Migration assays were conducted using Transwell plates with 8 μm pore size membranes (Corning Inc., USA). After incubation for 12 hr, cells remaining in the upper side of the filter were removed with cotton swabs. The cells attached on the lower surface were fixed and stained using crystal violet and washed with water. Cells were counted with five high power fields per membrane and results were presented as the mean number of cells migrated per field per membrane. All experiments were conducted in triplicate.
Transwell plates with 8 μm pore size membranes
Manufactured by Corning
Sourced in United States
Transwell plates with 8 μm pore size membranes are a type of cell culture insert system designed for various in vitro applications. The 8 μm pore size in the semi-permeable membrane allows for the study of cell migration, invasion, and permeability. These plates enable the separation of cells in an upper and lower chamber, facilitating the assessment of cellular interactions and barrier functions.
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Lab products found in correlation
3 protocols using transwell plates with 8 μm pore size membranes
1
Transwell Cell Migration Assay
2
Transwell Assay for Cell Migration and Invasion
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Migration and invasion assays were conducted using transwell plates with 8-μm pore size membranes (Corning Inc., USA) as described previously [16 (link)]. After incubation for 4 h (for migration assays) or 24 h (for invasion assays), cells remaining in the upper side of the filter were removed with cotton swabs. The cells attached on the lower surface were fixed and stained using crystal violet and washed with water. Cells were counted with five high power fields per membrane, and results were presented as the mean number of cells migrated per field per membrane. All experiments were conducted in triplicate.
3
Transwell Migration and Invasion Assay
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