Rabbit anti gapdh antibody

Manufactured by Wuhan Servicebio Technology

Sourced in China

The Rabbit anti-GAPDH antibody is a primary antibody that recognizes the GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) protein. GAPDH is a key enzyme involved in the glycolytic pathway and is commonly used as a control or reference protein in various biochemical and cell biology experiments.

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GAPDH (104 citations) Anti-GAPDH (32 citations) GAPDH antibody (8 citations) Rabbit anti-GAPDH (7 citations) Anti-GAPDH antibody (5 citations)

4 protocols using rabbit anti gapdh antibody

Western Blotting Analysis of Liver Protein Expression

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Total protein was extracted in lysis buffer supplemented with protease and phosphatase inhibitors (Solarbio, Wuhan, China). Western blotting was carried out according to our previous publication (22 (link)), six zebrafish liver samples in each group were performed for Western bloting. The antibodies used were as follows: rabbit anti-GAPDH antibody (1:2000; servicebio), rabbit anti-COL1A1 antibody (1:1000; Wanleibio), rabbit anti-ACTA2 antibody (1:1000; Proteintech), rabbit anti-IL-1β antibody (1:2000; Wanleibio), mouse anti-IL10 antibody (1:1500; Proteintech), rabbit anti-DRP1 antibody (1:1500; Proteintech), rabbit anti-OPA1 antibody (1:1500; Proteintech), rabbit anti-MFN2 antibody (1:1500; Proteintech), rabbit anti-P-AMPK antibody (1:2000; Cell Signaling Technology), rabbit anti-AMPK antibody (1:1500; Proteintech), rabbit anti-PGC1α antibody (1:1000; Bioss), rabbit anti-NRF1 antibody (1:1000; Proteintech), rabbit anti-NRF2 antibody (1:2000; Proteintech), rabbit anti-TFAM antibody (1:2000; Proteintech), rabbit anti-PINK1antibody (1:2000; Proteintech), rabbit anti-PARKIN antibody (1:2000; Bioss) and rabbit anti-P62 antibody (1:2000; Proteintech). Protein expression level was normalized to that of GAPDH.

Zou Y.Y., Tang X.B., Chen Z.L., Liu B., Zheng L., Song M.Y., Xiao Q., Zhou Z.Q., Peng X.Y, & Tang C.F. (2023). Exercise intervention improves mitochondrial quality in non-alcoholic fatty liver disease zebrafish. Frontiers in Endocrinology, 14, 1162485.

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Antioxidant and Anti-inflammatory Effects of XYW

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The XYW (Tai Ji, China, batch number 1707029) and fluoxetine hydrochloride (FLX) (Patheon, France, 7686 A) were dissolved in pure water solution preparation (SOD, batch number 20180,309), malondialdehyde (MDA, batch number 20180,313) (GSH, batch number 20180,621), and GSH/glutathione disulfide (GSH/GSSG, batch number 20180,726) levels were assessed using commercially available kits (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Nitric oxide (NO) assay kit (Biyuntian, 0,72117,171,110, China). Rat interleukin 6 (IL6) ELISA Kit (MULTI SCIENCES, A30680231), rat interleukin 1β (IL1B) ELISA Kit (ExCell Bio, 21H183), rabbit anti-NTRK2 antibody (Cell Signaling Technology; Cat. No. #4603), rabbit anti-AKT1 antibody (Cell Signaling Technology; Cat. No. #4691S), rabbit anti-p-AKT1 antibody (Cell Signaling Technology; Cat. No. #4060S), rabbit anti-HMOX1 antibody (Cell Signaling Technology; Cat. No. #S2206S), rabbit anti-PIK3CA antibody (Servicebio; Cat. No. #LS190932), rabbit anti-GAPDH antibody (Servicebio; Cat. No. #GB11002), rabbit anti-SOD1 antibody (Novus Biologicals; Cat. No. #03253462C2B), rabbit anti-NFE2L2 antibody (Invitrogen; Cat. No. #TK2668681A), rabbit anti-brain-derived neurotrophic factor (BDNF) antibody (Abcam; Cat. No. #ab108319) and anti-rabbit IgG HRP-linked antibody (Servicebio; Cat. No. #GB23303).

Ji Y., Luo J., Zeng J., Fang Y., Liu R., Luan F, & Zeng N. (2021). Xiaoyao Pills Ameliorate Depression-like Behaviors and Oxidative Stress Induced by Olfactory Bulbectomy in Rats via the Activation of the PIK3CA-AKT1-NFE2L2/BDNF Signaling Pathway. Frontiers in Pharmacology, 12, 643456.

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Molecular Mechanisms of Antidepressant Effects

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Xiaoyao Pills (XYW) (TaiJi, China), fluoxetine hydrochloride (FLX) (Patheon, France) and Amitriptyline hydrochloride (Sigma, USA) were dissolved in 0.9% saline to prepare solutions. Paeoniflorin (C₂₃H₂₈O₁₁), Liquiritin (C₂₁H₂₂O₉), Glycyrrhizic acid (C₄₂H₆₂O₁₆), Ligustilide (C₁₂H₁₄O₂) (Cheng Du Ai Fa, China). LPS (Escherichia coli 055:B5) was provided by Sigma (St. Louis, MO). Rabbit anti-TrkB antibody (1:1000; Cell Signaling Technology; Cat. No. #4603), rabbit anti-cAMP response element-binding protein (CREB) antibody (1:1000; Cell Signaling Technology; Cat. No. #9197S), rabbit anti-p-CREB antibody (1:1000; Cell Signaling Technology; Cat. No. #9198S), rabbit anti-β-Tubulin antibody (1:1000; Cell Signaling Technology; Cat. No. #2128). Rabbit anti-brain-derived neurotrophic factor (BDNF) antibody (1:1000; Abcam; Cat. No. #ab108319). Rabbit anti-DLG4, PSD95-specific, polyclonal antibody (1:1000; Proteintech; Cat. No. #20665-1-AP) and rabbit anti-synaptophysin polyclonal antibody (1:1000; Proteintech; Cat. No. #17785-1-AP). Rabbit anti-GAPDH antibody (1:1000; Servicebio; Cat. No. #GB11002). Rabbit anti-IL-6 polyclonal antibody (1:300; Servicebio; Cat. No. #GB11117) and Alexa Fluor 488 goat anti-rabbit IgG (1:400; Servicebio; Cat. No. #GB25303).

Shi B., Luo J., Fang Y., Liu X., Rao Z., Liu R, & Zeng N. (2019). Xiaoyao Pills Prevent Lipopolysaccharide-Induced Depression by Inhibiting Inflammation and Protecting Nerves. Frontiers in Pharmacology, 10, 1324.

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Plasma Membrane Protein Isolation and Fractionation

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Minute^TM Plasma Membrane Protein Isolation and Cell Fractionation Kit (Invent, Plymouth, USA) were adopted to separate the total and plasma membrane KCNB1 proteins. We collected the total protein fractions before cellular component separation and isolated plasma membrane proteins according to the manufacturer's protocol. The proteins of CHO-K1 co-expressing variants and wild-type were extracted using ice-cold RIPA buffer with a ratio of 100:1 protease inhibitor cocktails (Beyotime, Shanghai, China). The total amount of plasmid DNA here maintained 4 ug.
20 ug of samples of proteins were separated by 10% sodium dodecyl sulfate– polyacrylamide (SDS-PAGE), transferred to PVDF membranes, blocked in protein free rapid blocking buffer (Epizyme, Shanghai, China), and incubated with mouse anti-Kv2.1 (1:1000; Abcam, ab192761), mouse anti-transferrin receptor (1:500; Invitrogen, #13-6800), rabbit anti-GAPDH antibody (1:2000; Servicebio, GB11002) overnight at 4 °C, then incubated with peroxidase-conjugated goat anti-rabbit IgG (1:10,000 Jackson ImmunoResearch) and goat anti-mouse IgG (1:10,000, Jackson ImmunoResearch) (1h at room temperature). Protein quantitation was completed using ImageJ software (NIH, Bethesda, USA).

Xiong J., Liu Z., Chen S., Kessi M., Chen B., Duan H., Deng X., Yang L., Peng J, & Yin F. (2022). Correlation Analyses of Clinical Manifestations and Variant Effects in KCNB1-Related Neurodevelopmental Disorder. Frontiers in Pediatrics, 9, 755344.

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