Male C57BL/6J (wild-type: WT) were studied at seven weeks of age (Japan SLC, Inc., Hamamatsu, Japan). The mice were housed individually in plastic cages at 24 °C ± 1 °C with lights on from 07:00 to 19:00. The mice were provided with either AIN-93M-based control-diet (CD) and diets with 60% of calories from fat containing lard (Lard), beef tallow (Beef), hydrogenated coconut oil (Coconut), linseed oil (Linseed), corn oil (Corn), olive oil (Olive), soybean oil (Soybean), and cocoa butter (Cocoa) (Oriental Yeast Japan, Table S1 and Figure S1 ) and water ad libitum for three months. To examine effect of HFDs in addition to the carcinogen, 1-methyl-3-nitro-1-nitrosoguanidine (MNNG; Tokyo Chemical Industry Co., Ltd., Tokyo, Japan), 100 mg/L MNNG in distilled water was provided to mice twice a week from five weeks of age. At seven weeks of age, in addition to MNNG administration, mice were fed CD or HFDs with Lard, Linseed, Olive, or Cocoa for three months. Animal care and experiments were conducted in accordance with the guidelines of the Prefectural University of Hiroshima Animal Care and Use Committee and Review Board (18A-008).
1 methyl 3 nitro 1 nitrosoguanidine mnng
Manufactured by Tokyo Chemical Industry
Sourced in Japan
1-methyl-3-nitro-1-nitrosoguanidine (MNNG) is a chemical compound commonly used in research laboratories. It functions as an alkylating agent, a type of chemical that can modify DNA and other biological molecules.
Automatically generated - may contain errors
Lab products found in correlation
C57bl 6j, by Japan SLC (1 mentions)
7 12 dimethylbenz a anthracene dmba, by Tokyo Chemical Industry (1 mentions)
3 methylcholanthrene 3 mc, by Merck Group (1 mentions)
Glycidol, by Fujifilm (1 mentions)
Glyoxal, by Fujifilm (1 mentions)
6 thioguanine 6 tg, by Merck Group (1 mentions)
7 12 dimethylbenz a anthracene dmba, by Merck Group (1 mentions)
4 protocols using 1 methyl 3 nitro 1 nitrosoguanidine mnng
1
High-fat Diet Effects on MNNG-induced Carcinogenesis
2
Herbal Formula for Liver Protection
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1-Methyl-3-nitro-1-nitrosoguanidine (MNNG) was purchased from Tokyo Chemical Industry, Japan (Lot. NH8JH-BM). Vitacoenzyme tablets were purchased from LEPUHENGJIUYUAN Co., Ltd., China (No. 20190803). Sodium salicylate was purchased from Sangon Biotech, China (Lot. EC11BA0022). The following formula granules were provided by Guangdong Yifang Pharmaceutical Co., Ltd.: Artemisia capillaris Thunb. (Yinchen), 15 g; Scutellaria baicalensis Georgi (Huangqin), 12 g; Coptis chinensis Franch.(Huanglian), 12 g; Scutellaria barbata D. Don (Banzhilian), 15 g; Scleromitrion diffusum (Willd.) R. J. Wang (Baihuasheshecao), 15 g; Isatis tinctoria L.(Banlangen), 15 g; Pogostemon cablin (Blanco) Benth.(Guanghuoxiang), 9 g; Lobelia chinensis Lour. (Banbianlian), 15 g; Sophora flavescens Aiton (Kushen), 10 g; Eupatorium fortunei Turcz.(Peilan), 9 g; and Gynostemma pentaphyllum (Thunb.) Makino (Jiaogulan), 15 g.
3
Genotoxic Compound Acquisition for Research
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1-methyl-3-nitro-1-nitrosoguanidine (MNNG; CASRN. 70-25-7), methyl methanesulfonate (MMS; CASRN. 66-27-3), 4-nitroquinoline N-oxide (4NQO; CASRN. 56-57-5), propylene oxide (PO; CASRN. 75-56-9), 2-acetamidofluorene (2AAF; CASRN. 53-96-3) and 7,12-dimethylbenz[a]anthracene (DMBA; CASRN. 57-97-6) were purchased from Tokyo Chemical Industry Co. Ltd. (Tokyo, Japan). Glyoxal (CASRN. 107-22-2) and glycidol (CASRN. 556-52-5) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Formaldehyde (FA; CASRN. 50-00-0), 2-aminoanthracene (2AA; CASRN. 613-13-8) and 3-methylcholanthrene (3MC; CASRN. 56-49-5) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
4
Evaluating Fortilin's Impact on CHO Cell Mutagenesis
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CHO cells were seeded at 1x105 cells/well in a 48-well plate and incubated for 36–40 h. The cells were exposed to a concentration of either 0.5 or 1 μg/ml of rFm-Fortilin (FL) protein for 24, 48, or 72 h at 37°C, after which the medium containing the rFm-Fortilin (FL) protein was removed. 1-methyl-3-nitro-1-nitrosoguanidine (MNNG) (Tokyo Chemical Industry Co., Ltd, Japan) and 7,12-dimethylbenz (a) anthracene (DMBA) (Sigma Aldrich, US) mutagens were used as positive controls, while PBS and DMSO were used as negative controls. The cells were cultured in DMEM for 8 days. Mutant cells were identified by treatment with 5 μg/ml 6-thioguanine (6-TG) (Sigma Aldrich, US) in DMEM. After 10 days of growth, the cells were stained with Trypan blue and counted to determine the mutant frequency.
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