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Dihydroouabain

Manufactured by Merck Group
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Sourced in United States, Germany

Dihydroouabain is a laboratory reagent used in scientific research. It is a cardiac glycoside, a class of compounds that affect the heart and circulatory system. The core function of dihydroouabain is to serve as a tool for studying ion transport and cellular mechanisms in biological systems.

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Lab products found in correlation

Nifedipine, by Merck Group (2 mentions) Niflunic acid, by Merck Group (2 mentions) Nicl2, by Merck Group (2 mentions) Apamin, by Alomone (2 mentions) Lidocaine, by Merck Group (2 mentions) Chromanol 293b, by Merck Group (2 mentions) E 4031, by Merck Group (2 mentions) Digitoxin, by Merck Group (1 mentions) Aldosterone, by Merck Group (1 mentions) Digoxin, by Merck Group (1 mentions) Oleandrin, by Merck Group (1 mentions) Digitoxigenin, by Merck Group (1 mentions) Ouabain, by Merck Group (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) N6 2 o dibutyryladenosine 3 5 cyclic monophosphate sodium salt dbcamp, by Merck Group (1 mentions) Na k atpase α1, by Abcam (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Glibenclamide, by Merck Group (1 mentions)

3 protocols using dihydroouabain

1

Comprehensive Protein Assays and Compound Characterization

Cited 1 time
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These assays were performed as described (Yang et al., 2007 (link)) using antibodies against GAPDH, β-actin, and caspase-3 (Cell Signaling Technology Inc., MA, USA); and Na+/K+-ATPase α1 (Abcam Inc. Cambridge, UK). Tylophorine and Reevesiosides A-I were prepared as described (Chang et al., 2013 (link), Yang et al., 2010 (link)). DMSO (D1435, ≧ 99.5%), digoxin (D6003, ≧ 95%, HPLC), digitoxin (D5878, ≧ 92%, HPLC), digitoxigenin (D9404, 99%, TLC), ouabain (O3125, ≧ 95%, HPLC), dihydroouabain (D0670, ≧ 98.5%, TLC), oleandrin (O9640, ≧ 98%, HPLC), N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (Db cAMP) (D0627, ≧ 96%, HPLC), and aldosterone (A9477, ≧ 95%, HPLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Yang C.W., Chang H.Y., Hsu H.Y., Lee Y.Z., Chang H.S., Chen I.S, & Lee S.J. (2017). Identification of anti-viral activity of the cardenolides, Na+/K+-ATPase inhibitors, against porcine transmissible gastroenteritis virus. Toxicology and Applied Pharmacology, 332, 129-137.
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2

Pharmacological Modulation of hiPSC-CMs

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Vernakalant was from Sigma-Aldrich (Taufkirchen, Germany). The drug was applied to a cell by a perfusion pipette, from low to high concentrations. The used concentrations were determined according to previous or our preliminary studies in hiPSC-CMs. E-4031, chromanol 293B, nifedipine, NiCl2, glibenclamide, niflunic acid, lidocaine and dihydroouabain were from Sigma-Aldrich, 4-AP from RBI, apamin from Alomone Labs, tetrodotoxin (TTX) from Carl Roth (Karlsruhe, Germany). E-4031, NiCl2, TTX, 4-AP, apamin, niflumic acid and dihydrooubain were dissolved in H2O. nifedipine, and chromanol 293B were dissolved in DMSO, lidocaine in ethanol. Stock solutions were kept at −20 °C.
Xu Q., Huang X., Meng Z., Li Y., Zhong R., Li X., Cyganek L., El-Battrawy I., Akin I., Zhou X, & Lan H. (2022). Antiarrhythmic Effects of Vernakalant in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes from a Patient with Short QT Syndrome Type 1. Journal of Cardiovascular Development and Disease, 9(4), 112.
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3

Characterization of Cardiac Ion Channels

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Disopyramide is from SigmaAldrich. The drug was applied to a cell sequentially from low to high concentrations by a perfusion pipette. The tested concentrations were selected according to previous or our preliminary studies in hiPSC-CMs. E-4031, chromanol 293B, nifedipine, NiCl2, niflunic acid, lidocaine, and dihydroouabain are from Sigma Aldrich, 4-AP from RBI, apamin from Alomone Labs, TTX from Carl Roth. E-4031, NiCl2, TTX, 4-AP, apamin, niflumic acid, and dihydrooubain were dissolved in H2O. nifedipine, and chromanol 293B were dissolved in DMSO, lidocaine in ethanol. Stock solutions were kept at -20 °C.
Lan H., Xu Q., El-Battrawy I., Zhong R., Li X., Lang S., Cyganek L., Borggrefe M., Zhou X, & Akin I. (2020). Ionic Mechanisms of Disopyramide Prolonging Action Potential Duration in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes From a Patient With Short QT Syndrome Type 1. Frontiers in Pharmacology, 11, 554422.
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