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Ab126352

Manufactured by Abcam
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Ab126352 is a lab equipment product offered by Abcam. It is a device used for the detection and quantification of specific molecules or analytes in a sample. The core function of this product is to provide a reliable and accurate measurement of the target substance.

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Lab products found in correlation

Cyclin a, by Santa Cruz Biotechnology (1 mentions) α actinin, by Proteintech (1 mentions) Trypsin with edta, by Thermo Fisher Scientific (1 mentions) Proliferating cell nuclear antigen (pcna), by Santa Cruz Biotechnology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dmem high glucose medium, by Thermo Fisher Scientific (1 mentions) β tubulin, by Signalway Antibody (1 mentions) Penicillin streptomycin, by BasalMedia (1 mentions) Ab49405, by Abcam (1 mentions) Ab27591, by Abcam (1 mentions) Alexa fluor 594 conjugated antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 conjugated antibody, by Thermo Fisher Scientific (1 mentions) Sc 9081, by Santa Cruz Biotechnology (1 mentions)

2 protocols using ab126352

1

Comprehensive Antibody and Cell Culture Protocols

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The antibody against DPY30 used for IHC was from Abcam (ab126352). The primary antibodies used for WB and IHC included: PCNA (Santa Cruz, sc-25280), Ki67 (Santa Cruz, sc-23900), cyclin A (Santa Cruz, sc-271682). And other antibodies applied to WB as follows: α-actinin (Proteintech, 11313-2-AP), β-tubulin (Signalway Antibody, #48885), H3K4me3 (CST, C42D8, #9751), Histone H3 (CST, D1H2, #4499), cyclin B1(sc-245), cyclin D1 (sc-8396), cyclin E (sc-377100), p21 (sc-6246). Anti-mouse or rabbit secondary antibodies were purchased from Sigma. FBS, high glucose DMEM medium, and Trypsin (with EDTA) were purchased from Gibco (Thermo Fisher Scientific, USA). penicillin-streptomycin purchased from BasalMedia. Other reagents were all analytical grade. Other relevant kits or special supplies will be described below.
Su W.C., Mao X.M., Li S.Y., Luo C.Y., Fan R., Jiang H.F., Zhang L.J., Wang Y.T., Su G.Q, & Shen D.Y. (2023). DPY30 Promotes Proliferation and Cell Cycle Progression of Colorectal Cancer Cells via Mediating H3K4 Trimethylation. International Journal of Medical Sciences, 20(7), 901-917.
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2

Immunofluorescence Antibody Protocol

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Mouse monoclonal anti-DDX4 antibody used for immunofluorescence was purchased from Abcam (ab27591); Rabbit polyclonal anti-DPY30 antibody used for immunofluorescence was purchased from Abcam (ab126352); Rabbit polyclonal was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
anti-POU5F1 antibody used for immunofluorescence was purchased from Santa Cruz (sc-9081); Rabbit polyclonal anti-STRA8 antibody used for immunofluorescence was purchased from Abcam (ab49405); Alexa Fluor 488-conjugated antibody and Alexa
Fluor 594-conjugated antibody were purchased from Life Technologies.
Zhao Z.H., Ma J.Y., Meng T.G., Wang Z.B., Yue W., Zhou Q., Li S., Feng X., Hou Y., Schatten H., Ou X.H, & Sun Q.Y. (2020). Single-cell RNA sequencing reveals the landscape of early female germ cell development. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(9).
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