Peptides were synthesized with a free amine NH2 terminus and a free acid COOH terminus with standard Fmoc chemistry (Genaxxon Bioscience, Ulm, Germany). Anti-CD8 (RPA-T8, 1:100; SK1, 1:300), anti-CD38 (Hb7, 1:200), anti-CD45RA (HI100, 1:50), anti-IFN-γ (4S.B3, 1:50), and anti–programmed cell death protein 1 (PD-1) (EH12.1, 1:33) were purchased from BD Biosciences (Germany). Anti-Bcl-2 (Bcl-2/100, 1:200), anti-CCR7 (G043H7, 1:33), Anti-CD8 (RPA-T8, 1:400), and anti-CD127 (A019D5, 1:33) were purchased from BioLegend (San Diego, CA). Anti-CD14 (61D3, 1:100), anti-CD19 (HIB19, 1:100), anti-Eomes (WD1928, 1:50), anti-killer cell lectin-like receptor G1 (KLRG1) (13F12F2, 1:33), and anti-T-bet (4B10, 1:200) were purchased from Thermo Scientific. Anti-T-cell factor (1TCF1) (C63D9, 1:100) was purchased from Cell Signaling Technology (Danvers, MA). Tetrameric complexes of HLA-B*15:01/L-HDAg170–179 SMQGVPESPF were generated as described previously.8 (link)
Anti ccr7 g043h7
Manufactured by BioLegend
Sourced in Canada
Anti-CCR7 (G043H7) is an antibody product manufactured by BioLegend. It is designed for detection and analysis of the CCR7 receptor, which is involved in lymphocyte trafficking and migration.
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Lab products found in correlation
2 protocols using anti ccr7 g043h7
1
Peptide Synthesis and Immune Marker Analysis
2
Multiparametric Flow Cytometry Analysis
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Cells for flow cytometry analysis were harvested and stained with fluorescently labeled antibodies for 30 min at 4℃ in dark. Following antibodies were used: anti-CD80 (L307.4; BD Biosciences, San Diego, CA), anti-CD32 (FLI8.26; BD Biosciences), anti CD83 (HB15e; Biolegend, San Diego, CA), anti-CD137L (5F4; Biolegend), anti-CD3 (OKT3, Biolegend), anti-CD8 (RPA-T8, Biolegend), anti-CD45RO (UCHL1, Biolegend), anti-CCR7 (G043H7, Biolegend), and anti-CD62L (DREG-26, BD Bioscience). pp65 Tetramer (Proimmune, Oxford, MC, UK) was used for tetramer staining and cells were analyzed with Canto (BD Biosciences). For analysis, lymphocyte population was gated based on FSC and SSC and further gating of CD8+ T cells were done by gating CD3 and CD8 double positive cells. Gating of memory differentiation was done either with CD45RO and CD62L or with CD45RO and CCR7.
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