Cy3-labeled probes specific to circFAM114A2 and FAM-labeled probes specific to miR-222-3p/miR-146a-5p were constructed and produced through GenePharma (Shanghai, China). Probe signals were identified through a Fluorescent In Situ Hybridization Kit (GenePharma, Shanghai, China) as per established protocol. Pictures were attained on a Zeiss LSM880 NLO confocal microscope system.
Lsm880 nlo confocal microscope system
Manufactured by Zeiss
Sourced in Germany
The LSM880 NLO confocal microscope system is a high-performance confocal laser scanning microscope designed for advanced imaging applications. The system features a multi-photon excitation capability, allowing for deep tissue imaging with reduced phototoxicity. The core function of the LSM880 NLO is to provide high-resolution, three-dimensional imaging of biological samples.
Automatically generated - may contain errors
3 protocols using lsm880 nlo confocal microscope system
1
Circulating RNA Detection by FISH
2
High-Resolution Imaging of Cardiac and Embryonic Samples
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The following systems were used. For confocal imaging, Zeiss LSM 880-NLO confocal microscope system (Jena, Germany) with an Airyscan detector with a FAST module on a Zeiss Axio observer Z1 inverted microscope equipped with an internal spectral QUASAR detector. Stereo images of the heart or embryos were harvested by a stereoscope (Leica M205 FA, Wetzlar, Germany).
3
Quantitative Confocal Imaging of Organoids
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Samples were imaged using confocal laser scanning microscopy (Nikon Instruments A1 Confocal Laser Microscope; Zeiss LSM 880 NLO Confocal Microscope System). Images were analyzed using Fiji (https://imagej.net/Fiji ). For cell quantification in the organoids, DAPI positive cells were counted and used for normalization against the target cell marker of interest across at least three z-planes throughout each organoid for each target cell marker.
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