We imaged and analyzed four isogenic cell lines, termed SW28 (link),29 (link), HS, TE, and WM. The SW cells are a pair of isogenic cancer cell lines: SW480 (ATCC CCL-228)—colorectal adenocarcinoma cells, and SW620 (ATCC CCL-227)—a metastatic form of these cancer cells collected from a lymph node of the same patient. The HS cells are a pair of a normal skin cell line, HS895.Sk (ATCC CRL-7636) and a melanoma cancer cell line, HS895.T (ATCC CRL-7637), taken from the same patient. The TE cells are a pair of a normal skin cell line, TE353.Sk (ATCC CRL-7761), and a melanoma cancer cell line, TE354.T (ATCC CRL-7762), are both taken from the same patient. The WM cells are a pair of a melanoma skin cell line, WM115 (ATCC CRL-1675), and a metastatic melanoma skin cell line, WM266.4 (ATCC CRL-1676), both taken from the same patient.
All the cells were grown in Dulbecco's modified Eagle medium (ATCC, SN. 30-2002), supplemented with 10% fetal bovine serum (BI, SN. 04-007-1A). 2 mM L-glutamine (BI, SN. 03-020-1B) were added for the WM cell lines. The cells were incubated under standard humidity and temperature of 37 °C with 5% CO2 until 80% confluence was reached. Before imaging, the cells were trypsinized for suspension and supplemented with a suitable medium.
All the cells were grown in Dulbecco's modified Eagle medium (ATCC, SN. 30-2002), supplemented with 10% fetal bovine serum (BI, SN. 04-007-1A). 2 mM L-glutamine (BI, SN. 03-020-1B) were added for the WM cell lines. The cells were incubated under standard humidity and temperature of 37 °C with 5% CO2 until 80% confluence was reached. Before imaging, the cells were trypsinized for suspension and supplemented with a suitable medium.