Hla dr bv605 g46 6

Manufactured by BD

HLA-DR-BV605 (G46-6) is a fluorescent-labeled antibody used in flow cytometry applications. It binds to the HLA-DR antigen expressed on the surface of antigen-presenting cells.

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Lab products found in correlation

Fortessa facs analyzer, by BD (1 mentions) Cd3 apc cy7 sk7, by BD (1 mentions) Cd19 bv421 hib19, by BioLegend (1 mentions) Cd38 bv786 hit2, by BD (1 mentions) Cd25 pecy7 bc96, by BioLegend (1 mentions) Cd19 pecy5 hib19, by BioLegend (1 mentions) Pd 1 bv421 eh12.2h7, by BioLegend (1 mentions) Cd3 apc r700 ucht1, by BD (1 mentions) Cd69 pe fn50, by BD (1 mentions)

Spelling variants of this product

HLA-DR (247 citations) HLA-DR BV605 (6 citations) HLA-DR BV605 (clone G46-6, (3 citations)

2 protocols using hla dr bv605 g46 6

Multicolor Flow Cytometry for Immune Cell Analysis

Cited 1 time

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Flow cytometric assays were performed as described previously (11 (link), 12 (link)). Briefly, PBMCs were thawed and co-cultured in a 24-well plate at 1.0 × 10⁶ cells/well in RPMI 1,640 with 10% fetal calf serum for 24 h at 37°C and 5% CO₂. After overnight culture, the cells were labeled with the following anti-human monoclonal antibodies: CD3-APC Cy7 (SK7; BD Bioscience), CD4-Alexa700 (RPA-T4; BD Bioscience), CD8-BV480 (PRA-T8, BD Bioscience), CD19-BV421 (HIB19, Bio-Legend), HLA-DR-BV605 (G46-6, BD Bioscience), CD38-BV786 (HIT2, BD Bioscience), PD1-phycoerythrin (PE) (EH12.1, Bio-Legend), CD25-PECy7 (BC96; Bio-Legend), and IL7Ra (CD127)-BV650 (A019D5; Bio-Legend). Isotype controls, 7-AAD, and Fc blocker were included in all experiments. Multicolor flow cytometric analysis was performed using a BD FACS Fortessa analyzer (BD Biosciences) and FlowJo ver. 10 software (Tree Star Inc., Ashland, OR).

Takiguchi S., Tomita Y., Uehara S., Tateishi K., Yamamoto N, & Nakamura M. (2022). Immunological imprint on peripheral blood in kidney transplant recipients after two doses of SARS-CoV-2 mRNA vaccination in Japan. Frontiers in Medicine, 9, 999374.

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FACS Analysis of Immune Cell Markers

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The following monoclonal antibodies were used for FACS: CCR7 APC-Cy7 (G043H7, Biolegend, 353211), CD69 PE (FN50, BD, 555531), CD3 APC R700 (UCHT1, BD, 565119), PD-1 BV421 (EH12.2H7, Biolegend, 329920), HLA-DR BV605 (G46-6, BD, 562845), CXCR5 BB515 (RF8B2, BD, 564624), CD14 PE-Cy5 (61D3, abcam, ab25395), CD16 PE-Cy5 (3G8, Biolegend, 3012010), CD19 PE-Cy5 (HIB19, Biolegend, 302210), CD45RA PE CF594 (HI100, BD, 562298), CD8 PE-Cy5.5 (RPA-T8, eBioscience, 35-0088-42), and CD4 PE Cy7 (RPA-T4, Biolegend, 300512).

Wu V.H., Nobles C.L., Kuri-Cervantes L., McCormick K., Everett J.K., Nguyen S., del Rio Estrada P.M., González-Navarro M., Torres-Ruiz F., Ávila-Ríos S., Reyes-Terán G., Bushman F.D, & Betts M.R. (2020). Assessment of HIV-1 integration in tissues and subsets across infection stages. JCI Insight, 5(20), e139783.

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