Apc anti human cd81

Manufactured by BioLegend

APC Anti-Human CD81 is a fluorescently-labeled antibody that binds to the CD81 cell surface protein, which is expressed on a variety of human cell types. This product can be used for the identification and analysis of CD81-expressing cells in flow cytometry applications.

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Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Mouse anti gapdh, by Proteintech (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Minimum essential medium (mem), by Corning (1 mentions) 4 6 diamidino 2 phenylindole dihydrochloride, by Thermo Fisher Scientific (1 mentions) Pe anti human cd63, by BioLegend (1 mentions) Mg132, by Selleck Chemicals (1 mentions) Ab108252, by Abcam (1 mentions)

Spelling variants of this product

Anti-CD81 (9 citations) Anti-CD81-APC (4 citations) APC-conjugated anti-human CD81 antibody (2 citations)

2 protocols using apc anti human cd81

Exosome Surface Marker Identification

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For directly exosome surface staining, EVs were incubated with preconjugated FITC mouse anti-human CD63 (BioLegend) and APC Anti-Human CD81 (BioLegend) for 30 min at room temperature. After incubation, the samples were washed with PBS, ultracentrifuged and then resuspended in PBS buffer. Stained samples were analyzed using ZE5 (Bio-Rad) flow cytometer and FlowJo software (Treestar).

Lo T.W., Zhu Z., Purcell E., Watza D., Wang J., Kang Y.T., Jolly S., Nagrath D, & Nagrath S. (2020). Microfluidic Device for High-Throughput Affinity-based Isolation of Extracellular Vesicles. Lab on a chip, 20(10), 1762-1770.

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Antibodies for EV and Protein Detection

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D MEM, FBS, and penicillin-streptomycin were obtained from Gibco. MEM was purchased from Corning. Lipofectamine 2000 and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) were obtained from Thermo Fisher Scientific. MG132 was purchased from Selleck. All the pools of siRNA were obtained from the RiboBio Company (Guangzhou, China). The antibodies used for flow cytometry included PE-conjugated antibodies against CD63 (PE-anti-human CD63) (353003, BioLegend), APC-anti-human CD9 (312107, BioLegend), and APC-anti-human CD81 (349509, BioLegend). The antibodies used for Western blot included rabbit-anti-ubiquitin (10201-2-AP, Proteintech), mouse-anti-RFP-Tag (T0055, Affinity), rabbit-anti-ACE2 (ab108252, Abcam), mouse-anti-calnexin (66903-1-IG, Proteintech), rabbit-anti-CD63 (25682-1-AP, Proteintech), rabbit-anti-HA (51064-2-AP, Proteintech), rabbit-anti-GFP-Tag (50430-2-AP, Proteintech), mouse-anti-GAPDH (60004-1-Ig, Proteintech), and rabbit-anti-PSMD12 (39119, Signalway Antibody). The secondary antibodies used for developing targeted proteins in Western blotting assays included IRDye 680RD Goat anti-Mouse (926-68070) and IRDye 800CW Goat anti-Rabbit (926-32211).

Lv X., Chen R., Liang T., Peng H., Fang Q., Xiao S., Liu S., Hu M., Yu F., Cao L., Zhang Y., Pan T., Xi Z., Ding Y., Feng L., Zeng T., Huang W., Zhang H, & Ma X. (2024). NSP6 inhibits the production of ACE2-containing exosomes to promote SARS-CoV-2 infectivity. mBio, 15(3), e03358-23.

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