Plasmid (pET28a-MSP1D1ΔH5) was purchased from Addgene (USA), used for transformation of E. coli BL21(DE3) strain. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was obtained from Tokyo Chemical Industry, Japan. Bio-beads SM-2 was obtained from BIORAD, USA. HiTrapTM Desalting Column was purchased from GE Healthcare Japan Ltd. Ferric ammonium citrate (FAC) was obtained from Wako Pure Chemical Industries, Ltd, Japan. 2-Nitroso-5- [N-n-propyl-N-(3-sulfopropyl) amino] phenol (Nitroso-PSAP) and DDM were obtained from Dojin Chemical Research Laboratory, Japan.
1 2 dimyristoyl sn glycero 3 phosphocholine
Manufactured by Tokyo Chemical Industry
Sourced in Japan
1,2-dimyristoyl-sn-glycero-3-phosphocholine is a synthetic phospholipid compound. It is a major component of biological cell membranes and can be used in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ferric ammonium citrate, by Fujifilm (2 mentions)
Bio beads sm 2, by Bio-Rad (2 mentions)
Hitrap desalting column, by GE Healthcare (1 mentions)
Chromatographic columns, by GE Healthcare (1 mentions)
Lc ms grade methanol, by Fujifilm (1 mentions)
Milli q system, by Merck Group (1 mentions)
High performance liquid chromatography grade chloroform, by Fujifilm (1 mentions)
Acetonitrile, by Fujifilm (1 mentions)
2 propanol, by Fujifilm (1 mentions)
Etodolac, by Fujifilm (1 mentions)
1 m ammonium formate solution, by Fujifilm (1 mentions)
N lauroyl d erythro sphingosylphosphorylcholine, by Avanti Polar Lipids (1 mentions)
Mitotracker red, by Thermo Fisher Scientific (1 mentions)
Trypsin edta 0.05, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by HiMedia (1 mentions)
Dansyl chloride, by Tokyo Chemical Industry (1 mentions)
2 1h benzotriazole 1 yl 1 1 3 3 tetramethyluronium hexafluorophosphate, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
4 protocols using 1 2 dimyristoyl sn glycero 3 phosphocholine
1
Recombinant Membrane Protein Purification
2
Recombinant MSP1D1ΔH5 Protein Production
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Plasmid (pET28a-MSP1D1ΔH5) used for the transformation of E. coli BL21(DE3) strain was purchased from Addgene (Watertown, MA, USA). Ferric ammonium citrate (FAC) was obtained from Wako Pure Chemical Industries, Ltd., Osaka, Japan. 2-Nitroso-5-[N-n-propyl-N-(3sulfopropyl) amino] phenol (Nitroso-PSAP) and Dodecyl-β-maltoside (DDM) were purchased from Dojindo Laboratories, Kumamoto, Japan. 1,2-Dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was from Tokyo Chemical Industry, Tokyo, Japan. Bio-beads SM-2 was obtained from BIORAD, Hercules, CA, USA. Chromatographic columns were purchased from GE Healthcare Japan Ltd., Tokyo, Japan. All other chemicals were obtained in the highest grade.
3
Lipid Extraction and Quantification
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LC-MS-grade methanol, 2-propanol, acetonitrile, high-performance liquid chromatography-grade chloroform, and 1 M ammonium formate solution were obtained from Wako (Osaka, Japan). Three reagents were used as internal standards (ISs): N-lauroyl-d -erythro-sphingosylphosphorylcholine was from Avanti Polar Lipids (Alabaster, AL, USA), etodolac was from Wako, and 1,2-dimyristoyl-sn-glycero-3-phosphocholine was from Tokyo Chemical Industry (Tokyo, Japan). Water was purified on a Milli-Q system (Merck Millipore, Bedford, MA, USA).
4
Synthesis and Characterization of PCDA Conjugates
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All the chemicals and solvents are commercially available and were used as received. 10,12-Pentacosadiynoic acid (PCDA), 2′,7′-dichlorofluorescin diacetate (CM-H2DCFDA), dimethyl sulfoxide (DMSO), (4-carboxybutyl)triphenylphosphonium bromide, and 2-(1h-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate were purchased from Sigma Aldrich (St. Louis, MO, USA. Dansyl chloride and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was purchased from Tokyo Chemical Industry Co. Ltd. Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), N-hydroxysuccinimide (NHS), triethylamine (TEA), N,N-diisopropylethylamine (DIPEA) and phosphate buffer saline (PBS) were purchased from Sisco Research Laboratories (SRL) Pvt. Ltd, India. Dulbecco's Modified Eagle Medium (DMEM) and Trypsin EDTA 0.05% were procured from Thermo Fisher Scientific (Waltham, MA, USA). MitoTracker Red was obtained from Invitrogen (Carlsbad, CA, USA). Fetal bovine serum (FBS) was purchased from Himedia (Mumbai, India). Other standard laboratory chemicals and reagents were purchased from Sisco Research Laboratories (SRL) Pvt. Ltd, India.
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