Accu chek active blood glucose meter kit

Forty-eight animals, 3 months old and with an average body weight of 230 ± 14 g, were recruited together. The control groups (CT1–CT4) were injected with 0.1-M sodium citrate buffer, pH 4.5, as a vehicle²¹ (link)^,22 (link) and were sacrificed after 1, 2, 3, and 4 months, respectively. DM was induced in 24 rats by injecting STZ (35 mg/kg) intraperitoneally.²¹ (link)^,22 (link) DM1 and DM2 rats were sacrificed 1 and 2 months, respectively, after STZ injection, and CT1 and CT2 served as the controls. DRC 1 and DRC2 rats were sacrificed 3 and 4 months, respectively, after STZ injection, and CT3 and CT4 served as the controls. Glucose levels were quantitated using a drop of blood from the tail vein using an ACCU-CHEK Active Blood Glucose Meter Kit (Roche Diagnostics, Basel, Switzerland).

The antidiabetic activity study was carried out after the test animals were acclimatized for 5 days. Animals were divided into three groups, each group consisting of three animals. The groups of animal are shown in Table 1.
Before induction, the blood glucose levels of each animal in three groups were determined. Induction of diabetes was done by intravenous injection for 3 times of alloxan solution in aqua pro injection (150 mg/kg body weight) into the rabbit after fasting for 10 h. After that, rabbits with blood glucose levels of >200 mg/dL were included in the study. Treatment with FDT-As-NE and pure astaxanthin were started 48 h after alloxan injection. Blood collection was carried out through the rabbit ear vein and blood glucose levels were determined before and 2 h after treatment everyday by using Accu-Chek^® active blood glucose meter kit (Roche, Germany).