Multiplex elisa based assay

Manufactured by Mesoscale

The Multiplex ELISA-based assay is a laboratory equipment product that allows for the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to measure the concentration of various target molecules, such as proteins, peptides, or hormones, in a sample.

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Lab products found in correlation

Quantikine elisa kit, by R&D Systems (3 mentions)

Spelling variants of this product

Multiplex ELISA (11 citations) Multiplex assay (8 citations) Multiplex ELISA assay (2 citations)

3 protocols using multiplex elisa based assay

Quantifying Intestinal Biomarkers via ELISA

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Serum levels of intestinal-fatty acid binding protein (I-FABP), sCD14, and sCD163 were quantified using ELISA (Quantikine ELISA kit, R&D systems) while sICAM-1 and sVCAM-1 were measured using multiplex ELISA-based assay (Meso-Scale Discovery). Assays were performed in the same UW laboratory in duplicate. The inter-assay coefficients of variation were <11%.

TEMU T.M., ZIFODYA J.S., POLYAK S.J., WAGONER J., WANJALLA C.N., MASYUKO S., NYABIAGE J., KINUTHIA J., BLOOMFIELD G.S., PAGE S.T, & FARQUHAR C. (2021). Antiretroviral Therapy Reduces but Does Not Normalize Immune and Vascular Inflammatory Markers in Adults with Chronic HIV Infection in Kenya.. AIDS (London, England), 35(1), 45-51.

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Multiplex Analysis of Plasma Immune Markers

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Plasma levels of IL-2, IL-4, IL-5, IL-6, IL-2p70, IL-13, IL-15, IL-17A, IFN-γ, and TNF-α were measured using the multiplex ELISA-based assay (Meso-Scale Discovery). Samples with a coefficient of variation greater than 30% were re-ran. Plasma biomarkers of intestinal barrier dysfunction (intestinal-fatty acid binding protein [I-FABP]) and monocyte/macrophage activation markers (soluble CD14) were quantified using ELISA (Quantikine ELISA kit, R&D systems). Assays were performed in duplicate and in accordance with manufacturers’ protocols.

Li Z., Zhang Y., Ku L., Wilkinson K.D., Warren S.T, & Feng Y. (2001). The fragile X mental retardation protein inhibits translation via interacting with mRNA. Nucleic Acids Research, 29(11), 2276-2283.

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Plasma Cytokine and Intestinal Barrier Profiling

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Plasma levels of IL-2, IL-4, IL-5, IL-6, IL-2p70, IL-13, IL-15, IL-17A, IFN-γ, and TNF-α were measured using the multiplex ELISA-based assay (Meso-Scale Discovery). Samples with a coefficient of variation >30% were re-ran. Plasma biomarkers of intestinal barrier dysfunction [intestinal-fatty acid binding protein (I-FABP)] and monocyte/macrophage activation markers (soluble CD14) were quantified using ELISA (Quantikine ELISA kit, R&D Systems). Assays were performed in duplicate and in accordance with manufacturers’ protocols.

Temu T.M., Polyak S.J., Wanjalla C.N., Mandela N.A., Dabee S., Mogaka J.N., Masyuko S., Longernecker C., Shakil S., Chohan B., Page S.T., Lacourse S.M., Gitura B., Crothers K., Oyugi J., Jaspan H., Farquhar C, & Zifodya J.S. (2023). Latent tuberculosis is associated with heightened levels of pro-and anti-inflammatory cytokines among Kenyan men and women living with HIV on long-term antiretroviral therapy. AIDS (London, England), 37(7), 1065-1075.

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