Opn ab8448

Manufactured by Abcam

ab8448 is an antibody that recognizes the OPN protein. OPN is a secreted glycosylated phosphoprotein involved in bone mineralization and remodeling.

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Lab products found in correlation

Western blocking buffer, by Beyotime (1 mentions) Metformin hydrochloride, by MedChemExpress (1 mentions) Quinidine, by MedChemExpress (1 mentions) Phorbol 12 myristate 13 acetate, by MedChemExpress (1 mentions) Ab34710, by Abcam (1 mentions) Ml385, by MedChemExpress (1 mentions) Ripa buffer, by Beyotime (1 mentions) β actin, by Proteintech (1 mentions) Bca kit, by Beyotime (1 mentions) Ab5694, by Abcam (1 mentions) H2dcfda, by Beyotime (1 mentions) Sm22α ab14106, by Abcam (1 mentions) Goat anti mouse igg, by Proteintech (1 mentions) β actin antibody, by Proteintech (1 mentions)

2 protocols using opn ab8448

TGF-β2 Cytokine Modulation of Cellular Pathways

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The TGF-β2 cytokine was purchased from Pepro Tech. Glyceraldehyde-3-phosphatedehydrogenase (GAPDH), horseradish peroxidase (HRP)-conjugated secondary antibodies, RIPA buffer, H2DCF-DA, BCA kit, western blocking buffer, H₂DCF-DA were the products of Beyotime (China). The nucleoside 5-aminoimidazole-4-carboxamide riboside (AICAR), metformin hydrochloride, Phorbol 12-myristate 13-acetate (PMA), ML385 (an Nrf2 inhibitor) (CAS No.: 846557-71-9), Atropine (CAS No.: 51-55-8) and Quinidine (CAS No.: 56-54-2) were acquired from MedChemExpress (MCE) (China). Antibodies against AKT, p-AKT, AMPK and p-AMPK (172) were purchased from CST. Antibodies against superoxide dismutase 1 (SOD1), SOD2, γ-glutamyl cysteine synthetase (γ-GCS), Catalase (CAT), smad2/3 and β-actin were purchased from Proteintech. Antibodies against Nrf2, α-smooth muscle actin (α-SMA) (ab5694), Osteopontin (OPN) (ab8448), collagen-I, p-smad2/3 and fibronectin (ab34710) were purchased from Abcam. Antibody against p-Nrf2(S40) was provided by Santa Cruz Biotechnology. The cell culture reagents were the products of Gibco Laboratories (CAS No.:16000-044).

Li X., Leng Y., Jiang Q., Wang Z., Luo P., Zhang C., Chen L., Wang Y., Wang H., Yue X., Shen C., Zhou Y., Shi C, & Xie L. (2020). Eye Drops of Metformin Prevents Fibrosis After Glaucoma Filtration Surgery in Rats via Activating AMPK/Nrf2 Signaling Pathway. Frontiers in Pharmacology, 11, 1038.

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Investigating the Role of Daxx in VSMC Proliferation

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NC was synthesized by the pharmacology laboratory of Hunan University of Chinese Medicine (purity = 98%). Valsartan(B2214) was purchased from Apexbio biotechnology. Angiotensin II (AngII, A9525-1 mg) and MTT(M-2128) was purchased from Sigma-Aldrich. BRDU Cell Proliferation Assay(2750) was purchased from Millipore biotechnology. Daxx Antibody (sc-7152) was purchased from Santa Cruz biotechnology. PTEN Rabbit mAb(9188S), Phospho-Akt (Ser473) Rabbit mAb(4060S), Akt Rabbit mAb(4691S), and α-SMA(D4K9N) were purchased from Cell Signaling technology. OPN(ab8448) and SM22α(ab14106) were purchased from abcam technology. β-actin antibody(010–858), Goat-anti-rabbit IgG(H + L)(SA00001-2) and Goat anti Mouse IgG(H + L)(SA00001-1) were purchased from proteintech biotechnology. Lamin B antibody(15,082,820) was purchased from Wannlei biotechnology. HSP90 antibody(126–1026) were purchased from Shanghai Dianyin biotechnology. Nuclear Protein Extraction Kit(R0050) was purchased from Solarbio life sciences. BeyoClick™ EdU Cell Proliferation Kit with Alexa Fluor 647(C0081S) was purchased from Beyotime biotechnology. pHIV-EGFP-HA-Daxx lentiviral liquid was purchased from Beijing Kainuo Saisi biotechnology. VSMCs was purchased from central south university.

Sun S.Y., Cao Y.M., Huo Y.J., Qiu F., Quan W.J., He C.P., Chen Y., Liao D.F, & Tuo Q.H. (2021). Nicotinate-curcumin inhibits AngII-induced vascular smooth muscle cell phenotype switching by upregulating Daxx expression. Cell Adhesion & Migration, 15(1), 116-125.

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