Silica gel, hydrochloric acid fuming 37%, diethyl ether, methanol, toluene, dichloromethane and acetonitrile were acquired from Merck, New Jersey, United States. Meanwhile, 1-methylimidazole, 3-chloropropyltrimethoxysilane (CPTMS), sodium salicylate (97% purity), Ni(ii ) nitrate salt (97% purity) and Pb(ii ) nitrate salt (99.99% purity) were purchased from Sigma-Aldrich, United States. The stock solutions of 200 mg L−1 were prepared individually for Pb(ii ) and Ni(ii ) aqueous solution. Eventually, further dilutions were prepared by diluting the stock solutions with distilled water. Concentration of Pb(ii ) and Ni(ii ) ions in aqueous solution were measured using atomic absorption spectrometer (AAS) from Agilent (Model 240 FS, Agilent Technologies, United States).
Hydrochloric acid fuming 37
Manufactured by Merck Group
Sourced in United States
Hydrochloric acid fuming 37% is a chemical compound used in various industrial and laboratory applications. It is a clear, colorless, and highly corrosive liquid with a pungent odor. The product has a concentration of 37% hydrochloric acid in water.
Automatically generated - may contain errors
Lab products found in correlation
Pb ii nitrate salt, by Merck Group (2 mentions)
1 methylimidazole, by Merck Group (2 mentions)
Silica gel, by Merck Group (2 mentions)
3 chloropropyl trimethoxysilane, by Merck Group (1 mentions)
Model 240 fs, by Agilent Technologies (1 mentions)
Sodium salicylate, by Merck Group (1 mentions)
Sulfuric acid, by Avantor (1 mentions)
4 aminobenzoic acid, by Merck Group (1 mentions)
Sodium nitrite, by Merck Group (1 mentions)
Sodium hydroxide pellet, by Merck Group (1 mentions)
Thiosalicylic acid, by Merck Group (1 mentions)
3 chloropropyltrimethoxysilane cptms, by Merck Group (1 mentions)
Borate buffer, by Agilent Technologies (1 mentions)
O phthalaldehyde reagent, by Agilent Technologies (1 mentions)
9 fluorenylmethylchloroformate reagent, by Agilent Technologies (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
L glutamine, by Agilent Technologies (1 mentions)
Sodium hydroxide pellets, by Thermo Fisher Scientific (1 mentions)
Sybr safe dna gel stain, by Thermo Fisher Scientific (1 mentions)
Human stat3 sirna, by Horizon Discovery (1 mentions)
7 protocols using hydrochloric acid fuming 37
1
Adsorption of Heavy Metal Ions
2
Electrochemical Analysis of Mild Steel
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Two mild steel (MS) samples containing 0.17% C, 0.045% S, 1.40% Mn, 0.009% N and remainder Fe were used in this study. The first sample was a cylindrical disc with 0.5 cm2 surface area, which was used as the working electrode for electrochemical experiments. The second sample was a 1 × 1 × 0.1 cm3 plate of MS, which was used for SEM-EDX and contact angle analysis.
Sulfuric acid 95% was purchased from VWR. Hydrochloric acid fuming 37%, 4-aminobenzoic acid and sodium nitrite were purchased from Merck. Double distilled water was used to prepare all solutions.
Sulfuric acid 95% was purchased from VWR. Hydrochloric acid fuming 37%, 4-aminobenzoic acid and sodium nitrite were purchased from Merck. Double distilled water was used to prepare all solutions.
3
Synthesis of Pb(II)-Imidazole Complex
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Silica gel, sodium hydroxide pellets (98% purity), hydrochloric acid fuming 37%, diethyl ether, methanol, toluene, dichloromethane, and acetonitrile were acquired from Merck, NJ, USA. Meanwhile, 1-methylimidazole, 3-chloropropyltrimethoxysilane (CPTMS), thiosalicylic acid (97% purity), and Pb(II) nitrate salt (99.99% purity) were purchased from Sigma-Aldrich, St. Louis, MO, USA.
4
Amino Acid Analysis by HPLC
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Hydrochloric acid fuming %37 (HCl), sodium phosphate dibasic solution (Na2HPO4), sodium hydroxide (NaOH), methanol (MeOH; GC grade) and acetonitrile (ACN; GC grade) were purchased from Merck. Borate buffer, o-phthalaldehyde reagent (OPA), 9-fluorenylmethyl chloroformate reagent (FMOC), amino acid standard solutions (mix of L-alanine, Larginine, L-aspartic acid, L-cystine, L-glutamic acid, glycine, Lhistidine hydrochloride monohydrate, L-isoleucine, L-leucine, L-lysine hydrochloride, L-methionine, L-phenylalanine, Lproline, L-serine, L-threonine, L-tyrosine, L-valine stored in 0.1N HCl with different dosages as 1nmol, 250pmol, 100pmol, 25pmol and 10pmol) and extended amino acid standards for sensitive ones such as L-glutamine, L-asparagine, Ltryptophan and L-4-hydroxyprolin in the form of powder were obtained from Agilent Technologies. Water purified (18.1 megaohm purity) with Thermo Scientific Nanopure. Amino acid column for HPLC system (Zorbax clip AAA, 5µm, 4.6x150m), 1.5ml amber vials with politetrafloroetilen caps (PTFE) and syringe filters (0.45µm) were also purchased from Agilent Technologies.
5
STAT3 Silencing Using siRNA
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Human STAT3-siRNA (1385 atoms and 13,330.21 Da) of the following sequence was provided by Dharmacon Inc. (Horizon Discovery Ltd., Waterbeach, UK): (sense strand: 5’-GGAGCAGCACCUUCAGGAUdTdT-3’; antisense strand: 5’-AUCCUGAAGGUGCUGCUCCdTdT-3’). GeneRuler Ultra Low Range DNA Ladder containing TriTrac DNA Loading Dye (6X) was purchased from Thermo Fisher Scientific (Vilnius, Lithuania). Agarose, Quant-iTTM RiboGreen® RNA Reagent and Kit, SYBR Safe DNA gel stain, UltraPureTM TBE Buffer, 10X and UltraPureTM DNase/RNase-Free Distilled Water were provided by Invitrogen (Thermo Fischer Sci., Carlsbad, CA, USA). Methanol (HPLC grade MeOH), ≥99.8% was provided by Fischer Scientific (Thermo Fischer Sci., Loughborough, UK). Sodium chloride (NaCl) for the analysis was obtained from Applichem GmbH (Darmstadt, Germany). 4-Methylphtalalic anhydride 96%, phosphoric acid, extra pure, 85 wt% solution in water, sodium hydroxide pellets, 98.5%, were purchased from Acros Organics (Geel, Belgium). Hydrochloric acid fuming ≥ 37% (HCl), HEPES (N-[2-Hydroxyethyl)piperazine-N’-[2-ethanesulfonic acid]) sodium salt, poly(ethylene glycol) average Mn 6000 (PEG), Trizma® hydrochloride (TRIS HCl) and Tween 20 were obtained from Sigma Aldrich (St-Louis, MO, USA).
6
Stem Cell Differentiation Culture Protocol
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DMEM supplemented with HAM’S-F12 (DMEM/F12, ref: D8437), penicillin/streptomycin (P/S, ref: P4458), L-glutamine (ref: G7513), fetal bovine serum (FBS, ref: F7524), human apo-transferrin (ref: T4382-100MG), sodium selenite (ref: 214485-100G), TRI Reagent and RNA Isolation Reagent (ref: T9424-200ML), 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, ref: 475989), hydrochloric acid fuming 37% (ref: 1003171011), MPH CII (ref: 1433008), and the primers listed in Table 1 were purchased from Sigma-Aldrich (St. Louis, MO, USA).
E.Z.N.A.® Total RNA Kit I (ref. R6834-01) was purchased from Omega Bio-Tek (Atlanta, USA). RNase-Free DNase I (ref. D9910K) was purchased from Cientisol (A Coruña, Spain). High-Capacity cDNA Reverse Transcription Kit was purchased from Applied Biosystems Life Technologies (Waltham, MA, USA). iTaq Universal SYBR Green Supermix (ref: 1525125) was purchased from BioRad (Hercules, CA, USA). We purchased 96-well (ref: 3798) and 100 mm (ref: 430167) adherent culture plates from Corning (Phoenix, AZ, USA). Insulin (ref: 775502) was purchased from Novo Nordisk Pharma (Madrid, Spain).
E.Z.N.A.® Total RNA Kit I (ref. R6834-01) was purchased from Omega Bio-Tek (Atlanta, USA). RNase-Free DNase I (ref. D9910K) was purchased from Cientisol (A Coruña, Spain). High-Capacity cDNA Reverse Transcription Kit was purchased from Applied Biosystems Life Technologies (Waltham, MA, USA). iTaq Universal SYBR Green Supermix (ref: 1525125) was purchased from BioRad (Hercules, CA, USA). We purchased 96-well (ref: 3798) and 100 mm (ref: 430167) adherent culture plates from Corning (Phoenix, AZ, USA). Insulin (ref: 775502) was purchased from Novo Nordisk Pharma (Madrid, Spain).
7
Bevacizumab Characterization by FabRICATOR
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Vials of bevacizumab (Avastin ® , Genentech, Inc., San Francisco, CA, USA) 25 mg/mL were kindly provided by the Hospital Pharmacy of the University Hospitals of Geneva (Geneva, Switzerland). FabRICATOR ® (IdeS protease) was obtained from Genovis AB (Lund, Sweden). β-Mercaptoethanol (β-MEA), DL-dithiothreitol (DTT), ethylenediaminetetraacetic acid disodium salt (EDTA), Hydrochloric acid fuming ≥37% (HCl), tris-hydroxymethylaminomethane (TRIS), formic acid (FA ≥ 99%), and trifluoroacetic acid (TFA ≥ 99%) were purchased from Sigma-Aldrich (St Louis, MO, USA). UHPLC-MS grade acetonitrile (ACN) and water were supplied by Biosolve (Valkenswaald, The Netherlands).
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