The largest database of trusted experimental protocols

6 protocols using methanol

1

Chitosan-Diatomite Composite Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals were of analytical
reagent grade. Monopotassium phosphate (KH2PO4), sodium hydroxide (NaOH), sodium carbonate (Na2CO3), potassium nitrate (KNO3), potassium chloride
(KCl), chitosan (90% of deacetylation degree), zinc nitrate hexahydrate,
2-methylimidazole, and methanol were purchased from Tianjin Kermel
chemical reagent Co., Ltd. (Tianjin, China). Diatomite (D) was purchased
from Tianjin Damao Chemical Reagent Co., Ltd. (Tianjin, China).
+ Open protocol
+ Expand
2

Synthesis of Fructose Derivatives

Check if the same lab product or an alternative is used in the 5 most similar protocols
Methanol, dimethylformamide, dichloromethane, acetone, ethyl acetate, trifluoroacetic acid, and petroleum ether were purchased from Tianjin Kermel Chemical Reagent Co., Ltd (China). The above reagents were of analytical grade. Deionized water (filtered through a 0.22 μm membrane filter, >18.2 MΩ cm) was purified using a Milli-Q system from Millipore. Silica gel was purchased from Qingdao Haiyang Chemical Reagent Co., Ltd (China). Phenylalanine ethyl ester, di-O-isopropylidene-2,3:4,5-β-d-fructose and di-O-isopropylidene-2,3:4,5-β-d-fructopyranosyl trifluoromethanesulfonate were synthesized according to ref. 18 and 19 , respectively.
+ Open protocol
+ Expand
3

Azadirachtin Extraction and Derivatization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Methanol of HPLC grade was obtained from Tianjin Kermel Chemical Reagent Co., Ltd. (Tianjin, China). Chloroform and acetone were of analytical grade and obtained from the National Pharmaceutical Group (Shanghai, China). The ultrapure water was prepared by ELGA LabWater system. Pyridine, methoxylamine hydrochloride, and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA, 98%) with trimethylchlorosilane (TMCS, 1%) were obtained from Aladdin (Shanghai, China). Azadirachtin (>90%) was kindly provided by associate Professor Yong-Qing Tian of the Key Laboratory of Natural Pesticide and Chemical Biology of the Ministry of Education of South China Agricultural University.
+ Open protocol
+ Expand
4

Isovanillin-Based Organic Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isovanillin was supplied by Shandong Moon Fairy Biological Technology Co., Ltd. Reference substance 3‐hydroxy‐4‐methoxy benzal acrolein was synthesized by us. Methanol of HPLC grade was acquired from Tianjin Kermel Chemical Reagent Co., Ltd. Acetaldehyde, hydrochloric acid, and NaOH of analytical grade were purchased from Guangzhou Chemical Reagent Co., Ltd. All others chemicals were of analytical grade.
+ Open protocol
+ Expand
5

Analytical Method for Folic Acid

Check if the same lab product or an alternative is used in the 5 most similar protocols
FF was obtained from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China); 10% FF injection was obtained from Guangdong Dahuanong Animal Health Products Co., Ltd. (Guangdong, China); HP-β-CD was obtained from Shandong Binzhou Zhiyuan Biotechnology Co., Ltd. (Shandong, China); and chromatographic-grade methanol and acetonitrile were purchased from Tianjin Kermel Chemical Reagent Co. Ltd (Tianjin, China). All the other chemicals and reagents were of analytical grade and used as received.
+ Open protocol
+ Expand
6

Synthesis and Characterization of PVP-Coated Silver Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols
Polyvinylpyrrolidone (PVP K30) and silver nitrate (AgNO3) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Sodium hydrate (NaOH), sodium carbonate (Na2CO3), sodium chloride (NaCl), triethanolamine (TEA), ammonia solution (NH3·H2O), CTAB, and methanol were provided by Tianjin Kermel Chemical Reagent Co., Ltd. (Tianjin, China). Absolute ethanol (EtOH) was obtained from Anhui Ante Food Co., Ltd. (Suzhou, China). LA, TEOS, DA, TMB, F127, PEI, 1-tetradecanol (TD), N-hydroxysuccinimide (NHS), sodium sulfate (Na2S·9H2O), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), and MTT were obtained from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China). DOX was purchased from Shanghai Macklin Biochemical Co., Ltd. Fetal bovine serum (FBS) was provided by Hangzhou Sijiqing Bioengineering Material Co., Ltd. (Hangzhou, China). Dulbecco’s modified eagle medium (DMEM), Rhodamine 123 (Rh 123), trypsin-EDTA, phosphate-buffered saline (PBS), and dimethyl sulfoxide (DMSO) were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). The HepG2 cell line was provided by Procell Life Science Technology Co., Ltd. (Wuhan, China). All reagents were directly used without any further purification.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!