Anti cathepsin b antibody

The anti-cathepsin B antibody was from Abcam (Cambridge, UK), the anti-Na⁺/K⁺-ATPase antibody was from MilliporeSigma (Burlington, MA, USA), and the anti-GAPDH and β-tubulin antibodies were from Cell Signaling Technology, Inc (Dover, MA, USA). LipidORDER^TM [31 (link)] was purchased from DiagnoCine (Hackensack, NJ, USA). LysoPrime Green^TM was from Dojindo Molecular Technologies, Inc (Rockville, MD, USA). 4-hydroxy-2-nonenal (HNE) and L-leucyl-L-leucine methyl ester (LLOMe) were purchased from MilliporeSigma. The AAV packaging the C99 YPet-TurquoiseGL (C99 Y-T) [20 (link)] was developed using University of Pennsylvania Gene Therapy Program vector core (Philadelphia, PA, USA; 4.95 × 10¹³ GC/mL).

Anti-Aβ 6E10 and 4G8 antibodies were purchased from BioLegend (San Diego, CA, USA), anti-APP C-terminus antibody was from Sigma-Aldrich (St. Louis, MO, USA), anti-Cathepsin B antibody was from Abcam (Cambridge, UK), anti-Galectin 3 antibody was from Abcam, anti-LAMP1 antibodies were from Sigma-Aldrich and Abcam, anti-nicastrin antibody was from Novus Biologicals (Littleton, CO, USA), and anti-V-ATPase antibody was from Abcam. Normal rabbit IgG antibody was purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), whilst Alexa488 and Cy3-conjugated donkey anti-mouse/rabbit IgG were from Thermo Fisher Scientific (Waltham, MA, USA) and Jackson ImmunoResearch Inc. (West Grove, PA, USA), respectively. DAPT, γ-secretase inhibitor, was purchased from Abcam. DMSO and Chloroquine were purchased from Sigma-Aldrich.