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Pe conjugated p y694 stat5 47 stat5

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PE-conjugated p-(Y694)STAT5 (47/STAT5) is a fluorescently-labeled antibody that recognizes the phosphorylated form of the STAT5 transcription factor at tyrosine 694. This product is designed for flow cytometry and other immunoassay applications that require the detection of activated STAT5.

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Lab products found in correlation

Rhil 7, by Thermo Fisher Scientific (2 mentions) Rpa t4, by Thermo Fisher Scientific (2 mentions) Bv711 clone a019d5, by BioLegend (2 mentions) Fitc conjugated cd127 ebiordr5, by Thermo Fisher Scientific (2 mentions) Bv421 conjugated, by BioLegend (2 mentions) Pe cy7 conjugated foxp3 pch101, by Thermo Fisher Scientific (2 mentions) V450 conjugated active caspase3 c92 605, by BD (2 mentions) Pe conjugated p y705 stat3 4 p stat3, by BD (2 mentions) Pe conjugated ctla 4 14d3, by Thermo Fisher Scientific (2 mentions) Rhil 6, by BD (2 mentions) Rhil 15, by Thermo Fisher Scientific (2 mentions) Interleukin 6 (il 6), by Thermo Fisher Scientific (2 mentions) Cycloheximide (chx), by Merck Group (2 mentions) Recombinant human il rhil 2, by Thermo Fisher Scientific (1 mentions)

2 protocols using pe conjugated p y694 stat5 47 stat5

1

Flow Cytometry Analysis of Immune Markers

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The following directly conjugated Abs were used in flow cytometry: BV711 or FITC-conjugated CD4 [OKT4 (Biolegend) or RPA-T4 (eBioscience)], BV711- (clone A019D5; Biolegend) or FITC-conjugated CD127 (eBioRDR5; eBioscience), PE- (BD Biosciences) or BV421-conjugated (Biolegend) CD25 (MA251), allophycocyanin-conjugated FOXP3 (236A/E7; eBioscience) or PE-Cy7–conjugated FOXP3 (PCH101; eBioscience), V450-conjugated active CASPASE3 (C92-605; BD Biosciences), PE-conjugated p-(Y705)STAT3 (4/P-STAT3; BD Biosciences), PE-conjugated p-(Y694)STAT5 (47/STAT5; BD Biosciences), PE-conjugated CTLA-4 (14D3; eBioscience). Recombinant human IL (rhIL)-2 (Roche), IL-6, rhIL-7, rhIL-15 (Peprotech), and rhIL-6 (BD Biosciences) were used at the concentrations indicated in the figure legends. Tocilizumab (TOC; anti–IL-6R) and infliximab (anti–TNF-α) were used at a final concentration of 10 μg/ml. Cycloheximide (Sigma) was used at a concentration of 50 μg/ml.
Bending D., Giannakopoulou E., Lom H, & Wedderburn L.R. (2015). Synovial Regulatory T Cells Occupy a Discrete TCR Niche in Human Arthritis and Require Local Signals To Stabilize FOXP3 Protein Expression. The Journal of Immunology Author Choice, 195(12), 5616-5624.
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2

Flow Cytometry-Based Immune Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following directly conjugated antibodies were used in flow cytometry: BV711 or FITC-conjugated CD4 (OKT4 (Biolegend) or RPA-T4 (eBioscience)), BV711- (clone A019D5, Biolegend) or FITC-conjugated CD127 (eBioRDR5, eBioscience), PE- (BD Biosciences) or BV421-conjugated (Biolegend) CD25 (MA251), APC-conjugated FOXP3 (236A/E7, eBioscience) or PE-Cy7-conjugated FOXP3 (PCH101, eBioscience), V450-conjugated active CASPASE3 (C92-605, BD Biosciences), PE-conjugated p-(Y705)STAT3 (4/P-STAT3, BD Biosciences), PE-conjugated p-(Y694)STAT5 (47/STAT5, BD Biosciences), PE-conjugated CTLA-4 (14D3, eBioscience). Recombinant human (rh) IL-2 (Roche), IL-6, rhIL-7, rhIL-15 (Peprotech) and rhIL-6 (BD Biosciences) were used at the concentrations indicated in the figure legends. Tocilizumab (anti-IL-6R) and Infliximab (anti-TNFα) were used at a final concentration of 10μg/ml. Cycloheximide (Sigma) was used at a concentration of 50μg/ml.
Bending D., Giannakopoulou E., Lom H, & Wedderburn L.R. (2015). Synovial Regulatory T Cells Occupy a Discrete TCR Niche in Human Arthritis and Require Local Signals To Stabilize FOXP3 Protein Expression. The Journal of Immunology Author Choice, 195(12), 5616-5624.
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