Cell counting kit 8 c0038

Manufactured by Beyotime

Sourced in China

The Cell Counting Kit-8 (C0038) is a colorimetric assay for the determination of cell viability and cytotoxicity. It utilizes a highly water-soluble tetrazolium salt, WST-8, which is reduced by dehydrogenases in living cells to generate a yellow-colored formazan dye. The amount of formazan dye produced is directly proportional to the number of living cells.

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Cell Counting Kit (21 citations) C0038 (13 citations)

2 protocols using cell counting kit 8 c0038

Elucidating Mesangial Cell Pathways

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A human mesangial cell line (HMC cell line, FH0241) was obtained from FuHeng Biology Co. (Shanghai, China). DHT (116064-77-8, purity ≥ 98%) was acquired from Krre Technology Co. (Beijing, China). Mannitol (69-65-8) was obtained from Macklin Biochemical Co. (Shanghai, China). The following antibodies: GAPDH (1:1000, ab8245), α-smooth muscle actin (1:1000, ab7817), Collagen I (1:1000, ab260043), Fibronectin (1:1000, ab2413), PI3KCA (1:1000, ab40776), PI3K (1:1000, ab191606), p-PI3K (1:1000, ab182651), AKT (1:1000, ab179463), and p-AKT (1:1000, ab192623) were bought from Abcam Co. (Cambridge, United Kingdom). Goat Anti-Rabbit IgG H&L (HRP, A0208), Rabbit Anti-Mouse IgG H&L (HRP, A0216), and Rabbit Anti-Mouse IgG H&L (Alexa Fluor 488, A0428) were purchased from Beyotime (Shanghai, China). DAPI Staining Solution (C1006) and Cell Counting Kit-8 (C0038) were also obtained from Beyotime (Shanghai, China). Glucose, Trypsin, Dulbecco’s Modified Eagle’s Medium (DMEM), and fetal bovine serum (FBS) were purchased from GIBCO. The Mouse Albumin ELISA Kit (ab207620) was purchased from Abcam.

Wang Y., Liu Y., Chen S., Li F., Wu Y., Xie X., Zhang N., Zeng C., Bai L., Dai M., Zhang L, & Wang X. (2023). The protective mechanism of Dehydromiltirone in diabetic kidney disease is revealed through network pharmacology and experimental validation. Frontiers in Pharmacology, 14, 1201296.

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Baicalin Inhibits Pulmonary Hypertension

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Baicalin were obtained from Nanjing Jingzhu Biotechnology Co. Ltd. (Nanjing, China). Monocrotalin (MCT, C2401) was purchased from Sigma‒Aldrich (St. Louis, MO, USA). Transforming growth factor-beta 1 (TGF-β1, HY-P70543) and calpain Inhibitor MDL-28170 (HY-18236) were purchased from MedChemExpress. Antibodies against calpain-1 (A1172), Phosphoinositide 3-kinase (PI3K, A0265), Protein kinase B (AKT, A11016), Phosphorylated protein kinase B (p-AKT, AP0140) were purchased from ABclonal (Wuhan, China). Antibodies against Platelet endothelial cell adhesion molecule-1 (CD31, 66065-2-Ig), Smooth muscle actin α (α-SMA, 14395-1-AP), Vimentin (10366-1-AP), VE-cadherin (66804-1-Ig) and fluorescein (FITC)-conjugated Affinipure goat anti-mouse lgG (H + L) (SA00003-1) were purchased from Proteintech (Wuhan, China). DyLight 594 AffiniPure goat Anti-rabbit IgG (H + L) (E032420-01) was purchased from EarthOx. The EdU Cell Proliferation Kit (C0071S) and Cell Counting Kit-8 (C0038) were obtained from Beyotime Biotechnology (Shanghai, China).

Jiang H.X., Wang X.D., Wang H.X, & Liu T. (2023). Baicalin attenuates pulmonary vascular remodeling by inhibiting calpain-1 mediated endothelial-to-mesenchymal transition. Heliyon, 9(12), e23076.

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