Dulbecco s modified eagle medium dmem

Potassium ferricyanide, ferrous sulfate II, trichloroacetic acid, and sulfuric acid were purchased from Merck (Darmstadt, Germany). Nitro blue tetrazolium (NBT), monosaccharides, diaminoethanetetraacetic acid (EDTA), D-glucose, acid gallic, ascorbic acid, bovine serum albumin protein, ascorbic acid, methionine, 3-(4,5-dimethylthiazolyl-2)-2,5- diphenyltetrazolium bromide (MTT), pyrocatechol violet, riboflavin, ammonium molybdate, and tert-butyl hydrogen peroxide (t-BOOH) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Sodium bicarbonate, nonessential amino acids, and phosphate-buffered saline (PBS) were purchased from Invitrogen Corporation (Burlington, ON, Canada). Dulbecco's Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were obtained from CULTILAB (Campinas, SP, Brazil). Penicillin and streptomycin were obtained from Gibco (Fort Worth, TX, USA). All other solvents and chemicals were of analytical grade from Synth (Diadema, SP, Brazil).

Bullfrog adipose tissue was kindly provided by the Asmarana Produtos Naturais (Natal, RN, Brazil). Span^® 80 (sorbitan monooleate 80), penicillin-streptomycin, and MTT reagent ((3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide)) were purchased from Sigma Aldrich Inc. (St. Louis, MO, USA). Ethanol, dimethylsulfoxide (DMSO), n-hexane, propylene glycol, and Tween^® 20 (polyoxyethylene 20 sorbitan monolaurate) were acquired from VETEC (Rio de Janeiro, RJ, Brazil). Cetostearyl alcohol ethoxylate and isopropyl palmitate were purchased from ViaFarma (São Paulo, SP, Brazil). Xanthan Gum and Germall^® were purchased from Mapric (São Paulo, SP, Brazil). Butylhydroxytoluene was acquired from Galena (Campinas, SP, Brazil). Floral green fragrance was obtained from Bio Inter (São Paulo, SP, Brazil).
Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were purchased from Cultilab (Campinas, SP, Brazil). The 3T3 fibroblasts and B16F10 cell lines from the American Type Culture Collection (ATCC) used in this study were provided by the Laboratory of Biotechnology of Natural Polymers at Federal University of Rio Grande do Norte (Natal, RN, Brazil).

Ethanol, mEthanol, sulfuric acid, potassium ferricyanide, ferrous sulfate (II), and aluminum chloride were obtained from Merck (Germany). Diphenylpicryl hydrazine (DPPH), catechin, and 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Chemical (USA). Sodium chloride was purchased from Sigma Chemical. HeLa cells (ATCC¯ CCL-2), SiHa cells ATCC¯ (HTB-35), 786-0 (ATCC¯ CRL-1932, HT-29 (ATCC¯ HTB-38), and Embryo fibroblast 3T3 (ATCC¯ CCL-164) were provided by Dr. Hugo Oliveira Rocha, Department of Biochemistry, UFRN, Brazil. Cell culture medium components (Dulbecco^'s Modified Eagle Medium, DMEM) and trypsin were obtained from Cultilab (Brazil). Sodium bicarbonate, sodium pyruvate and phosphate buffered saline (PBS) were purchased from Invitrogen Corporation (Canada). All other solvents and chemicals were of analytical grade.