Log In Sign up
The largest database of trusted experimental protocols

Ml sa1

Manufactured by Merck Group
Visit Supplier
Sourced in United States

The ML-SA1 is a laboratory equipment product manufactured by Merck Group. It is designed for analytical applications in scientific research and testing environments. The core function of the ML-SA1 is to perform precise measurements and analyses of samples.

Automatically generated - may contain errors

Lab products found in correlation

Ionomycin, by Merck Group (4 mentions) Bafilomycin a1, by Merck Group (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (4 mentions) Mk6 83, by Merck Group (3 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (3 mentions) Diaphoto tmd inverted microscope, by Nikon (2 mentions) A 769662, by Merck Group (2 mentions) Ml si3, by Merck Group (2 mentions) Il 15, by Miltenyi Biotec (2 mentions) Ym201636, by InvivoGen (2 mentions) Bictegravir, by LGC (2 mentions) Cuprizone bis cyclohexanone oxaldihydrazone, by Merck Group (2 mentions) Fluoromyelin green, by Thermo Fisher Scientific (2 mentions) Dq red bsa, by Thermo Fisher Scientific (2 mentions) Lysosensor yellow blue dnd 160, by Thermo Fisher Scientific (2 mentions) Leu leu methyl ester hydrobromide, by Merck Group (2 mentions) Bafilomycin a, by Merck Group (2 mentions) Pf 429242, by Bio-Techne (1 mentions) Praziquantel, by Merck Group (1 mentions) Serotonin, by Merck Group (1 mentions)

15 protocols using ml sa1

1

Optimizing Cell Signaling Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
UA (Sigma-Aldrich) was dissolved in DMSO and used at a final concentration of 1 µM. AnCoA4 was dissolved in DMSO and used at a final concentration of 50 µM. PF-429242 (Tocris) was dissolved in DMSO and used at a final concentration of 500 nM. TG (Sigma-Aldrich) was dissolved in DMSO and used at a final concentration of 500 nM. Ionomycin (Sigma-Aldrich) was dissolved in DMSO and used at a final concentration of 2.5 to 5 µM. ML-SA1 (Sigma-Aldrich) was dissolved in DMSO and used at a final concentration of 10 µM.
Tiscione S.A., Vivas O., Ginsburg K.S., Bers D.M., Ory D.S., Santana L.F., Dixon R.E, & Dickson E.J. (2019). Disease-associated mutations in Niemann-Pick type C1 alter ER calcium signaling and neuronal plasticity. The Journal of Cell Biology, 218(12), 4141-4156.
+ Open protocol
+ Expand
2

Measuring Lysosomal Calcium Release in Podocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 18–24 h after nucleofection with GCaMP3-ML1, podocytes were used for experiments [11 (link)]. The fluorescence intensity at 470 nm (F470) was recorded with a digital camera (Nikon Diaphoto TMD Inverted Microscope). Metafluor imaging and analysis software were used to acquire, digitize and store the images for offline processing and statistical analysis (Universal Imaging, Bedford Hills, NY, USA). Lysosomal Ca2+ release was measured under a ‘low’ external Ca2+ solution, which contained 145 mM NaCl, 5 mM KCl, 3 mM MgCl2, 10 mM glucose, 1 mM EGTA and 20 mM HEPES (pH 7.4). GPN (Cayman Chemical, Ann Arbor, MI, USA) was used as positive control to induce Ca2+ release from lysosomes in podocytes. ML-SA1 (Sigma-Aldrich Chemicals, St. Louis, MO, USA) was used as a potent TRPML channel agonist.
Li G., Huang D., Bhat O.M., Poklis J.L., Zhang A., Zou Y., Kidd J., Gehr T.W, & Li P.L. (2020). Abnormal podocyte TRPML1 channel activity and exosome release in mice with podocyte-specific Asah1 gene deletion. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1866(2), 158856.
+ Open protocol
+ Expand
3

Fluorescent Probes for Cellular Imaging

Check if the same lab product or an alternative is used in the 5 most similar protocols
ML-SA1, MK6–83, praziquantel, and serotonin were from Sigma-Aldrich (St. Louis MO). LysoTracker RED DND-99 was from Life Technologies, Inc. ML-SI3 was obtained from Enamine, code (s) EN 300–314172. Stock solutions were made in dimethyl sulfoxide (DMSO, ATCC, Manassas, VA) and subsequently diluted to an appropriate concentration in culture or recording media. All oligonucleotides were bought from Integrated DNA Technologies (IDT, Coralville, IA).
Bais S., Norwillo A., Ruthel G., Herbert D.R., Freedman B.D, & Greenberg R.M. (2022). Schistosome TRPML channels play a role in neuromuscular activity and tegumental integrity. Biochimie, 194, 108-117.
+ Open protocol
+ Expand
4

Culturing HeLa Cells and Primary Cortical Neurons

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were maintained in DMEM (Dulbecco’s modified Eagle’s medium; Lonza) supplemented with 10% FBS (Atlanta Biologicals) at 37ºC in the presence of 5% CO2. tBHP was from Invitrogen (Carlsbag, CA) and ML-SA1 was from Sigma-Aldrich (St. Louis, MO). Primary cortical neuron cultures were prepared as previously described [44 (link)], from E15–16 C57BL/6 (Charles River) mouse embryos. The neurons were plated on poly-L-lysine-coated four-well chambered slides (Nunc Laboratory-Tek; Fisher Scientific, Agawam, MA, USA) at 3105 cells/cm2 in serum-free Neurobasal media (Gibco, Thermo Fisher) and supplemented with 0.75mM L-glutamine (BioWhittaker, Walkersville, MD, USA) and B27 supplement (Gibco).
Ravi S., Peña K.A., Chu C.T, & Kiselyov K. (2016). Biphasic regulation of lysosomal exocytosis by oxidative stress. Cell calcium, 60(5), 356-362.
+ Open protocol
+ Expand
5

Measuring Lysosomal Calcium Release in Podocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols
At 18–24 h after nucleofection with GCaMP3-ML1, podocytes were used for experiments [11 (link)]. The fluorescence intensity at 470 nm (F470) was recorded with a digital camera (Nikon Diaphoto TMD Inverted Microscope). Metafluor imaging and analysis software were used to acquire, digitize and store the images for offline processing and statistical analysis (Universal Imaging, Bedford Hills, NY, USA). Lysosomal Ca2+ release was measured under a ‘low’ external Ca2+ solution, which contained 145 mM NaCl, 5 mM KCl, 3 mM MgCl2, 10 mM glucose, 1 mM EGTA and 20 mM HEPES (pH 7.4). GPN (Cayman Chemical, Ann Arbor, MI, USA) was used as positive control to induce Ca2+ release from lysosomes in podocytes. ML-SA1 (Sigma-Aldrich Chemicals, St. Louis, MO, USA) was used as a potent TRPML channel agonist.
Li G., Huang D., Bhat O.M., Poklis J.L., Zhang A., Zou Y., Kidd J., Gehr T.W, & Li P.L. (2020). Abnormal podocyte TRPML1 channel activity and exosome release in mice with podocyte-specific Asah1 gene deletion. Biochimica et biophysica acta. Molecular and cell biology of lipids, 1866(2), 158856.
+ Open protocol
+ Expand
6

Characterization of TRPML1 Modulators

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following compounds were used: the TRPML1 inhibitor, EDME (10–50 µM, synthesis as previously described; Rühl et al., 2021 (link)); TRPML1 activators, ML-SA1 (10–50 µM, SML0627, Sigma-Aldrich), MLSA5 (10–50 µM, kind gift from Casma Therapuetics, Cambridge, MA, USA) and MK683 (synthesized as described previously, Chen et al., 2014 (link)); and concanamycin A (ConA, 1 µM, Santa Cruz Biotechnology, Dallas, Texas, USA).
Siow W.X., Kabiri Y., Tang R., Chao Y.K., Plesch E., Eberhagen C., Flenkenthaler F., Fröhlich T., Bracher F., Grimm C., Biel M., Zischka H., Vollmar A.M, & Bartel K. (2022). Lysosomal TRPML1 regulates mitochondrial function in hepatocellular carcinoma cells. Journal of Cell Science, 135(6), jcs259455.
+ Open protocol
+ Expand
7

Isolation and Treatment of Mouse CASMCs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolation of mouse CASMCs was described previously [34 (link)]. Briefly, CASMCs were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco Gaithersburg, MD, USA), supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin–streptomycin (Gibco Gaithersburg, MD, USA) in humidified 100% air and 5% CO2 mixture at 37 °C. Confluent cells (70%–80%) were treated with or without high phosphate (Pi) (3 mmol/L) [35 (link)] and then incubated with TRMPL1 channel agonist, MLSA-1 (10 μM, Sigma-Aldrich Chemicals, St. Louis, MO, USA) and TRMPL1 channel blocker, verapamil (10 μM, Cayman Chemical, Ann Arbor, MI, USA) incubated for 24 h [31 (link),55 (link)].
Bhat O.M., Yuan X., Camus S., Salloum F.N, & Li P.L. (2020). Abnormal Lysosomal Positioning and Small Extracellular Vesicle Secretion in Arterial Stiffening and Calcification of Mice Lacking Mucolipin 1 Gene. International Journal of Molecular Sciences, 21(5), 1713.
+ Open protocol
+ Expand
8

TRPML1 Agonist and Antagonist Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The TRPML1 agonist MK6-83 (Sigma-Aldrich) was dissolved in DMSO and used at 20 μΜ unless specified otherwise. The TRPML1 antagonist ML-SI3 (enamine) was dissolved in DMSO and used at 50 μΜ. The chemical modulators ML-SA1, A-769662, bafilomycin A1, ionomycin, MGR1, and MGR2 were used as indicated in the figure legends, and all were obtained from Sigma-Aldrich. Clinical-grade recombinant human IL-2 (Proleukin) was obtained from Clinigen via Sykehusapoteket (no. 600373). IL-15 was used at 1 or 10 ng/ml (Miltenyi Biotec).
Clement D., Szabo E.K., Krokeide S.Z., Wiiger M.T., Vincenti M., Palacios D., Chang Y.T., Grimm C., Patel S., Stenmark H., Brech A., Majhi R.K, & Malmberg K.J. (2023). The Lysosomal Calcium Channel TRPML1 Maintains Mitochondrial Fitness in NK Cells through Interorganelle Cross-Talk. The Journal of Immunology Author Choice, 211(9), 1348-1358.
+ Open protocol
+ Expand
9

Lysosomal and Calcium Signaling in Autophagy

Check if the same lab product or an alternative is used in the 5 most similar protocols
In this study, we used the following chemicals from Sigma-Aldrich (St Louis, MO): PP242 (P0037), EBSS (Earle’s Balanced Salt Solution; E2888), thapsigargin (T9033), bafilomycin A1 (B1793), A23187 (C7522), ionomycin (I0634), glycyl-L-phenylalanine2-naphthylamide (GPN, G9512), ML-SA1 (SML0627), and carbonyl cyanide 3-chlorophenylhydrazone (CCCP, C2759). LysoTracker (L7528), Fura-2 acetoxymethyl ester (Fura-2 AM; F1221), BAPTA-AM (B1205), and ProLong™ Gold Antifade Mountant with DAPI (P10144) were obtained from Life Technologies. Torin1 (4247) was purchased from Tocris (Bristol, UK). Antibodies against p-RPS6 KB1/S6K (9234), RPS6KB/S6K (9202), p-RPS6/S6 (2211, 2215), p-ULK1 (6888), LAMP1 (9091), CANX (calnexin) (2679), TFEB (4240), PPP3 CA/calcineurin A (2614), SQSTM1/p62 (39749), and histone (9717) were obtained from Cell Signaling Technology. Antibodies against ACTB/actin (sc-47778), GAPDH (sc-47724), TUBA/tubulin (sc-5286), ITPR1 (sc-271197), ITPR2 (sc-398434), and ITPR3 (sc-7277) were obtained from Santa Cruz Biotechnology. Antibodies against MCOLN1/TRPML1 (ab28508) and RAB7A (ab50533) were obtained from Abcam. Antibody against HA (11867423001) was from Roche Diagnostics. Antibody against TFE3 (HPA023881) was purchased from Sigma-Aldrich. ZFYVE27 (LS-C660718) was from LS-Bio. Antibody against LC3B (NB600-1384) was from Novus.
Kim H.K., Lee G.H., Bhattarai K.R., Lee M.S., Back S.H., Kim H.R, & Chae H.J. (2020). TMBIM6 (transmembrane BAX inhibitor motif containing 6) enhances autophagy through regulation of lysosomal calcium. Autophagy, 17(3), 761-778.
+ Open protocol
+ Expand
10

TRPML1 Agonist and Antagonist Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
The TRPML1 agonist MK6-83 (Sigma-Aldrich) was dissolved in DMSO and used at 20 μΜ unless specified otherwise. The TRPML1 antagonist ML-SI3 (enamine) was dissolved in DMSO and used at 50 μΜ. The chemical modulators ML-SA1, A-769662, bafilomycin A1, ionomycin, MGR1, and MGR2 were used as indicated in the figure legends, and all were obtained from Sigma-Aldrich. Clinical-grade recombinant human IL-2 (Proleukin) was obtained from Clinigen via Sykehusapoteket (no. 600373). IL-15 was used at 1 or 10 ng/ml (Miltenyi Biotec).
Clement D., Szabo E.K., Krokeide S.Z., Wiiger M.T., Vincenti M., Palacios D., Chang Y.T., Grimm C., Patel S., Stenmark H., Brech A., Majhi R.K, & Malmberg K.J. (2023). The Lysosomal Calcium Channel TRPML1 Maintains Mitochondrial Fitness in NK Cells through Interorganelle Cross-Talk. The Journal of Immunology Author Choice, 211(9), 1348-1358.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!