Monkey liver S9 fraction was obtained from BD Bioscience Pharmingen (San Diego, CA). Trizma pre-set crystals, reduced β-nicotinamide adenine dinucleotide phosphate (NADPH), alamethicin, buspirone, potassium phosphate buffer, Krebs-Henseleit buffer powder, trypan blue, and uridine 5’-diphosphoglucuronic acid (UDPGA) were obtained from Sigma (St. Louis, MO). LC-MS grade acetonitrile (ACN), water, and methanol (MeOH) were purchased from EM Science (Gibbstown, N.J.). Deuterated acetonitrile and deuterated methanol were obtained from Cambridge Isotopes Laboratories Inc. (Andover, MA). NMR tubes were acquired from Kimble/Chase Life Science (Vineland, NJ). Human recombinant CYP (Supersomes) CYP3A4, CYP2C9, CYP2C19, CYP1A2, CYP2D6, CYP2C8, and CYP2E1 were purchased from BD Biosciences (San Jose, CA). Cryopreserved pooled mouse, rat, dog, monkey, and human hepatocytes together with hepatocyte thawing medium were obtained from Bioreclamation IVT (Baltimore, MD). The test article (diamine purine containing parent compound) was synthesized in Celgene.
β nicotinamide adenine dinucleotide phosphate reduced nadph
Manufactured by Merck Group
Sourced in United States
β-nicotinamide adenine dinucleotide phosphate reduced (NADPH) is a cofactor used in various enzymatic reactions. It serves as a reducing agent and is involved in cellular metabolic processes.
Automatically generated - may contain errors
Lab products found in correlation
Hydrogen peroxide h2o2, by Merck Group (2 mentions)
Potassium phosphate buffer, by Merck Group (1 mentions)
Buspirone, by Merck Group (1 mentions)
Trypan blue, by Merck Group (1 mentions)
Trizma preset crystals, by Merck Group (1 mentions)
Uridine 5 diphosphoglucuronic acid udpga, by Merck Group (1 mentions)
Alamethicin, by Merck Group (1 mentions)
Cyp1a2, by BD (1 mentions)
Cyp2c9, by BD (1 mentions)
Cyp2c19, by BD (1 mentions)
Cyp2d6, by BD (1 mentions)
Cyp2c8, by BD (1 mentions)
Cyp2e1, by BD (1 mentions)
Coenzyme a coa, by Merck Group (1 mentions)
P coumaric acid, by Merck Group (1 mentions)
Sinapic acid, by Merck Group (1 mentions)
Ferulic acid, by Merck Group (1 mentions)
Folin, by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Triton 100, by Merck Group (1 mentions)
5 protocols using β nicotinamide adenine dinucleotide phosphate reduced nadph
1
Characterization of CYP-Mediated Metabolism
2
Rice Plant Tissue Collection and Metabolite Extraction
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Sterilized seeds of wild-type rice plants (O. sativa L. spp. Japonica cv. Dongjin) were germinated on Murashige and Skoog (MS) medium (Duchefa, Harlem, Netherlands) in a growth chamber with a 12 h photoperiod and temperature of 28°C. Ten-day old seedlings were transferred to soil and grown in a greenhouse at 28°C during the day and 20°C at night. Stem and leaf samples were collected from 10-week-old rice plants, and panicle samples were collected from 14-week-old rice plants. Root and shoot samples were collected from 10-day old rice seedlings.
p-Coumaric acid, ferulic acid, sinapic acid, coenzyme-A (CoA) and reduced β-nicotinamide adenine dinucleotide phosphate (NADPH) for hydroxycinnamoyl-CoA production were purchased from Sigma-Aldrich (St. Louis, MO, USA). Reagents for buffers, media and other solutions were obtained from Sigma-Aldrich and Duchefa.
p-Coumaric acid, ferulic acid, sinapic acid, coenzyme-A (CoA) and reduced β-nicotinamide adenine dinucleotide phosphate (NADPH) for hydroxycinnamoyl-CoA production were purchased from Sigma-Aldrich (St. Louis, MO, USA). Reagents for buffers, media and other solutions were obtained from Sigma-Aldrich and Duchefa.
3
Biochemical Analysis of Oxidative Stress
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DATS was obtained from LKT laboratories (St. Paul, MN, USA). Folin, GSH, hydrogen peroxide (H2O2), dithiothreitol (DTT), phenylmethylsulfonyl fluoride (PMSF), paraformaldehyde (PFA), β-nicotinamide adenine dinucleotide phosphate reduced (NADPH), GR, pepstatin A, aprotinin, leupeptin, Nonidet® P40, Triton® × 100, ethylenediaminetetraacetic acid (EDTA), phosphatases cocktail inhibitor and bovine serum albumin (BSA) were obtained from Sigma (St. Louis, MO, USA). Sodium azide (NaN3) was obtained from Hycel de México (Zapopan, Jalisco, México). Hydroxyethyl piperazineethanesulfonic acid (HEPES) was from MP Biomedicals (Solon, OH, USA). Primary antibodies rabbit anti-GST (110-218; sc-33614) and rabbit anti-matrix metalloproteinase-9 (MMP-9; H-129; sc-10737) were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The primary antibody rabbit anti-malondialdehyde (MDA; ab6463) was obtained from Abcam (Cambridge, MA, USA). Primary antibodies rabbit anti-SOD1 (ADI-SOD-101-E) and rabbit anti-SOD2 (ADI-SOD-111) were purchased from Enzo Life Science (Farmingdale, NY, USA). TransAM Nrf2 ELISA kit was from Active Motif (Carlsbad, CA, USA) and Universal L Kit SAB-System HRP was from DAKO (Carpinteria, CA, USA). All other reagents were obtained from other known commercial local sources.
4
Antioxidant Properties of Turkish Honey
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Thiobarbituric acid (TBA), butylated hydroxytoluene (BHT), trichloroacetic acid (TCA), ethylenediaminetetraacetic acid (EDTA), reduced glutathione (GSH), metphosphoric acid, 5,5’dithiobis-(2-nitrobenzoic acid) (DTNB), trihydroxymethyl aminomethane (Tris), 1-chloro-2,4-dinitrobenzene (CDNB), oxidized glutathione (GSSG), β-Nicotinamide adenine dinucleotide phosphate (reduced) (NADPH), potassium dihydrogen phosphate (KH2PO4), hydrogen peroxide (H2O2) and sodium chloride (NaCl) of technical grade used in this study were supplied by Sigma Chemical Co. (St. Louis, MO, USA). Kits for antioxidant enzymes analysis were supplied by Randox Laboratories Ltd. The honey sample of East Anatolia region was purchased from a local honey store in Van, Turkey.
5
Antioxidant Enzyme Activity Assay
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Reagents were obtained as follows: Tris base, tert-butyl hydroperoxide (TBHP), coenzyme A (CoA) lithium salt, sucrose, β-nicotinamide adenine dinucleotide phosphate reduced (NADPH), glutathione reductase from baker’s yeast, D-panthenol (PL), sodium 2-oxoglutarate, sodium succinate, and sodium azide, from Sigma-Aldrich (Merck, Darmstadt, Germany); and 2-thiobarbituric acid, reduced and oxidized glutathione, 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB), trichloroacetic acid (TCA), and Folin–Ciocalteu’s reagent, from AppliChem GmbH (Darmstadt, Germany). All reagents were of analytical grade, HPLC grade, or the best available pharmaceutical grade. All solutions were prepared using water purified by the Milli-Q system.
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