For TEM analysis, cells were grown on Transwell filters and fixed with 4% paraformaldehyde and 2% glutaraldehyde for 90 min at room temperature. Cell samples were then processed for embedding in Epoxy, TAAB 812 Resin (TAAB Laboratories, Berkshire, UK) following standard procedures. Orthogonal and parallel (from the bottom to the top of the cell) 80-nm-thick ultrathin sections were stained with saturated uranyl acetate and lead citrate by standard procedures. Samples were examined at 80 kV in a Jeol JEM-1010 (Tokyo, Japan) electron microscope. Pictures were taken with a TemCam-F416 (4 K × 4 K) digital camera (TVIPS, Gauting, Germany).
Temcam f416 4 k 4 k digital camera
Manufactured by TVIPS
Sourced in Germany
The TemCam-F416 is a 4K × 4K digital camera designed for transmission electron microscopes (TEMs). It features a large sensor size and high resolution for capturing detailed images of samples at the nanoscale.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using temcam f416 4 k 4 k digital camera
1
TEM Analysis of Cell Ultrastructure
2
Transmission Electron Microscopy of Cells
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For transmission electron microscopy analysis, cells grown on Transwell filters were fixed with 4% paraformaldehyde and 2% glutaraldehyde for 90 min at room temperature. Cell samples were then processed for embedding in Epoxy, TAAB 812 Resin (TAAB Laboratories, Berkshire, UK) according to standard procedures. Orthogonal and parallel (from the bottom to the top of the cell) 80-nm-thick ultrathin sections were stained with saturated uranyl acetate and lead citrate by standard procedures. Samples were examined at 80 kV in a Jeol JEM-1010 (Tokyo, Japan) electron microscope. Pictures were taken with a TemCam-F416 (4 K×4 K) digital camera (TVIPS, Gauting, Germany).
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