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Chromafil xtra syringe filters

Manufactured by Macherey-Nagel
Sourced in Germany

CHROMAFIL Xtra syringe filters are membrane filters designed for sample preparation. They are available in various membrane materials and pore sizes to accommodate different filtration needs.

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2 protocols using chromafil xtra syringe filters

1

Lignin Nanoparticles with Essential Oils

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Acidolysis lignin (AL) from
beech wood sawdust was isolated as described elsewhere.26 (link) Lignin nanoparticles with entrapped essential
oils (EO-LNPs) were prepared based on the protocols presented in Zikeli
et al.23 (link),26 (link) with the following adaptations: After dissolving
300 mg of AL in 10 mL of DMSO, 150 μL of the respective pure
EOs was added and further mixed for 5 min. After filtering through
0.45 μm CHROMAFIL Xtra syringe filters (Macherey-Nagel GmbH
& Co. KG, Düren, Germany), the solutions were filled in
dialysis bags (SpectraPor 1 Dialysis Membrane Standard RC Tubing,
6–8 kDa, Spectrum Labs, USA) and dialyzed against 4 L of distilled
water for 1 h. A short dialysis time was chosen to prevent a concomitant
release of already entrapped EOs and thus study the very first phase
of EO-LNP formation. The resulting EO-LNP dispersions were stored
in the fridge until further analysis. Aliquots of the EO-LNP dispersions
were freeze-dried for further analysis as well as for in vitro release
experiments.
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2

Optimization of Polymeric Coatings and Substrates

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TMSC (Avicel, Mw = 185,000 g/mol, Mn = 30,400 g/mol, polydispersity index = 6.1 determined by size exclusion chromatography in chloroform) with a DSTMS value of 2.8 was purchased from TITK (Rudolstadt, Germany). chloroform (99.3%) and acetone (99.7%) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Brenntag CEE GmbH (Vienna, Austria). Hydrochloric acid (37%) and sulfuric acid (95%) were obtained from VWR Chemicals (Radnor, PA, USA). 2-Propanol was obtained from Fisher Scientific (Hampton, NH, USA). For the contact angle measurements, Milli-Q water (resistivity = 18.3 Ω−1·cm−1) and diiodomethane were used (Sigma Aldrich, 99%). All chemicals were used without further purification.
The polymer foils Europe 100 economy overhead transparencies were purchased from Avery Zweckform (Oberlaindern, Germany), and PA-6.6 cast film monolayer foils were supplied by BASF SE (Ludwigshafen, Germany). The microstructured copper foil was kindly provided by AT&S (Leoben, Austria), and a nickel foil with a thickness of 40 µm was used. Chromafil Xtra syringe filters made from polyvinylidene difluoride (PVDF)-45/25 0.45 μm pore size (Macherey Nagel, Düren, Germany) were used as obtained.
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