Edu proliferation detection kit

Manufactured by RiboBio

Sourced in China

The EdU proliferation detection kit is a laboratory tool used to detect and quantify cellular proliferation. It relies on the incorporation of the thymidine analog EdU (5-ethynyl-2'-deoxyuridine) into the DNA of dividing cells, which can then be detected using a fluorescent azide. This kit provides a simple and efficient method to label and visualize proliferating cells.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

EdU detection kit (63 citations) EdU kits (10 citations)

6 protocols using edu proliferation detection kit

Cell Proliferation Assays: CCK-8 and EdU

Check if the same lab product or an alternative is used in the 5 most similar protocols

For Cell Counting Kit‐8 (CCK‐8) assay, transfected cells were inoculated at a density of 2 × 10³ cells/well into 96‐well plates and cultivated for 0, 24, 48, 72 and 96 hours. After different incubation times, each well was added with 20 μL of CCK‐8 reagent (Beyotime Institute of Biotechnology) and cultured for another 2 hours. Then, the absorbance at 450 nm was recorded with a standard microplate reader (Scientific MultiskanMK3, Thermo Scientific).
For 5‐ethynyl‐2′‐deoxyuridine (EdU) assay, cell proliferative ability was estimated with the EdU proliferation detection kit (RiboBio) in the light of the manufacturer's protocol. Briefly, cells were treated with EdU for 2 hours and then stained by 4′,6‐diamidino‐2‐phenylindole (DAPI) (Thermo Fisher Scientific). The images of EdU‐positive cells were captured under a fluorescence microscope (Olympus).

Sun Q., Li J., Li F., Li H., Bei S., Zhang X, & Feng L. (2019). LncRNA LOXL1‐AS1 facilitates the tumorigenesis and stemness of gastric carcinoma via regulation of miR‐708‐5p/USF1 pathway. Cell Proliferation, 52(6), e12687.

+ Open protocol

+ Expand

Quantifying Cell Proliferation with EdU

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell proliferation was detected using EdU Proliferation Detection Kit (C10310, Ribobio, Guangzhou, China). After incubated in EdU (5-ethynyl-2’-deoxyuridine) medium for 24 h, the cells were fixed with 4% paraformaldehyde at RT for 10 min. Then, cells were incubated in 2 mg/ml Glycine for 5 min. Following washed with PBS 3 times, the cells were incubated successively with osmotic agent (0.5% PBS of TritonX-100), 1X Apollo® staining reaction solution and osmotic agent (0.5% PBS of TritonX-100). Subsequently, cells were incubated with DAPI for 5 min at RT in dark, followed by 3-time washes in PBS. Three wells were selected for each group of conditions, and three areas per well were selected for fluorescence imaging (n = 9). Afterwards, the number of proliferated cells and the number of cells nuclear stained by DAPI were counted by ImageJ.

Li Z., Peng F., Liu Z., Li S., Li L, & Qian X. (2022). Mechanobiological responses of astrocytes in optic nerve head due to biaxial stretch. BMC Ophthalmology, 22, 368.

+ Open protocol

+ Expand

EdU Proliferation Assay with Fluorescent Staining

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

EdU was conducted with EdU Proliferation Detection Kit (Ribobio, Guangzhou, China) [54 (link)]. Specifically, NHA cells were seeded into 96-well plates with 8 × 10³ in each well. After being incubated with 100 μL 50 μmol/L EdU medium for 2 h, cells were fixed in 4% paraformaldehyde for 30 min, followed by 50 μL 2 mg/mL glycine for 5 min. After cultivating with 100 μL of 0.5% Triton X-100, cells were incubated with 100 μL of 1 × Apollo^® 567 fluorescent staining solution and 100 μL 1 × Hoechst 33,342 reaction solution in a dark environment. Images were collected from THUNDER Imaging Systems (Leica Microsystems, Wetzlar, Germany).

Dang Y., He Q., Yang S., Sun H., Liu Y., Li W., Tang Y., Zheng Y, & Wu T. (2022). FTH1- and SAT1-Induced Astrocytic Ferroptosis Is Involved in Alzheimer’s Disease: Evidence from Single-Cell Transcriptomic Analysis. Pharmaceuticals, 15(10), 1177.

+ Open protocol

+ Expand

Evaluating AUP1 Impact on UM Cell Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

CCK-8 was used to determine how AUP1 affected the ability of UM cells to proliferate. UM cells were grown in triplicate in 96-well microplates with a cell density of 5,000 per well. Following transfection, the cells were treated at 37°C for 2 h with 10 μL of CCK-8 solution (A311-01, vazyme, Nanjing, China) mixed with 90 μL of complete media in each well at 0, 24, 48, 72, or 96 h. Finally, the absorbance of each well was measured at 450 nm using a microplate reader. The EdU test was used as an additional technique to quantify cell proliferation using the EdU proliferation detection kit (Ribobio, Guangzhou, China). In a nutshell, EdU was applied to MuM-2B and OCM-1 cells (2×10⁵ cells per well) for 2 h before they were stained with DAPI (Thermo Fisher Scientific, USA). A fluorescent microscope (Olympus, Japan) was used to take pictures of the EdU-positive cells, which were then processed in ImageJ.

Liu J., Zhang P., Yang F., Jiang K., Sun S., Xia Z., Yao G, & Tang J. (2023). Integrating single-cell analysis and machine learning to create glycosylation-based gene signature for prognostic prediction of uveal melanoma. Frontiers in Endocrinology, 14, 1163046.

+ Open protocol

+ Expand

EdU Proliferation Detection Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

EdU was presented via EdU Proliferation Detection Kit (RIBOBIO, Guangzhou, China). Briefly, after being incubated with 100 μL 50 μM EdU medium for 2 h, the cells were washed with PBS to elute EdU that did not penetrate DNA. The cells were then fixed with 50 μL cell fixation solution at room temperature for 30 min and were incubated with 50 μL 2 mg/mL glycine for 5 min. After washing, the cells were incubated with 100 μL 1×Apollo® staining reaction solution and 100 μL 1×Hoechst 33342 reaction solution at room temperature in the dark for 30 min, respectively. Results were immediately observed.

Ren R., Du Y., Niu X, & Zang R. (2021). ZFPM2-AS1 transcriptionally mediated by STAT1 regulates thyroid cancer cell growth, migration and invasion via miR-515-5p/TUSC3. Journal of Cancer, 12(11), 3393-3406.

+ Open protocol

+ Expand

Investigating Cellular Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

ALO (purity: 99.8%) was purchased from Selleck Chemicals (Houston, TX, USA). N-acetyl-L-cysteine (NAC) was obtained from Sigma-Aldrich (St. Louis, MO, USA). The Super Lumia ECL HRP substrate kit was purchased from Abbkine Inc (Wuhan, China). The EdU proliferation detection kit was purchased from RiboBio Co., Ltd. (Guangzhou, China). The Cell Counting Kit-8 (CCK-8) was obtained from KeyGen Biotech Co., Ltd. (Nanjing, China). The ROS assay Kit was purchased from the Beyotime Institute of Biotechnology (Haimen,China). Primary antibodies against β-actin, GAPDH, p-Src, p-Stat3, p-Akt, Akt, p-mTOR, mTOR, p-P70S6K, P70S6K, p-S6, S6, p-IκB, and gp91^phox were all purchased from Cell Signalling Technology (Beverly, MA, USA). The p47^phox and p65 primary antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX, USA). The p22^phox primary antibody was purchased from ABclonal Biotechnology Co., Ltd (Hubei, China). Secondary antibodies coupled to the IRDye800 fluorophore for use with the Odyssey Infrared Imaging System were purchased from LI-COR Biosciences (Lincoln, NE, USA).Horseradish peroxidase-conjugated anti-mouse IgG and anti-rabbit IgG secondary antibodies were obtained from Cell Signalling Technology (Beverly, MA, USA).

Wang Z., Tang T., Wang S., Cai T., Tao H., Zhang Q., Qi S, & Qi Z. (2020). Aloin Inhibits the Proliferation and Migration of Gastric Cancer Cells by Regulating NOX2–ROS-Mediated Pro-Survival Signal Pathways. Drug Design, Development and Therapy, 14, 145-155.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!