes for Food and Drug Control (lot number: 110805-201709). Osthole with 98.0% purity was purchased from Nanjing Zelang Biotechnology Co., Ltd (Lot number ZL20171212SCZS). Ribavirin with 99.0% purity was purchased from Beijing Solarbio Technology Co., Ltd (CAS 36791-04-5). The chemical structures of Matrine and Osthole were shown in Fig. 1.
Antibodies against Cap was respectively purchased from Biorbyt LLC. (California, Britain); GRP78 PERK eIF2 p-eIF2 ATF4 Chop Bcl-2 Bax and Cleaved-caspase 3 were purchased from Abcam (Cambridge, USA); Cleaved-caspase 9 and p-PERK were respectively were purchased from Bioworld Technology Co., Ltd. (Beijing, China) and immunology Biology Technology Co., Ltd. (Beijing, China); GAPDH and horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Wuhan Sanying Biology Technology Co., Ltd.(Wu Han,China)and ComWin Biotech Co., Ltd. (Beijing, China).
PCV2-SH strain was supplied by Professor Jiang Ping of Nanjing Agricultural University. PK-15 cells were used for virus propagation. 10 6.4 TCID 50 / mL of virus titer was determined by indirect immunofluorescence (IFA).
Matrine with 98.7% purity was purchased from the National Institutes for Food and Drug Control (lot number: 110805-201709). Osthole with 98.0% purity was purchased from Nanjing Zelang Biotechnology Co., Ltd (Lot number ZL20171212SCZS). Ribavirin with 99.0% purity was purchased from Beijing Solarbio Technology Co., Ltd (CAS 36791-04-5). The chemical structures of Matrine and Osthole were shown in Fig. 1.
Antibodies against Cap was respectively purchased from Biorbyt LLC. (California, Britain); GRP78 PERK eIF2 p-eIF2 ATF4 Chop Bcl-2 Bax and Cleaved-caspase 3 were purchased from Abcam (Cambridge, USA); Cleaved-caspase 9 and p-PERK were respectively were purchased from Bioworld Technology Co., Ltd. (Beijing, China) and immunology Biology Technology Co., Ltd. (Beijing, China); GAPDH and horseradish peroxidase (HRP)-conjugated secondary antibodies were purchased from Wuhan Sanying Biology Technology Co., Ltd.(Wu Han,China)and ComWin Biotech Co., Ltd. (Beijing, China).
7
Investigating Klebsiella pneumoniae and Influenza Virus Interactions
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Klebsiella pneumoniae (BNCC-102997) was purchased from Beijing Beina Chuanglian Biotechnology Research Institute (Beijing, China). In uenza virus FM1 Strain was provided by ABSL-2 laboratory, Institute of traditional Chinese medicine, Chinese Academy of Sciences (Beijing, China). Antibodies against MyD88 (#4283), TLR4 (#14358) were obtained from Cell Signaling (Beverly, MA, USA), and antibody against MD2 (#24182) was obtained from Abcam (Cambridge, MA, England). Ribavirin (20160308) was purchased from Solarbio (Beijing, China). Ceftriaxone sodium Injection was purchased from ReYoung Pharmaceutical Co.,Ltd (Shandong, China). IL-1β, IL-6, and TNF-α ELISA kits were obtained from Neobioscience (Shenzhen, China). MPO kit (A044-1-1) was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Physiological saline for injection (L219012211) was purchased from Sichuan Kelun Pharmaceutical Co., Ltd. (Chengdu, China).
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